báo cáo hóa học:" A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" pdf

... not for citation purposes) Virology Journal Open Access Methodology A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame Ermei Yao 1 , ... efficient amplification. The HCV ORF was therefore partitioned into amplicons 1, 2, 3, 4x and 4y for genotype 1a and amplicons 1x, 1y, 2, 3, 4x...

Ngày tải lên: 20/06/2014, 04:20

... efficiency for patients' amplicons Genotype 1a Amplicon A1 A2 A3 A4 x A4 y Amplification efficiency 95 a 98 93 10 0 95 Average efficiency 96.2 Genotype 1b Amplicon A1 x A1 y A2 A3 A4 x A4 y Amplification ... (see additional file 2: HCVMethodPaperTable2.xls) list amplification and sequencing primers for genotypes 1a and 1b. Table 3 (see additional file...

Ngày tải lên: 19/06/2014, 08:20

... from 11 to 20. Therefore, for block 1 11 n = 14 and for block 12 –20 n = 13 , 11 , 11 , 10 , 9, 8, 6, 3 and 1 respectively. For each box plot, the whiskers represent maximum and minimum values, the ... the top and bottom of the box represent the 75th and 25th quartiles and the horizontal line in the box represent the median. 0 5 10 15 20 25 30...

Ngày tải lên: 19/06/2014, 08:20

... system offers the potential of naturalistic pacing, it still features somewhat unnatural control methods (hand, foot, and tongue motor imagery), as well as variable accuracy and high computational demand. ... identified the more effective channels and frequency bands for control. We calculated each Bhattacharyya dis- tance according to (1) , where M i and Σ i are the mean vec...

Ngày tải lên: 19/06/2014, 08:20

... data analysis and the preparation of the manuscript. MJS supervised the study plan- ning, data analysis and preparation of the manuscript. All authors read and approved the final manuscript. Acknowledgements The ... microscopic analysis and helped with the planning and prepara- tion of the manuscript. PIF and AES participated in the cutting and staining of the...

Ngày tải lên: 20/06/2014, 04:20

... different forms of eval- uation research within the U.K. National Health Service. Qualitative Health Research 2002, 12 :966-9 81. 55. Catterall M, Maclaran P: Focus group data and qualitative anal- ysis ... quantitative and qualitative meth- ods in language research and intervention. American Journal of Speech-Language Pathology 2003, 12 :16 5 -17 1. 65. Arborelius E, Timpka T: Ge...

Ngày tải lên: 20/06/2014, 15:20

... with inorganic nanomaterials: Preparations and applications. Coord Chem Rev 2 010 , 254 :11 17, and the reference there in. 3. Banerjee S, Wong SS: Synthesis and Characterization of Carbon Nanotube-Nanocrystal ... 11 3 :18 4 31. doi :10 .11 86 /15 56-276X-6- 71 Cite this article as: Zhao et al.: A general strategy for synthesis of metal oxide nanoparticles attached on carbon...

Ngày tải lên: 21/06/2014, 06:20

... that for all x, y Î C and l >0, Jung Journal of Inequalities and Applications 2 011 , 2 011 : 51 http://www.journalofinequalitiesandapplications.com/content/2 011 /1/ 51 Page 4 of 23 and so (1 − α n )||x n − ... Journal of Inequalities and Applications 2 011 , 2 011 : 51 http://www.journalofinequalitiesandapplications.com/content/2 011 /1/ 51 Page 14 of 23 where {a n }, {...

Ngày tải lên: 21/06/2014, 00:20

... lithography-free method of micro/nanoscale fabrication and patterning on Si and Ge surfaces Nanoscale Research Letters 2 012 , 7 :11 0 doi :10 .11 86 /15 56-276X-7 -11 0 Huatao Wang (wanghuatao1@gmail.com) Tom ... square, and wire were observed on Ge (11 1), Ge (10 0), and Ge (11 0) substrates, respectively, and all the nanopits have the same orientation in a given sample....

Ngày tải lên: 21/06/2014, 17:20

... 5¢-GTTCACCACCAGAAGCT GGTGTTCTTC GCTGAA GACGT GGGTTCT AACAAG GGTGCT-3¢;Abc, 5¢-CACAACGCCACCAACCATCAGA CCGATGATAGCACCCTTGTTAGAACCCAC-3¢;Ab- start, 5¢-GCGTAGGGTCGACATATGGACGCTGAATT CCGTCACG-3¢;Abstop, ... SEC- isolated Ab (1 40) and Ab(M1–40) are at least 97% pure. In the lanes containing Ab (1 42) and Ab(M1– 42), there were prominent bands at approximately 4 kDa and faint bands at appro...

Ngày tải lên: 18/02/2014, 13:20