a general method for synthesising iron ii complexes

... Birmingham and Basel has given great support for my research work Thanks for the help and friendship from all my colleagues: Tao, Pan, Valerie J, Ayten, Robyn, Azad, Chris, Annette, Amar, Barbara, ... 308 Appendix VI: Tables of H NMR spectroscopic data and ES-MS spectra of the ruthenium (II) and iron( II) metallomacrocycles Curriculum Vitae 311 323 v Abbreviations Abbreviations General Ant A sol ... Huc, and J.-M Lehn, Proc Natl Acad Sci USA, 1997, 94, 2106 143 V Goral, M I Nelen, A V Eliseev, and J.-M Lehn, Proc Natl Acad Sci USA, 2001, 98, 1347 144 S Sakai, Y Shigemasa, and T Sasaki, Tetrahedron...

Ngày tải lên: 02/07/2014, 13:33

... a- amino-b-carboxymuconate-e-semialdehyde decarboxylase J Am Chem Soc 129, 9278–9279 Fukuoka SI, Ishiguro K, Yanagihara K, Tanabe A, Egashira Y, Sanada H & Shibata K (2002) Identification and expression of a cDNA encoding human 6622 ... a- amino-b-carboxymuconate-e-semialdehyde decarboxylase (ACMSD) J Biol Chem 277, 35162–35167 Tanabe A, Egashira Y, Fukuoka SI, Shibata K & Sanada H (2002) Expression of rat hepatic 2-amino3-carboxymuconate-6-semialdehyde ... potential interest to reduce life-threatening complications of cerebral malaria, and as an important tool in validating our proposal of hACMSD as a novel drug target for the treatment of diabetes and...

Ngày tải lên: 18/02/2014, 06:20

... were amplified by PCR using pairs of primers 5¢-CATATGCAACAGACAACCCAAATTCAGCCC-3¢ and 5¢-AGATCTTATCACTCCCTTACTAAGTCGATG-3¢ for the b subunit and 5¢-CATATGAGCGAGAAAACCA TGCGCGTGCAG-3¢ and 5¢-AGATCTTAGCTTCCTTT ... DNA polymerase (Stratagene) and pairs of primers 5¢-TCTGAGTGCGGTGGAAGAGATG ATGAAGCG-3¢ and 5¢-AGATCTTATTCAATGGTGT CGGGCTGAACC-3¢ and digested with EcoRV and BglII Resulting 210-bp fragment was ... Crystalline AdoCbl was a gift from Eizai, Tokyo, Japan DEAE-cellulose was purchased from Wako, Osaka, Japan The other chemicals were analytical grade reagents Preparation of expression plasmids for...

Ngày tải lên: 21/02/2014, 03:20

... dimer at both weakly acidic pH (6.0) and weakly basic pH (8–9) Table DASA in dimer formation The DASA of the AB dimer is defined as [(ASA of A) + (ASA of B) ) (ASA of AB dimer)] ⁄ The number of water ... dimer interface was detected with ASV CALCULATOR [34] ASA was calculated with a program kindly provided by M Maeda (National Institute of Agrobiological Sciences, Japan) ˚ DASA (A2 ) AB dimer BC ... difference in accessible surface area (DASA) in each dimer was calculated (Table 1) It is conceivable that a dimer with larger DASA is more plausible We found that the DASAs of the AB and DA dimers...

Ngày tải lên: 14/03/2014, 23:20

... Collaborative Computational Project N (1994) The CCP4 suite: programs for protein crystallography Acta Crystallogr D 50, 760–763 18 Navaza J (1994) AMoRe: an automated package for molecular replacement ... Diffraction data were collected at 100 K on Beamline BL44B2 at SPring-8 (Harima Institute, Hyogo, Japan) using an ADSC Quantum 210 CCD detector (Area Detector Systems ˚ Corporation, Poway, CA, USA) ... procedure (Stratagene) with primers 5¢-TCTTCAGCGGC ATGGGCGACCTCGGCGGCAT-3¢ and 5¢-ATGCCGC CGAGGTCGCCCATGCCGCTGAAGA-3¢ The recombinant protein was purified using a HiTrapÔ chelating column and Resource...

Ngày tải lên: 23/03/2014, 05:22

... Glu272–Arg148 21 19 14 092 8248 5844 –1 6.5 No of hydrogen bonds (side chain–side chain) Aromatic stacking ˚ ASA total (A2 ) ˚ ASA apolar (A2 ) ˚ ASA polar (A2 ) Formal global charge Theoretical pIb ... class C b-lactamase C Michaux et al classes A, C and D are active site serine enzymes, whereas class B b-lactamases require one or two zinc ions for their activity [1] Only class C b-lactamases ... in catalysis, with a concomitant reduction in stability of coldadapted enzymes [15,16] A general theory for cold adaptation has not been formulated yet, as different enzymatic families can follow...

Ngày tải lên: 23/03/2014, 07:20

... oscillation one degree for the respective crystals The crystal parameters and data collection statistics are given in Table below Table Crystal parameters and data-collection statistics for wt AtCyP38 ... obtained by vapor batch method in a condition having PEG6000 and t-butanol as precipitants Crystals of selenomethionylated AtCyP38 protein was obtained in the same condition as that of native AtCyP38 ... in the crystal and no part of it has got cleaved off during the crystallization process 4.5.2 Data collection and analysis The native and selenomethionylated crystal data sets for all the five...

Ngày tải lên: 14/09/2015, 17:50

... report, partially purified AmyA that contained the signal peptide with a His-tag was used for the activity assay From the crystal structure of AmyA it appears that the His-tag and Nterminal signal ... To address these questions we have undertaken a biophysical study of AmyA, a secretory α-amylase (Mijts and Patel, 2002) from Halothermothrix orenii AmyA is an endo-acting α-amylase and randomly ... salt at molar levels and temperature above 60 °C makes AmyA a true halo-thermophilic enzyme 2.1.4 The α-amylases: Alpha-Amylases (alpha-1,4-glucan-4-glucanohydrolase, EC 3.2.1.1) are Endo-acting...

Ngày tải lên: 16/09/2015, 08:31

... in the most favourable region of the Ramachandran plot (Table 2) Crystal structure of a Thielavia arenaria laccase Kinetic data Kinetic constants (Km and Vmax) for rMaL, TaLcc1 and ThL were determined ... bleaching, even at high temperatures and at neutral pH [32] In general, asco-laccases possess a wider optimal pH range than basidiomycete laccases; however, the catalytic ability of asco-laccases ... Journal compilation ª 2011 FEBS 2293 Crystal structure of a Thielavia arenaria laccase J P Kallio et al 15 Lyashenko AV, Zhukova Y, Zhukhlistova N, Zaitsev V, Stepanova E, Kachalova G, Koroleva...

Ngày tải lên: 14/02/2014, 18:20

... those for ˚ ˚ importin -a: 36.9 A2 for main-chain atoms, 40.2 A2 for ˚ overall (62 total atoms) side-chain atoms and 38.7 A Electron density analysis Both the main-chain and side-chain atoms of ... that it adopts the canonical glutathione S-transferase fold with an N-terminal thioredoxin-like domain and an a- helical C-terminal domain [4] CLIC4 can form poorly selective anion channels that ... have a zero mean and unit variance All atoms that satisfy Bz ‡ are treated as outliers and discarded After discounting outliers, Bz values were recalculated The normalized B factors from apo importin-a...

Ngày tải lên: 14/02/2014, 19:20

... hyperthermophilic archaeon growing at temperatures up to 100°C Int J Syst Bacteriol 46, 1070–1077 10 Kawarabayasi Y, Hino Y, Horikawa H, Yamazaki S, Haikawa Y, Jin-no K, Takahashi M, Sekine M, Baba S, Ankai A ... Radiation Research Center (Taiwan, ROC) We thank Ms M.Sakai (Osaka University) for performing ultracentrifugation analysis and Mr K Mieda and M Sakata (Tottori University) for technical assistance in ... dismutase tyrosine 34 to structure and catalysis Biochemistry 48, 3417–3424 Nakamura T, Matsumura H, Inoue T, Kai Y, Uegaki K, Hagihara Y, Ataka M & Ishikawa K (2005) Crystallization and preliminary...

Ngày tải lên: 14/02/2014, 22:20

... substrate-free PhyK with substrate-free ˚ AppA the Ca atoms are 2.41 A apart, whereas for the substrate-free PhyK and the substrate-loaded AppA ˚ the averaged distance is only 1.87 A Distinct conformational ... fewer charges whereas AppA undergoes a distinct conformational change upon substrate binding Classification of HAPs Induced conformational changes upon substrate binding For a detailed structural superposition, ... AppA by a factor of  2200 The conformational changes of AppA upon substrate binding facilitate a faster turnover of phytate and are in line with a higher specificity The relatively stiff catalytic...

Ngày tải lên: 16/02/2014, 09:20

... growth factors and proteinase inhibitors Biol Chem 380, 473–483 Kataoka H, Uchino H, Asada Y, Hatakeyama K, Nabeshima K, Sumiyoshi A & Koono M (1997) Analy- Crystal structure of the catalytic domain ... proteinase domain of DESC1, rapid amplification of cDNA ends reactions were performed on a human prostate Marathon-Ready cDNA (Clontech, Mountain View, CA, USA) Two fragments were isolated and confirmed ... most favored and favored regions of the Ramachandran ˚ plot and r.m.s.d values for bond and angle of 0.005 A and 1.37 ° as shown in Table References Table Data collection and refinement statistics...

Ngày tải lên: 19/02/2014, 00:20

... of GAB catalysis have been identified to date [12] which are based either on a single, bifunctional GAB catalyst or on a GAB catalysts pair The available biochemical data on MshB and LpxC are ... Sorokin A, Anderson I, Galleron N, Candelon B, Kapatral V, Bhattacharyya A, Reznik G, Mikhailova N, Lapidus A et al (2003) Genome sequence of Bacillus cereus and comparative analysis with Bacillus anthracis ... Solvent-accessible surface [15] ˚ calculations show that a substantial area, 1677 A2 per chain, is buried upon dimer formation In the crystal, BcZBP is a hexamer formed by three dimers that are related...

Ngày tải lên: 19/02/2014, 00:20

... atoms of Asp11 and Asn23 (Fig 3) in an arrangement similar to what is observed in VPRK Both PRK and VPRK have calcium bound at Ca3 SPRK also has an aspartic acid residue at position at 200, and ... hexa62 Table Data collection and refinement statistics for SPRK ˚ Resolution (A) Space group Cell parameters ˚ a- axis (A) ˚ b-axis (A) ˚ c-axis (A) b angle (°) Number of observations (24 – maximum ... initial comparative studies showed that the catalytic turnover was at least twice that of PRK, but substrate affinity was reduced SPRK was compared with PRK and was found to be remarkable stable against...

Ngày tải lên: 19/02/2014, 07:20

... hydrogen bonds Main chain–main chain Main chain–side chain Side chain–side chain Total ˚ Exposed surface areab (A2 ) ˚ Apolarc (A2 ) ˚ Buried surface areab (A2 ) ˚ Apolarc (A2 ) a 1IC6 1THM 38 24 ⁄ ... uracil–DNA glycosylase from Atlantic cod (Gadus morhua) reveals cold-adaptation features Acta Crystallogr Sect D 59, 1357–1365 10 Aghajari N, Van Petegem F, Villeret V, Chessa JP, Gerday C, Haser ... thank Andrea Schmidt for assistance during data collection at DESY beamline BW 7A and Achim Dickmanns for comments on the manuscript References Blochl E, Rachel R, Burggraf S, Hafenbradl D, Jannasch...

Ngày tải lên: 19/02/2014, 16:20

... 5¢-CAACAAATTTGCATATGACTATT AAAG-3¢ containing an NdeI site (underlined) next to the start codon of the T brucei gGAPDH gene; an antisense primer 5¢-CAGCCAAGCGCCTAGGGAGCGAGA AC-3¢, containing a BamHI ... oscillation mode Methods Enzymol 276, 307–326 Navaza, J (1994) AMoRe: an automated package for molecular replacement Acta Crystallogr A 50, 157–163 Brunger, A. T., Adams, P.D., Clore, G.M., Delano, ... Castellano, E.E., Garratt, R.C & Craivich, A (1998) Set-up and experimental parameters of the protein crystallography beamline at the Brazilian National Synchrotron Laboratory J Synchr Rad 5,...

Ngày tải lên: 20/02/2014, 02:21

... Marion-Poll, A. , Caboche, M., Kamiya, Y & Koshiba, T (1998) Molecular cloning and characterization of aldehyde oxidases in Arabidopsis thaliana Plant Cell Physiol 39, 433–442 Koga, J., Adachi, T & Hidaka, ... Enterobacter cloacae indolepyruvate decarboxylase, and K.-P Rucknagel (Max¨ Planck-Society, Research Unit ÔEnzymology of protein foldingÕ, Halle/ Saale, Germany) for the amino acid sequence analysis ... Suite: Programs for Protein Crystallography Acta Crystallogr D50, 760–763 Lu, G (1999) PATTERN: a precession simulation programme for displaying reciprocal-space diffraction data J Appl Crystallogr...

Ngày tải lên: 20/02/2014, 11:20

... salt-tolerant glutaminase K Yoshimune et al asparaginase in complex with l-glutamate (PDB 2hln) [18] Mglu binds l-glutamate in an extended form ˚ (Fig 7A) with a contact area of 288 A2 Asparaginase ... 765–773 Wakayama M, Yamagata T, Kamekura A, Boontim N, Yano S, Tachiki T, Yoshimune K & Moriguchi M (2005) Characterization of salt-tolerant glutaminase from Stenotrophomonas maltophila NYW-81 and ... salt-tolerant glutaminase K Yoshimune et al 32 Chantawannakul P, Yoshimune K, Shirakihara Y, Shiratori A, Wakayama M & Moriguchi M (2003) Crystallization and preliminary X-ray crystallographic studies...

Ngày tải lên: 06/03/2014, 09:22

... aaagtgaagtcc-3¢ and 5¢-attattggatccctattgctggtccgattctgccag-3¢ 3-TPR NlpI primers (residues 62–197) were 5¢-aataatccatgg gggcacagcttttatatgagcgcggag-3¢ and 5¢-aataatggatcctcactgttc cttatccgatttttcgaagtgc-3¢ ... chemically synthesized by the W M Keck Core Facility (Yale University, New Haven, CT, USA) Primers to amplify mature NlpI (residues 20–294) were 5¢-aataatccatggggagtaatacttcctggcgta aaagtgaagtcc-3¢ ... Helsingin Sanomat Centennial Foundation (Finland) Data for this study were measured at beamline X12C of the National Synchrotron Light Source We are grateful for the assistance of Dr Anand Saxena in...

Ngày tải lên: 07/03/2014, 16:20