... machefinehaifatto@libero.it; Alfonso Campanile - facampanile@libero.it; Gennaro Galasso - gengalas@unina.it; Barbara Ziaco - barbara.ziaco@unina.it; Giovanna Giuseppina Altobelli - ggaltobe@unina.it; Vincenzo Cimini ... were evaluated by analy- sis of variance (ANOVA). Statistical Analysis All data are presented as the mean value ± SEM. Statistical differences were determined by one-wa...
Ngày tải lên: 18/06/2014, 15:20
... in the quality of RNA, sample loading and the amount of input cDNA. Amplifi- cation of HGPRT was performed using the primers 5'- TGA CAC TGG CAA AAC AAT GCA-3' (sense) and 5'- GGT ... may occur also in other epithelial cancers [18], we performed a comparative staining of HER-2 and htid in non mammary carcinomas characterized by elevated levels of ErbB-2...
Ngày tải lên: 18/06/2014, 16:20
Báo cáo khoa học: In vivo production of catalase containing haem analogues pot
... present in Fe-PP-KatA and Ga-PP-KatA was extracted from the isolated catalase proteins by using acid ⁄ acetone, and analysed by RP-HPLC. The porphyrin of Ga-PP-KatA eluted as a single peak at 7.0 min. ... except for Ni-PP, indicating that various haem analogues can be inserted into the protein. The catalase proteins were isolated by the same procedure as used for Fe-PP-Kat...
Ngày tải lên: 06/03/2014, 11:20
Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis pot
... and 5¢-GTGAGACACCAGAGTGCAAGA-3¢ (reverse); pri- mers for p53:5¢-CACAGTCGGATATGAGCATC-3¢ (forward) and 5¢-GTCGTCCAGATACTCAGCAT-3¢ (reverse) and primers for cyclin D1:5¢-TGTTCGTGGC CTCTAAGATGA-3¢ (forward) ... which the variances were unequal, the data were adequately transformed before the ANOVA . The null hypothesis was accepted for all the values of these sets in which t...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx
... the particular fatty acid may be different for each species, activation of the plasma membrane H + -ATPase by increases in the unsaturation of the CLB-fatty acids is a physiological and relevant ... rno Vasco, Spain. REFERENCES 1. Nishi, T. & Yagi, T. (1992) A transient and rapid activation of plasma-membrane ATPase during the initial stages of osmoregu- lation...
Ngày tải lên: 08/03/2014, 22:20
Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
... in brain, the natural inhibitor of calpain, calpastatin, was preferentially converted into still active 15 kDa fragments (Table 1), whereas, in aorta, the inhibitor was predominantly inactivated. ... inactivated. As both the inactivation and fragmentation of calpastatin are known to be produced by active calpain [32], these observations further indicate that calpain is activated...
Ngày tải lên: 30/03/2014, 04:20
Báo cáo khoa học: In vivo cross-linking of nucleosomal histones catalyzed by nuclear transglutaminase in starfish sperm and its induction by egg jelly triggering the acrosome reaction pdf
... [6]. Well-documented examples of transglutaminases include plasma factor XIIIa [7], keratinocyte transglutaminase [8], epidermal transglutaminase [9], tissue transglutaminase [10], and prostatic transglutaminase ... F., Yamada, M., Miyata, T., Ding, Y L., Hiranaga- Kawabata, M., Muta, T., Iwanaga, S., Ichinose, A. & Davie, E.W. (1993) Limulus hemocyte transglutaminase. Its purification...
Ngày tải lên: 31/03/2014, 07:20
báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... 117:587-595. 25. Koido S, Hara E, Homma S, Torii A, Mitsunaga M, Yanagisawa M, Toyama Y, Kawahara H, Watanabe M, Yoshida S, Kobayashi S, Yanaga K, Fujise K, Tajiri H: Streptococcal preparation OK-432 pro- motes ... Ministry of Education, Cultures, Sports, Science and Technology of Japan, Grant -in- Aid of the Japan Medical Association, Takeda Science Foundation, Pancreas Research F...
Ngày tải lên: 18/06/2014, 15:20
báo cáo hóa học:" Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson''''s disease" doc
... volume. cDNA was used as template in the following PCR assay. The primers used for PCR assays were as follows: (1) DDC, forward: 5'-TTACTCATCCGATCAGGCACAC-3', reverse: 5'-GGCAGAACAGTCAAAATTCACC-3'; ... Shukla S, Agrawal AK, Chaturvedi RK, Seth K, Srivastava N, Sinha C, Shukla Y, Khanna VK, Seth PK: Co-transplantation of carotid body and ventral mesencephalic cell...
Ngày tải lên: 18/06/2014, 15:20
báo cáo hóa học:" Mass spectrometry-based serum proteome pattern analysis in molecular diagnostics of early stage breast cancer" pdf
... corre- sponding signal abundances, covering the range of ana- lyzed m/z values. Analysis of protein tumor markers in plasma Plasma samples were obtained after centrifugation of blood on a Ficoll gradient ... Statistical Analysis The preprocessing of data that included averaging of tech- nical repeats, interpolation of missing or non-aligned points, binning of neighbo...
Ngày tải lên: 18/06/2014, 15:20