Báo cáo sinh học: " A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" potx

... efficiency for patients' amplicons Genotype 1a Amplicon A1 A2 A3 A4 x A4 y Amplification efficiency 95 a 98 93 10 0 95 Average efficiency 96.2 Genotype 1b Amplicon A1 x A1 y A2 A3 A4 x A4 y Amplification ... (see additional file 2: HCVMethodPaperTable2.xls) list amplification and sequencing primers for genotypes 1a and 1b. Table 3 (see additional file...

Ngày tải lên: 19/06/2014, 08:20

... not for citation purposes) Virology Journal Open Access Methodology A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame Ermei Yao 1 , ... efficient amplification. The HCV ORF was therefore partitioned into amplicons 1, 2, 3, 4x and 4y for genotype 1a and amplicons 1x, 1y, 2, 3, 4x...

Ngày tải lên: 20/06/2014, 04:20

... 5¢-GTTCACCACCAGAAGCT GGTGTTCTTC GCTGAA GACGT GGGTTCT AACAAG GGTGCT-3¢;Abc, 5¢-CACAACGCCACCAACCATCAGA CCGATGATAGCACCCTTGTTAGAACCCAC-3¢;Ab- start, 5¢-GCGTAGGGTCGACATATGGACGCTGAATT CCGTCACG-3¢;Abstop, ... SEC- isolated Ab (1 40) and Ab(M1–40) are at least 97% pure. In the lanes containing Ab (1 42) and Ab(M1– 42), there were prominent bands at approximately 4 kDa and faint bands at appro...

Ngày tải lên: 18/02/2014, 13:20

... Proceedings of the 39th Annual Meeting of the Association for Computational Linguistics, pages 18 8 -19 5. Ferreira da Silva, J. and G. Pereira Lopes (19 99). A local maxima method and a fair dispersion ... here, the mutual rank ratio, is a nonparametric rank-based approach which appears to perform significantly better than the standard association metrics. The...

Ngày tải lên: 08/03/2014, 04:22

... serial or parallel and one-pass or iterate. In all, 4 different combinations are possible : parallel and one-pass parallel and iterate serial and one-pass serial and iterate 89 4. FACILITIES ... these data and the interpreter then uses them to process the input text. The decoder transforms the result into a digestable form for the user. 3.2 Data structure...

Ngày tải lên: 24/03/2014, 01:21

... Shaul Marcus, and Shaul Markovitch. 19 95. Contextual word similarity and estimation from sparse data. Computer, Speech and Language, 9 :12 3 15 2. Ido Dagan, Lillian Lee, and Fernando Pereira. 19 97. Similarity-based ... shown for each similarity measure. As for BC b and BC a , the results for the tuned and several other values for α are shown. Figure 1 shows t...

Ngày tải lên: 30/03/2014, 21:20

... Press, Cam- bridge, England, 19 85. [8] Perelra, F. C. N. and D. H. D. Warren. Definite clause grammars for language analysis - a survey of the formal- ism and a comparison with augmented transition ... choosing particular values for the fea- ture). In the analysis of a particular sentence most features have a unique value, and some features are not present at a...

Ngày tải lên: 31/03/2014, 17:20

... participation in the recruitment and retention of rural doctors: methodological and logistical considerations. Australian Journal of Rural Health 19 99, 7:206- 211 . 18 . Lapalla M, Brandt E, Barker A, ... perspective, even disease or programme specific initiatives, such as the Global Alli- ance for Vaccination and Immunization (GAVI), the Global Fund to Fight AIDS, Tuberculo...

Ngày tải lên: 18/06/2014, 17:20

... 0.26 ↓ 10 0 10 1 10 2 10 3 10 4 APC -A: CD8 APC -A 10 0 10 1 10 2 10 3 10 4 PE -A: MelA-Pentamer PE -A 0.034 0.076 42.857 .1 10 0 10 1 10 2 10 3 10 4 APC -A: CD8 APC -A 10 0 10 1 10 2 10 3 10 4 PE -A: ... including organizational and scientific aspects, data analysis and interpretation as well as manuscript writing and approval. All author s read...

Ngày tải lên: 18/06/2014, 22:20

... from 11 to 20. Therefore, for block 1 11 n = 14 and for block 12 –20 n = 13 , 11 , 11 , 10 , 9, 8, 6, 3 and 1 respectively. For each box plot, the whiskers represent maximum and minimum values, the ... the top and bottom of the box represent the 75th and 25th quartiles and the horizontal line in the box represent the median. 0 5 10 15 20 25 30...

Ngày tải lên: 19/06/2014, 08:20