Báo cáo sinh học: " Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneratio" potx
Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... CCGGTG
GTcGAcTTTGGTTtcACGCCC
SalI
E273Q ACGTACTGCTgTCCGGAA
AGtACtCCATGGCCGC
ScaI
D353N TCCAATCCGTtAGTC
TGgCCaTAGAACGCGC
MscI
E356Q GGAGAAT
GGTgCCAGGGTACATTTcCAATCCGTCA
KpnI
D372N AATACAT
CTTaAGGCCGTCGTtGTCGGTGTGG
AflII
D373N ... described in Materials and methods.
Lower c ase letters indicate the nucleotide m utations. To facilitat e the
selection of mutant clones, silent mutations were...
Tài liệu Báo cáo khoa học: Differentiation stage-dependent preferred uptake of basolateral (systemic) glutamine into Caco-2 cells results in its accumulation in proteins with a role in cell–cell interaction pptx
... (NUTRIM), Department of Human Biology,
Maastricht University, the Netherlands
Glutamine has an important function in the small
intestine with respect to maintaining the gut epithelial
barrier in ... intestinal cells obtain glutamine through exo-
genous and endogenous routes. The exogenous gluta-
mine comes from uptake of the amino acid itself or
of glutamine-containing pepti...
Tài liệu Báo cáo khoa học: Oxygen tension regulates the expression of a group of procollagen hydroxylases docx
... lysylhydroxylase II
Sense:
5¢-GCACATTGGGAAACGCTA-3¢
Antisense:
5¢-AATTTTGCATTTGTGATC-3¢
Rat (gi: 6755107) lysylhydroxylase II
Sense:
5¢-CCTGTTGTGCACATTGGG-3¢
Antisense:
5¢-AATTTTGCATTTGTGATC-3¢
Mouse (gi: 424103) ... II
Sense:
5¢-CGGGATCCTGCAGGCAGAATTCTTCA-3¢
Antisense:
5¢-GGAATTCCCAGTCTGTGTTCAACCG-3¢
Rat (gi: 6754969) prolylhydroxylase alpha II
Sense:
5¢-CGGGATCCTGCAGGCAGAATTCTTCA-3¢
Antisen...
Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... 5¢-AT
TAATGGCATGCTTTACCCGT-3¢.UsingthePCR
products of agmatinase with the GfiAandC T substi-
tutions in the coding and noncoding strands, respectively,
and using the 5¢ and 3¢ primers mentioned above, the full
length agmatinase ... same
region of the arginases (Fig. 4). Since the arginase loop
contains residues that interacts with the a-carboxylate
group of the substrate arginin...
Báo cáo khoa học: Phaiodotoxin, a novel structural class of insect-toxin isolated from the venom of the Mexican scorpion Anuroctonus phaiodactylus pdf
... phaiodotoxin supports the
proposition of Froy and Gurevitz [38], that the C-terminal
tail of the S cTXs are playing an important role in the
biological activity of these toxins, and should constitute ... insect-toxin Bj’xtrIT from Butothus
judaicus [35]. The insect toxin 1 from Androctonus australis
(AshIT1) has 71 amino acids [36]. These t wo last toxins were
described as i...
Báo cáo khoa học: Unravelling the functional interaction structure of a cellular network from temporal slope information of experimental data docx
... f
ij
> 0 then we interpret that
the node j activates the node i by increasing the net rate
of x
i
and if f
ij
< 0 then the node j inhibits the node i.
We note that the dynamics of the system in ... results in Figs 7 and 8).
Sign equations for identification of the functional
interaction structure
In order to identify the true interaction structure
through the sign of f...
Báo cáo khoa học: O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, specifically expressed in the olfactory epithelium in a zone-specific manner doc
... guanine nucleotide releasing protein ND – Rat IGF binding protein 5 ND –
Rat FGF receptor activating protein 1 ND –
Rat PKC delta-binding protein ND –
Rat cellular retinoyl-binding protein ND ... catalyzes the first step of the b-oxidation in
mitochondria. It is also possible that O-MACS is involved in
the clearance of excess fatty acids taken into the supporting
cells and cells...
Báo cáo Y học: An active site homology model of phenylalanine ammonia-lyase from Petroselinum crispum docx
... substrate-free state of the enzyme as its
conjugated acid (Fig. 7A,F), from which the amino group
of the substrate can abstract a proton during entering into
the active site (Fig. 7C). Interaction of the ... conclude that the mechanism
involving the attack of MIO at the aromatic portion of the
substrates is more consistent with the experimental data and
modelling studies than...
Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx
... 5¢-CACCCAAGCTT
GCCACCATGGAGGTTCAGCTGCAGCAGTCTG-3¢; primer 7, 5¢-GGT
GGAGGAGGTTCTGATGTTTTGATGACCCAAACTCCAC-3¢; primer 8,
5¢-CGAAT
GCGGCCGCCCGTTTGATTTCCAGCTTGGTGC-3¢; primer 9,
5¢-GGTGGAGGAGGTTCTGATGTTGTTCTGACCCAAACTCCACTC-3¢;
primer ... 5¢-CGTCTCCTCAGGGGGTGGTGGTTCTGGAGGTGGAG
GATCCGGTGGAGGAGGTTCT-3¢; primer 14, 5¢-CGTCTCCTCA
GGGGGTGGTGGTTCTGGAGGTGGAGGATCCGGTGGAGGAGG
TTCT-3¢. In all primers, u...
Báo cáo khoa học: Multidrug efflux pumps: The structures of prokaryotic ATP-binding cassette transporter efflux pumps and implications for our understanding of eukaryotic P-glycoproteins and homologues ppt
... how is the extent of
domain separation controlled? What are the attractive
forces that bring the domains back into contact – is it
possible that electrostatic attraction across a solvent-
filled ... proximity to the ATP-binding
sites [85,86]. The aromatic clusters within the ICLs of
CFTR are almost certainly involved in effecting inter-
domain communication between the NBDs and
TMDs,...
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