... Microsequencing analysis of the MREa-binding proteins. (A) The N-terminal sequence of the 82-kDa band was identical to amino-acid positions 6–16 of the Ku-80 protein. (B) The 70-kDa band did not ... another band of 82 kDa when the MREa-protein band from the EMSA was excised from the native gel and resolved by SDS/PAGE (unpublished data). We purified the MREa-binding...
Ngày tải lên: 17/03/2014, 23:20
... but not the control protein 6xHis–RACK1. The figure also shows the equal loading and input controls of the tested proteins. Fig. 1. Alignment of Ki-1 ⁄ 57 and CGI-55 and prediction of putative arginine ... chromo-helicase DNA-binding domain protein 3 and with the adaptor ⁄ signaling protein receptor of activated kinase 1 in the nucleus. Among the identified prote...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: Active site residues governing substrate selectivity and polyketide chain length in aloesone synthase docx
... maintains almost iden- tical CoA binding site and the catalytic triad of Cys164, His303, and Asn336 (numbering in M. sativa CHS) (Fig. 2). Furthermore, most of the active site residues including ... cavity. On the other hand, the residue Thr338 (ALS) and Val338 (OKS) located in proximity of the catalytic Cys164 at the ‘ceiling’ of the active site cavity guides...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo khoa học: "An Ensemble Model that Combines Syntactic and Semantic Clustering for Discriminative Dependency Parsing" pptx
... cluster labels, and hence some of the cluster-based features. In a fu- ture work, we plan to take the union of all of the feature sets and train a joint discriminative parsing model. The ensemble ... semantic in- formation into the model (in contrast with the use of POS tags in the baseline) and help alleviate the sparse data problem for complex features t...
Ngày tải lên: 17/03/2014, 00:20
Báo cáo Y học: Importin a binds to an unusual bipartite nuclear localization signal in the heterogeneous ribonucleoprotein type I pptx
... domain binds strongly to importin a and the bind- ing is diminished or eliminated even if only one of the two basic stretches are mutated. The affinity of the importin- targeting sequence interaction ... karyopherins [8,9]. Importins a contain the NLS-binding site and importins b are responsible for the docking of the importin–substrate complex to the cytoplasmic side...
Ngày tải lên: 18/03/2014, 01:20
Báo cáo Y học: DAP kinase activity is critical for C2-ceramide-induced apoptosis in PC12 cells ppt
... of the human DAP kinase mutant proteins u se d in these ex pe rimen ts. The DCaM-DK mutant lack s 47 a mino acid residues (pro line 277 to leucine 323) containing the calmodulin-binding domain. ... DAP kinase, death-associated protein kinase; MLC kinase, myosin light chain protein kinase; CaM kinase, Ca 2+ /cal- modulin-dependent protein kinase; ZIP kinase, zipper-interacting protei...
Ngày tải lên: 24/03/2014, 00:21
Báo cáo khoa học: A nonribosomal peptide synthetase (Pes1) confers protection against oxidative stress in Aspergillus fumigatus ppt
... coverage of the N-ter- minal and C-terminal domains, respectively, of Pes1). Furthermore, using MALDI LIFT-TOF ⁄ TOF MS, the sequence of four peptides derived from Pes1 was deter- mined. Deletion of ... upregulation of pes1 and diminution of the latter, possibly implying a difference in functionality and bringing into question the classification of pes1 as a putat...
Ngày tải lên: 30/03/2014, 10:20
Báo cáo khoa học: "A SENTENCE ANALYSIS METHOD FOR A JAPANESE BOOK READING MACHINE FOR THE BLIND" pptx
... (b') and (b3) in Table 4 indicates the effectiveness of the sentence analysis method. The score 99.0% in Table 3 indicates the efficiency of the sentence analysis method in the book reading ... combination of phrases ) by examining the phrases in network structure. After organizing the sentence, the information for speech synthesis is then organiz...
Ngày tải lên: 31/03/2014, 17:20
Báo cáo khoa học: Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx
... bc-crystallin fold. The residues shown in grey indicate the side chains and backbone sites that are involved in calcium binding [24]. The residues in the conserved tyrosine corners are in bold [24]. ... domain folds, spheru- lin 3a and NKA48 contain only a single domain fold. The stability of these two proteins is highly dependent on calcium binding [25]. The typical...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo y học: "A prospective observational study of the relationship of critical illness associated hyperglycaemia in medical ICU patients and subsequent development of type 2 diabetes"
... resistance and dysfunction of beta cells. Insulin resistance is present in the acutely ill [13,15,18,28] in different intensity, but the factors determining the extent of insulin resistance are not ... 14:R130 http://ccforum.com/content/14/4/R130 Page 6 of 8 of beds available on cardiology wards and in the coronary care unit during the inclusion years. We used OGTT...
Ngày tải lên: 25/10/2012, 10:02