methods in molecular biology, v 303 nanobiotechnology protocols, 2005, p 242

... track specific proteins tagged with complemen- tary fluorescent probes. 3.1.3. In Vivo Applications Several reports have appeared utilizing quantum dots in vivo. This work is typically accomplished ... new opportunities in multiplexed assay development and greatly facilitate the study of cell/cell interactions and other complex phenotypes in mixed cell populations. 4. Future Perspecti...

Ngày tải lên: 04/06/2014, 15:14

... the employed protein dissolved in an appropriate solvent (35,36). Exemplarily, lyophilized Sfl is dissolved in HFIP and cast on a surface like polyethylene (PE). After evaporation of HFIP, the ... chimera combining a spider silk domain and an elastin peptide or a dentin matrix protein have been successfully engineered (43,45). Chimeric silk proteins are capable of providing a wide var...

Ngày tải lên: 04/06/2014, 15:06

... be marked properly as “mycoplasma positive” to prevent a mix up of ampoules containing cured or infected cells. After successful cure, these mycoplasma-positive ampoules should be removed and the ... or matrix- binding extraction methods. To confirm the error-free preparation of the sample and PCR run, appropriate control reactions have to be included in the PCR. These comprise internal co...

Ngày tải lên: 08/04/2014, 12:50

... F-box binding protein (56). The SCF complex is consti- tutively active throughout the cell cycle. Substrate phosphorylation drives capture by specific F-box proteins, which include Cdc4 for phosphorylated ... (73). Cdc28-dependent phosphory- lation inhibits Cdh1/Hct1, preventing it from binding to the APC before anaphase is complete (74,75). This phosphorylation is removed by Cdc14, a phospha...

Ngày tải lên: 08/04/2014, 12:50

... Microinjection 99 99 From: Methods in Molecular Biology, Vol. 127: Molecular Methods in Developmental Biology: Xenopus and Zebrafish Edited by: M. Guille © Humana Press Inc., Totowa, NJ 9 Preparation ... make specific null mutants in Xenopus laevis has recently been overcome, in part, by the overexpression of ingenious vari- ants of the endogenous proteins under study. The...

Ngày tải lên: 11/04/2014, 09:52