... Sulfated and nonsulfated glycosamino-glycans (GAGs) from the supernatant were estimated by the Whiteman Alcian blue binding technique [28,29], usingchondroitin sulfate as standard. The assay was ... EDTA-SM than for WSM (Fig. 2). The mainpeak from each separation, indicated by an asterisk in Fig. 2, was collected and submitted to amino acid analysis.Amino acid compositions In accordance ... analysis and calcium measurements of the water-soluble matrix, the EDTA-soluble matrix and the EDTA-insolublematrix of Pinctada maxima nacre. Sulfated and nonsulfated glycosaminoglycans from the...