t cell protocols

... B cells, but still retains a capacity to form DC (9). It is not until the c-kit – CD44 – CD25 + stage that the precursors are completely com- mitted to T lineage development (9). Both c-kit – CD44 – CD25 + and ... interesting (82). 4. Mature T Cell Differentiation The mechanisms of mature T cell survival in the absence of antigen and the functional differentiation of T cells upon...

Ngày tải lên: 11/04/2014, 10:34

... CACCATATCCCGCTTGAGTT Real-time PCR Zfgata3-F GCTTCTTCCTCCTCGCTGTC Real-time PCR Zfgata3-R TGCACTCTTTGTCTTCCTGTCG Real-time PCR Zfstat6-F CGGTAGTCAGGAAATCAATGC Real-time PCR Zfstat6-R ATCTGTCCAATAGTCTCGTAGG ... (5¢-to3¢) Used for Zfstat6-F1 AGTGAGATGGATACAGGTGCTAAAC Initial PCR Zfstat6-R1 TCTGGACCTCAGACATGAACTTACT Initial PCR Zfstat6-F2 TGTCAGTCCTCTTTAATGCT Initial PCR Zfstat6-R2 AATGGTATCCTGT...

Ngày tải lên: 16/02/2014, 09:20

... less than 5% inhibitory activity at three different concentrations studied. These peptides were also tested for their toxicity using the MTT assay [17]. All the four peptides tested in the study ... inhibitory activities of the peptides, the structures of the cyclic peptides were determined by NMR. The results proved that the stable b-turn conformation exists in the cyclic peptides. The b-tur...

Ngày tải lên: 19/02/2014, 13:20

... but the data concern- ing other T- cell receptor (TCR)⁄ CD3 constituents remains contradictory [6,7]. In addition, the partition- ing or recruitment of CD3e to lipid rafts after TCR stimulation ... membrane fraction contained cholesterol at a level more than twofold that of the unbound fraction, a finding consistent with its higher C ⁄ P ratio. It is noteworthy that the PS ⁄ PI intensity ra...

Ngày tải lên: 20/02/2014, 03:20

... 3BP2-mediated NFAT activation in response to anti-CD3 stimulation in 3BP2-transfect- ed cells. Furthermore, SHP-1 also inhibited the consti- tutive NFAT activation in 3BP2-transfected cells but not the ... detected in the immunoprecipitant from cells transfected with cata- lytically activated Lck but not in the immunoprecipi- tant from the cells transfected with wild-type Lck SHP-1 and 3BP2 i...

Ngày tải lên: 07/03/2014, 12:20

... 5¢-GGTTATCATA TAAAGATCTCAAATTACCC-3¢, for the second PCR the primers were: 5¢ primer, 5¢-GGGTAATTTGAGATC TTTATATGATAACC-3¢ and 3¢ primer, 5¢-CGCGCGG GATCCTTAGTGATGGTGATGGTGATGGGTGACC GGTTTTTTGGTAGGTGAAC-3¢.ThethirdPCRwas carried ... region encoding the mature protein (5 ¢ primer, 5¢-CATGCCATGGCCAGTAGTCAGCC TGACCCTACT CCAG-3¢;3¢ primer, 5¢-CGCGCGGGATCCTTAGTG ATGGTGATGGTGATGGGTGACCGGTTTTTTGG 40...

Ngày tải lên: 07/03/2014, 16:20

... culminates in T- cell activation. Therefore, phosphorylation of ITAM tyrosines is an absolute requirement for the TCR-mediated trigger of T- cell activation [2,7]. Probing the TCR with different stimuli ... location in the TCR, with the ITAM closest to the N-terminus/membrane referred to as 1, the next closest as 2, and the farthest as 3. The two tyrosines within each ITAM are then furt...

Ngày tải lên: 08/03/2014, 02:20

... relapse of ATL in postallogeneic stem cell transplantation patients. The expression of Tax by the host cell targets them for attack by CTL, resulting in the elimination of the infected cell [32]. ... unpulsed T2 -A2 cells as target cells, specific cytotoxic activity was evaluated by FCM assay of cell- mediated cytotoxicity. All tests were carried out in triplicate at effector : target...

Ngày tải lên: 14/03/2014, 23:20

... 5¢-CACCCAAGCTT GCCACCATGGAGGTTCAGCTGCAGCAGTCTG-3¢; primer 7, 5¢-GGT GGAGGAGGTTCTGATGTTTTGATGACCCAAACTCCAC-3¢; primer 8, 5¢-CGAAT GCGGCCGCCCGTTTGATTTCCAGCTTGGTGC-3¢; primer 9, 5¢-GGTGGAGGAGGTTCTGATGTTGTTCTGACCCAAACTCCACTC-3¢; primer ... 5¢-CGTCTCCTCAGGGGGTGGTGGTTCTGGAGGTGGAG GATCCGGTGGAGGAGGTTCT-3¢; primer 14, 5¢-CGTCTCCTCA GGGGGTGGTGGTTCTGGAGGTGGAGGATCCGGTGGAGGAGG TTCT-3¢. In all primers, und...

Ngày tải lên: 16/03/2014, 14:20

... stimulation was also investigated. This was the tyrosine phosphorylation of STAT3/STAT5, monitored through binding of anti-(phospho-tyr-STAT3/STAT5) Ig. As Fig. 5B shows, stimulation of the Kit225K6 ... nonphosphorylated and phosphor- ylated Tyr moieties on S TAT3/STAT5, respectively, without appreciable cross-reaction with other Tyr-phosphorylated STATs. After washing, cells were incubated w...

Ngày tải lên: 17/03/2014, 23:20