... unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Keywords: Aged; Elderly nutrition; Food consumption; Homes for the aged; Malnutrition; ... as identied by BMI and MAN, proved to be associated with mobility, frequency of visits and nancial contribution by the elderly to the ILPI. e very old people with reduce...
Ngày tải lên: 05/03/2014, 21:20
... purified an RNA pol II holoenzyme complex con- taining multiple DNA replication and repair proteins, including DNA polymerase (Pol) e, Ku, Rad51, RPA and RFC. This finding was confirmed and extended by ... lgÆmL )1 ethidium bromide (EtBr) to exclude interac- tions mediated by DNA. Hyperphosphorylated RNA pol IIO is indica- ted with a solid arrow and hypophosphorylated RNA pol IIA...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Investigating RNA polymerase II carboxyl-terminal domain (CTD) phosphorylation ˆ Benoıt Palancade and Olivier Bensaude pptx
... and embryonic development. Keywords: RNA polymerase II; CTD-phosphorylation; CTD-kinase; CTD-phosphatase; transcription; mRNA processing. The C-terminal domain (CTD) of RNA polymerase II’s (RNAP ... early studies and designated RNAP IIA and RNAP IIO [20]. They differ in the phosphorylation status of their largest subunit (Rpb1), respectively, designa- ted as IIa and IIo forms...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Distinct parts of minichromosome maintenance protein 2 associate with histone H3/H4 and RNA polymerase II holoenzyme pptx
... FLAG-tagged MCM2 deletion mutants were individually transfected in 293 cells and expressed for 36–48 h. Whole cell extracts were prepared as described in Materials and methods and 90 lg were loaded per ... This plasmid and all its derivatives encode MDYKDDDDK LAAANSAESSESFT followed by different MCM2 frag- ments. pFLAG-MCM2(1–197), pFLAG-MCM2(1–247), and pFLAG-MCM2(1–511) were gener...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot
... selectively hydrolyzes the RNA strand of the RNA: DNA hybrid formed during synthesis of the minus (–) strand DNA that uses (+)-strand RNA as template [2,3]. HIV-1 RNase H, similar to all the RNase Hs and together with transposases, ... some AQ derivatives bind noncovalently to double-stranded (ds)DNA [9] and given that the reaction substrates used in our biochemical assays were RNA:...
Ngày tải lên: 22/03/2014, 16:20
Báo cáo khoa học: Aptamers toEscherichia colicore RNA polymerase that sense its interaction with rifampicin, r-subunit and GreB ppt
... specific and nonspecific contacts with double and single stranded (ss) DNA, the RNA/ DNA hybrid and nascent RNA. Recent advances in structural studies of bacterial and yeast RNAPs [ 4–8] made it possible ... channel a ccommodates t he RNA/ DNA hybrid, duplex DNA downstream from the hybrid and RNA behind the hybrid. The 8-bp-long DNA /RNA hybrid is lodged between t he catalytic...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: Mode of action of the microbial metabolite GE23077, a novel potent and selective inhibitor of bacterial RNA polymerase docx
... reduced when it is added a fter the formation of the RNAP–DNA complex [11]; and rifampicin, which, conversely, binds and inhibits RNAP equally well if added when t he enzyme is already bound to DNA ... molecule. Materials and methods Enzymes and antibiotics Purified E. coli holo and core RNAP, E. coli DNA polymerase (DNAP) and wheat germ RNAP II w ere from Epicentre T echnologies...
Ngày tải lên: 30/03/2014, 15:20
Báo cáo y học: "Prevalence of Overactive Bladder, its Under-Diagnosis, and Risk Factors in a Male Urologic Veterans Population"
... increased with age. OAB was not associated with BMI, smoking, race, diabetes, CHF, and COPD. Conclusions: The pre- valence of OAB in this population is under-diagnosed and under-treated. Key words: ... included urinary frequency (2 questions), urgency (2), nocturia (1), incontinence (2) and emptying (1). The questionnaire also included medical and surgical history, demographic...
Ngày tải lên: 25/10/2012, 11:35
Báo cáo y học: "A review of anatomical and mechanical factors affecting vertebral body integrit
... trunk in individuals with osteoporosis. John D Wark (PhD), Professor of Medicine, Endocrinologist, is Head of the Bone and Mineral Service at Royal Melbourne Hospital and Broadmeadows Osteoporosis ... AF, Adams MA, Dolan P. Sudden and unexpected loading generates high forces on the lumbar spine. Spine. 2000 25(7):842-852. 52. Martini FH. Fundamentals of Anatomy and Physiology,...
Ngày tải lên: 03/11/2012, 09:49
Significant substitutive figures of speech – linguistic functions and pedagogical implications part 1
Ngày tải lên: 07/11/2012, 14:24