coli rna polymerase on single stranded and double stranded dna templates

... incorporation into DNA, and is reduced further on formation of a duplex The latter distinction, duplex vs single- stranded, is exploited to monitor regions of the DNA melted open by RNA polymerase ... considered that interactions with DNA and RNA might limit the range of sliding For example, base pairing between DNA and RNA and interactions between RNA and polymerase would keep the polymerase from ... ternary complex (DNA RNAP RNA) is diffuse and is pushed upstream at higher exonuclease concentrations The quaternary complex (DNA RNAP RNA NTP) is more resistant to the processive exonuclease, as...

Ngày tải lên: 11/04/2014, 10:30

... sections 2– 21 and the regularizer constant c adjusted for optimal f -score on section 24 and evaluated on sentences of length less than 100 in section 23 trained the n-best parser on sections ... heads and their ancestors’ categories An additional parameter controls whether the feature is specialized to embedded or non-embedded clauses, which roughly corresponds to Emonds’ “nonroot” and ... but one of the parsing decisions in second-best parse must be optimal, and the one suboptimal decision must be the second-best choice at that choice point Further, the nth-best parse can only...

Ngày tải lên: 08/03/2014, 04:22

... through the association of three dimers [10] (PDB ID 2ixd) Dimerization is achieved through interaction between the b8-strand of one monomer and the b6- and b7-strands of the other monomer (B) Surface ... interactions with residues that occupy positions 140 and 42 are indicated (C) Position 140 The native Arg residue (carbon atoms in yellow) and the mutations Ala (carbon atoms in brown) and Glu (carbon ... genomic DNA by PCR and cloned into the pRSETA expression vector for recombinant protein production in E coli Purification was achieved in two steps, using ion-exchange and size-exclusion chromatography...

Ngày tải lên: 15/03/2014, 11:20

... understanding their physiological functions Materials and methods Materials Mouse monoclonal anti-DC-SIGN (clone 120507), mouse monoclonal anti-DC-SIGNR (120604), polyclonal goat FEBS Journal 277 ... Luo Q, Levroney E, Trowsdale J, Montaner LJ, Doms RW, Weissman D et al (2002) Constitutive and induced expression of DC-SIGN on dendritic cell and macrophage subpopulations in situ and in vitro ... expression of DC-SIGN-related lectins on CHO cells, · 106 cells in NaCl ⁄ Pi ⁄ BSA were incubated with 10 lgÆmL)1 of primary antibodies (monoclonal antiDC-SIGN, monoclonal anti-DC-SIGNR, monoclonal...

Ngày tải lên: 15/03/2014, 23:20

... Jinjoo Park1, and Junsin Yi*1 School of Information and Communication Engineering, Sungkyunkwan University, 300 Cheoncheon-dong, Jangan-gu, Suwon, 440-746, South Korea Photovoltaic Research Center, ... with SiH4 and H2 gases The dilution ratios, R = H2/SiH4, are and 0.8 for i-type a-Si:H and p-type a-SiC:H, respectively The substrate temperature and deposition pressure are kept at 200°C and 0.3 ... microcrystalline silicon p layer J App Phys 1999, 86:6879-6889 [5] Lee JC, Dutta V, Yoo J, Yi J, Song J, Yoon KH: Superstrate p-i-n a-Si:H solar cells on textured ZnO:Al front transparent conduction oxide...

Ngày tải lên: 20/06/2014, 20:20

... n-norms on X We call these n-norms as α-n-norms on X corresponding to the fuzzy n-norm on X Definition 2.3 Let X be any non-empty set and fuzzy sets on X For U, V ∈ (X) the set of all (X) and λ ... introduced the definitions of fuzzy norms on a linear space Cheng and Mordeson [7] and Bag and Samanta [8] introduced a concept of fuzzy norm on a linear space The concept of fuzzy n-normed linear spaces ... spaces In this section, we define the concepts of 2-fuzzy n-normed linear spaces and α-n-norms on the set of all fuzzy sets of a non-empty set Definition 3.1 Let X be a non-empty and fuzzy sets in...

Ngày tải lên: 20/06/2014, 21:20

... Jinjoo Park1, and Junsin Yi*1 School of Information and Communication Engineering, Sungkyunkwan University, 300 Cheoncheon-dong, Jangan-gu, Suwon, 440-746, South Korea Photovoltaic Research Center, ... with SiH4 and H2 gases The dilution ratios, R = H2/SiH4, are and 0.8 for i-type a-Si:H and p-type a-SiC:H, respectively The substrate temperature and deposition pressure are kept at 200°C and 0.3 ... microcrystalline silicon p layer J App Phys 1999, 86:6879-6889 [5] Lee JC, Dutta V, Yoo J, Yi J, Song J, Yoon KH: Superstrate p-i-n a-Si:H solar cells on textured ZnO:Al front transparent conduction oxide...

Ngày tải lên: 21/06/2014, 17:20

... Undersecretary for International Affairs Tim Adams, White House economic spokesman Tony Fratto, and Chairman of the Council of Economic Advisers Ed Lazear for educating me on how Washington works and interacts ... committed to developing and marketing print and electronic products and services for our customers’ professional and personal knowledge and understanding The Wiley Trading series features books ... intervention On this, I have relied on Andy Busch for expert analysis for more than a decade He reads the tea leaves of international markets with a keen understanding that past is prologue and humans...

Ngày tải lên: 09/08/2014, 23:20

... Hong Kong and China announce detection of a SARS-like virus in the masked palm civet and racoon-dog These and other wild animals are traditionally consumed as delicacies and sold for human consumption ... Beijing, Taiwan (China), and Singapore have developed SARS following travel to Hong Kong —WHO recommends that persons traveling to Hong Kong and Guangdong Province consider postponing all but essential ... Iraq and UN weapon inspections led by Hans Blix and Mohamed ElBaradei The UN and Iraq were engaged in a game of hide -and- seek with banned weapons and then Blix would report back to the UN on the...

Ngày tải lên: 09/08/2014, 23:20

... farms and wet markets in Hong Kong Not long after, Hong Kong reported human infections with the virus, with 18 cases and six fatalities This is the first reported incidence of human infection with ... influenza” in 1957, and “Hong Kong influenza” in 1968 The 1918 pandemic killed an estimated 40 to 50 million people worldwide That pandemic, which was exceptional, is considered one of the deadliest ... Estimates based on a more virulent virus, closer to the one seen in 1918, have been made and are much higher However, the 1918 pandemic was considered exceptional Economic and social disruption will...

Ngày tải lên: 09/08/2014, 23:20

... a look at the wonderful diagram of the region that appeared in the Wall Street Journal on September 30, 2006 (Figure 6.25) It shows the 745 oil rigs and platforms abandoned along the Gulf of ... weather on 16 September This system became a depression just east of the Turks and Caicos Islands late on 17 September, which moved westward and became a tropical storm the following afternoon Maximum ... the Bush administration to spend $110 billion to rebuild the region On the big indexes, the Dow dropped to its low for the month as Hurricane Katrina approached and then made landfall (see Figure...

Ngày tải lên: 09/08/2014, 23:20

... international agreement from big emitters to go along with reductions We must filter these issues through a political lens to really understand what’s at stake INTERNATIONAL AND NATIONAL NONAGREEMENTS ... the solution and what is fair? It would be wonderful if this was exactly like the ozone layer and we could get broad agreement for an international accord (Montreal) that limited production of chlorofluorocarbons ... United States, the European Union (25 countries), China, Russia, Japan, and India, according to the Organization for Economic Cooperation and Development (OECD)’s International Energy Agency (IEA)...

Ngày tải lên: 09/08/2014, 23:20

... same The coordinated attacks hit London during rush hour, blowing up three London Underground trains and one double- decker bus The explosions killed 52 passengers, and 700 were injured (four suicide ... elections of 1994 were so important and laid the groundwork for the travails of both Gingrich and Clinton that ensued as one lost his job and one got impeached ARGENTINE 2001 ELECTIONS AND CRISIS ... daylong disruption of London’s transportation and mobile telecommunication infrastructure The European Central Bank’s Jean-Claude Trichet, the Bank of England’s Mervyn King, and the Federal Reserve’s...

Ngày tải lên: 09/08/2014, 23:20

... Volcanoes, and Their Impact on Humankind New York: W H Freeman & Company, 1998 Marland, G., T A Boden, and R J Andres “Global, Regional, and National CO2 Emissions.” In Trends: A Compendium of Data on ... Communications industry, 22 Communist Party, 206 Conflicts, see War Construction industry, 134 Consumer discretionary sector, 80 Consumer price index (CPI), 212, 214 Contract with America, 173 Coronavirus, ... indication of his view of Bush, Conyers released a report entitled, “The Constitution in Crisis: The Downing Street Minutes and Deception, Manipulation, Torture, Retributions, and Cover-ups in the Iraq...

Ngày tải lên: 09/08/2014, 23:20

... USA 20 1933 Dr Michael Bukrinsky Ed Board George Washington Univ Washington DC USA 25 4913 Dr Dong-yan Jin Ed Board Hong Kong U Hong Kong China 22 2402 Dr Klaus Strebel Ed Board NIH Bethesda ... others for mentoring contributions during a defined period (e.g 1991 to 1995) Of course, comparisons are meaningful only when done amongst appropriate peer groups A focus on using the HI of previous ... Greg Towers Ed Board University College London UK 17 1392 Dr Graham Taylor Ed Board Imperial College London UK 15 1567 Dr Eric Cohen Ed Board Univ Montreal Montreal Canada 27 3221 Dr William Hall...

Ngày tải lên: 13/08/2014, 05:21

... Organisation, and therefore ICUs should use the DDD to make national and international comparisons of their antibiotic use Conclusions Disturbingly, ICUs have little in reserve to control multidrug ... consumption in German intensive care units from 2001 to 2008 DDD, defined daily doses; pd, patient-days; 1&2GC, first-generation and second-generation cephalosporins; 3&4GC, third-generation and ... also contribute to the selection of ESBLs, to the persistence of pre- dominant ESBL clones and to the probable dissemination of conjugative plasmids among strains Limiting administration of these...

Ngày tải lên: 13/08/2014, 20:22

... investigated Currently, the Acute Physiology and Chronic Health Evaluation II (APACHE-II) score is one of the most common models used to evaluate ICU patients’ condition and predict their outcomes [23] The ... upregulation of cardiac brain natriuretic peptide at the transcriptional and translational levels by proinflammatory cytokines and by conditioned medium derived from mixed lymphocyte reactions via ... cell and heart rate, and had lower eGFR and blood pressure on admission in the ICU as compared with survivors (Table 1) Predictors of ICU mortality Univariate logistic regression analysis demonstrated...

Ngày tải lên: 14/08/2014, 07:21

... Generation of novel interaction candidates for PAK1 Figure peaks (Pks) methods as a function of p value expression Comparison of the FDRs for the Pearson correlation coefficient (Prs) and Comparison ... expression peaks (Pks) methods Comparison of sensitivity for the Pearson correlation coefficient (Prs) and Comparison of sensitivity for the Pearson correlation coefficient (Prs) and expression peaks ... Non-synchronized HeLa cells Non-synchronized HeLa cells The number of complexes with a best p value equal to or lower than the corresponding one on the x-axis is plotted for three non-synchronized...

Ngày tải lên: 14/08/2014, 08:20

Ngày tải lên: 25/11/2014, 00:34

... training Certification and education levels of professionals Hours of employee training completed Labor contract length Learning and Growth Dimenstion 364 Corporate Profitability and Shareholder Value ... types of professional associations that managers join to share ideas and continue the development of their personal and managerial skills Economic Value Added (EVA®) 365 ECONOMIC VALUE ADDED ... sales or by any combinations of alternatives and Exhibit 18.2 shows complete details of the relationship of ROI to the underlying ratios—margin and turnover and their components This will help identify...

Ngày tải lên: 06/04/2015, 18:04