receptor signal transduction protocols, 2nd

... solubilized receptors, receptors in tissue slices (receptor autoradiography), or receptors in intact animals. From: Methods in Molecular Biology, vol. 259, Receptor Signal Transduction Protocols, 2nd ... detection of G-protein-coupled receptor (GPCR) mRNAs using Northern blotting or ISH. From: Methods in Molecular Biology, vol. 259, Receptor Signal Transduction Protocols,...

Ngày tải lên: 11/04/2014, 10:25

... A, adenosme receptor and the rat A3 adenosine receptor to examine the structural requirements for ligand binding by adenosine receptors. The two wild-type receptors, as well as receptor chimeras ... 4. 7.7 2. Chimeric Receptors The vast majority of chimeric receptors employed to study ligand binding are constructed from two parent receptors that belong to the same receptor famil...

Ngày tải lên: 11/04/2014, 10:25

... (10). If nonreceptor binding occurs at similar concentrations to binding to the receptor and if the capacity of the nonreceptor sites is very large in comparison with the bona fide receptors, ... the receptor under investigation. However, the defi- nition of a receptor depends on function, which cannot be measured in a binding assay. Thus, the identification of any binding site a...

Ngày tải lên: 11/04/2014, 10:32

... Pro-apoptosis resulted mainly from the strong inhibition of the G-protein couple receptor signaling pathway. Moreover, the signal transduction initiated by ethanol-induced protein kinases phosphorylation ... association with a slight activation of the cell death receptor signaling. In addition, ethanol also phosphorylated protein kinases belonging to signal transduction pathw...

Ngày tải lên: 31/10/2012, 15:37

... sequestration for signalling dynamics Receptor desensitization is a relatively slow process and downstream signal transduction cascades are often in a quasi-steady-state with the receptor activity. ... [17], in which the rise in the signal is delayed but the dropping signal is transduced immediately. Finally, we analyse the effect of sequestration on a complex signal transducti...

Ngày tải lên: 19/02/2014, 07:20

... cell antigen receptor signaling M. Reth and Y. Kulathu Institute for Biology III and Max-Planck-Institute for Immunobiology, Freiburg, Germany. E-mail: reth@immunbio.mpg.de Signal transduction ... activation state of Rho-family signalling pathways, for example by down-regulation of ROCK or Rho activation and up-regulating Rac activation. S1.6-2 Signal transduction via receptors for...

Ngày tải lên: 19/02/2014, 07:20

... and flotillin, but also some T-cell receptor (TCR) signalling molecules, including Lck, Fyn and LAT. In addition, Csk and PAG, inhibitory molecules of the TCR signalling cascade, are also contained ... contribute to the on ⁄ off switching of TCR signalling. In addition to signalling machinery molecules, PAG and Csk, which are known to be negative regulators of TCR signalling [26], were also...

Ngày tải lên: 20/02/2014, 03:20

... partners: signaling proteins, receptors and ion channels PKA, PKC, PP2B, MAGUKs (SAP97, post synaptic density (PSD)-95), AC5, AMPA receptor, NMDA receptor, KCNQ2 channel, M1 muscarinic receptor, ... inhibit ERK5 signaling. (C) As cAMP levels decrease, the Epac1-mediated inhibition of ERK signaling is lost and mitogenic signaling favors cell growth. (D) mAKAP assembles an oxygen-sensitive s...

Ngày tải lên: 06/03/2014, 22:21

... acti- vin receptor- mediated signaling via the efficient recruit- ment of Smad2 to the receptor [21]. It is therefore evident that receptor endocytosis is an important early step in TGFb signal transduction. To ... the TGFb signaling pathway. Interconnection between TGF b signaling and receptor trafficking- regulation by small GTPases Endocytosis has long been considered a way of ter...

Ngày tải lên: 07/03/2014, 01:20

... the receptor and receptor kinases. Abbreviations BK, bradykinin; B x wt, wild-type B x bradykinin receptor; DAK, desArg10kallidin; GPCR, G protein-coupled receptor; GRK, G protein-coupled receptor ... of the cytosolic carboxyl termini of human B 1 and B 2 kinin receptors on receptor sequestration, ligand internalization, and signal transduction. J Biol Chem 273, 2617–2623. 17 Bla...

Ngày tải lên: 07/03/2014, 16:20