protein phosphatase protocols

protein phosphatase protocols

... of the protem phosphatase assay (5 mm m example), 5 = the fold that the protein phosphatase was diluted into the phosphatase assay. 3.4. Method D 3.4.1. Assay for Protein Phosphatase Using ... protem phosphatase assay (5 min m the example); 5 = the fold that protein phosphatase was diluted mto the phosphatase assay. 3.5. Method E 3.5.1. Continuous Spectrophotometric...

Ngày tải lên: 11/04/2014, 10:12

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Báo cáo khoa học: Neural retina leucine-zipper regulates the expression of Ppp2r5c, the regulatory subunit of protein phosphatase 2A, in photoreceptor development pdf

... slower-migrating DNA protein complexes in a dose-dependent manner (lanes 4 and 5), whereas the control GST protein alone did not form DNA protein complexes (lanes 2 and 3). The presence of Nrl in the protein DNA ... of protein phosphatase 2A. FEBS Lett 460, 462–466. 7 Zolnierowicz S, Csortos C, Bondor J, Verin A, Mumby MC & DePaoli-Roach AA (1994) Diversity in the regulatory B-...

Ngày tải lên: 06/03/2014, 02:20

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Báo cáo khoa học: The Saccharomyces cerevisiae orthologue of the human protein phosphatase 4 core regulatory subunit R2 confers resistance to the anticancer drug cisplatin pot

... methanesulphonate; Pph, Saccharomyces cerevisiae protein phosphatase; Ppp ⁄ PP, protein serine ⁄ threonine phosphatase in mammals and Drosophila; Ppp4c, protein phosphatase 4 catalytic subunit (also termed ... Cancer Res 64, 3940–3948. 2 Cohen PTW (2004) Overview of protein serine ⁄ threonine phosphatases. In Topics in Current Genetics: Protein Phosphatases (Arino J & Ale...

Ngày tải lên: 07/03/2014, 12:20

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Báo cáo khoa học: Identiﬁcation and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot

... (2001) Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling. Biochem. J. 353, 417–439. 2. Sontag, E. (2000) Protein phosphatase ... dependent on the opposing activities of kinases and phosphatases. Among the phosphatases, protein phosphatase 2A is a major serine/threonine protein phosphatase involved in...

Ngày tải lên: 08/03/2014, 08:20

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Báo cáo khoa học: Two conserved domains in regulatory B subunits mediate binding to the A subunit of protein phosphatase 2A pdf

... loenzyme. Keywords: phosphoprotein phosphatase; PP2A; subunit interactions; phosphorylation. Protein phosphatase 2A (PP2A) is an abundant cellular serine/threonine-speci®c phosphatase that regulates ... (2001) Protein phosphatase 2A: a highly regulated family of serine /threonin e phosphatase s implicated in cell growth and signalling. Biochem. J. 353, 417±439. 2. Virshup, D.M. (...

Ngày tải lên: 08/03/2014, 10:20

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Báo cáo Y học: The Saccharomyces cerevisiae type 2A protein phosphatase Pph22p is biochemically different from mammalian PP2A potx

... cerevisiae Pph22p phosphatase a unique enzyme among all type 2A protein phosphatases studied so far. Keywords: Saccharomyces cerevisiae; protein phosphatase Pph22p; protein phosphatase 2A; heterologous ... pastoris. Reversible protein phosphorylation catalysed by protein kinases and phosphoprotein phosphatases is a major mechanism utilized by eukaryotic organisms to regulate v...

Ngày tải lên: 08/03/2014, 22:20

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Báo cáo khoa học: MicroRNA-373, a new regulator of protein phosphatase 6, functions as an oncogene in hepatocellular carcinoma pdf

... the protein phosphatase 6 catalytic subunit (PPP6C ), a negative cell cycle regulator, was identiﬁed as a direct target gene of miR-373 by use of a ﬂuorescent reporter assay. The mRNA and protein ... 2044–2054 ª 2011 The Authors Journal compilation ª 2011 FEBS MicroRNA-373, a new regulator of protein phosphatase 6, functions as an oncogene in hepatocellular carcinoma Nannan Wu*, X...

Ngày tải lên: 14/03/2014, 23:20

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Báo cáo khoa học: Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identiﬁcation of kinase substrates doc

... ⁄ Thr protein kinase gene stkP linked to protein phosphatase phpP. Biochemical studies performed with recombinant StkP suggest that this protein is a functional eukaryotic-type Ser ⁄ Thr protein kinase. ... vitro and it is indeed a membrane protein as was predicted from amino acid sequence. PhpP is PP2C-type protein phosphatase To characterize a putative protein phosphatase...

Ngày tải lên: 16/03/2014, 18:20

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Báo cáo khoa học: Inhibitors of protein phosphatase 1 and 2A decrease the level of tubulin carboxypeptidase activity associated with microtubules pptx

... for phosphatase inhibitor to exert its inhibitory effect on the carboxypeptidase activity associated with microtubules. The experiments shown below address this point. Fig. 4. Eﬀect of protein phosphatase ... carboxypeptidase activity associated with microtubules is mediated by its capacity to inhibit protein phosphatases. Other phosphatase inhibitors (caly- culin A and cantharidin)...

Ngày tải lên: 23/03/2014, 15:21

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Báo cáo khoa học: Characterization of the role of a trimeric protein phosphatase complex in recovery from cisplatin-induced versus noncrosslinking DNA damage potx

... Pph, Saccharomyces cerevisiae protein serine ⁄ theonine phosphatase; Ppp ⁄ PP, protein serine ⁄ threonine phosphatase in mammals and Drosophila; Ppp4c, protein phosphatase 4 catalytic subunit ... initi- ated. Protein phosphorylation plays a key role in the DNA damage response, but the protein phosphatases Keywords cisplatin; histone 2AX; methylmethanesulfonate; PPH3; protein...

Ngày tải lên: 30/03/2014, 04:20

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