prins and in situ pcr protocols

... Mougin et al. 28. Embleton, M., Gorochov, G., Jones, P., and Winter, G. (1992) In- cell PCR from mRNA: amplifying and linking the rearranged immunoglobulin heavy and light chain V-genes within ... Molecular Biology, Vol 71: PRINS and In Situ PCR Protocols Edited by J. R Gosden Humana Press Inc , Totowa, NJ 23 24 Pellestor and Charlieu The primed in situ (P...

... the combined study, we will illustrate the in situ spatial organization of ammonia-oxidizing and nitrite-oxidizing bacteria on fine scale in autotrophic nitrifying biofilms by applying the full-cycle ... microsensors, and the spatial distributions of in situ nitrifying activities were determined. The relationship between the spatial organization of nitrifying bacterial popula...

... chromosomes in metaphase and interphase cells by in situ suppres- sion hybridization using recombinant DNA libraries. Hum. Genet. 80, 224–234. 3. Collins, C., Lin Kuo, W., Segraves, R., Fuscoe, J., Pinkel, ... the slides in wash solution C 3× for 3 min each. 13. Stain with DAPI by immersing the slides in 0.2 µg/mL of DAPI in 2× SSC for 5– 10 min at room temperature. Rinse in 2...

... transferring to TB buffer for 15 min. Rinse 3× for 4 min each time in PBS. 6. Block for 10 min in PBS + 2% BSA. Rinse 3× for 4 min each time in PBS. 7. Incubate with fluorochrome-linked streptavidin ... counterstain. Incubate cells 3 min in 0.2 µg/mL DAPI, and rinse once in PBS. 7. To label fixed samples using both TUNEL and immunofluorescence staining for an antigen of interest...

... DNA footprinting and in situ PCR it is necessary to label the PCR product directly. Direct labeling of PCR Analysis, sequencing and in vitro expression of PCR products 101 products also increases ... sequencing and in vitro expression of PCR products 89 Analysis, sequencing and in vitro expression of PCR products 5.1 Introduction Analysis of PCR products is cr...

... believed to act cooperatively in the cross-linking of proteins, including involucrin, loricrin and small proline-rich proteins (SPRs). Such concerted reactions result in formation of the cornified ... +3 (relative to the glutamine residue); (b) lysine or arginine mainly was found at position +2; and (c) in most sequences, an aromatic amino acid and a serine ⁄ threonine residue were...