mass spectrometry of proteins and peptides

... Biology TM HUMANA PRESS Mass Spectrometry of Proteins and Peptides Edited by John R. Chapman HUMANA PRESS Methods in Molecular Biology TM VOLUME 146 Mass Spectrometry of Proteins and Peptides Edited ... (1). Electrospray ionization mass spectrometry (ESI-MS) is a rapid and precise method for determining masses of proteins and peptides and can be used to...

Ngày tải lên: 11/04/2014, 09:50

... breakthrough in the field of proteo- mics and enables rapid sequencing of large peptides and intact proteins on a QLT mass spectrometer. The scan times required for direct analysis of proteins using ETD ... blocks of eukaryotic chromatin [15]. A host of PTMs reside on the N-terminal tails of the histones, and this array of PTMs has been well documented and include...

Ngày tải lên: 18/02/2014, 16:20

... Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation process of onconase 2 Marc Ribo ´ 1 , Montserrat Bosch 1 , ... solvent of the matrix, and 0.5 lL of the mixture was immediately applied to a sample probe spot. Cyclization reaction as a function of pH and temperature In order to determine the...

Ngày tải lên: 19/02/2014, 12:20

... Ala and Glu, Glu and Asp, and Glu and Gln is efficient in wheat germ extract but can effect- ively be suppressed by inhibitors of transaminases and glutamine synthase [20]. Among the multitude of ... synthesis of proteins in the presence of other pro- teins provided in excess at the start of or during the reaction, e.g., for the purpose of rescuing nascently produced in...

Ngày tải lên: 07/03/2014, 12:20

... classification of proteins identified in CRC Proteins identified by mass spectrometry were classified by subcellular location and molecular function (Fig. 3). To better understand the probable roles of the ... iTRAQ labeling mass spectrometry, a total of 438 proteins were identified by our proteomic platform. To better understand the roles of these identified proteins, they...

Ngày tải lên: 16/02/2014, 15:20

... combined use of electrospray mass spectrometry (ES-MS) and NMR spectroscopy. The branching pattern and blood group-related Lewis deter- minants, together with partial sequences and linkages of these ... different techniques, of which MS and NMR are two of the most powerful. Previously, we demonstrated the distinction of chain type and blood-group type (such as Le a/x...

Ngày tải lên: 19/02/2014, 12:20

... this mass range the density of the candidate pep- tide masses is relatively low. The levelling-off points out that the distribution of the alternative candidate peptide masses around the mass of ... and nomenclature of tandem mass spectra of chemically crosslinked peptides. J Am Soc Mass Spectrom 14, 834–850. 10 Clauser KR, Baker P & Burlingame AL (1999) Role of acc...

Ngày tải lên: 07/03/2014, 12:20

... resonance mass spec- trometry: a primer. Mass Spectrom Rev 17, 1–35. 56 Smith RD (2000) Evolution of ESI -mass spectrometry and Fourier transform ion cyclotron resonance for pro- teomics and other ... of MS in protein analysis because of its inherently ultra-high mass- resolving power and mass accuracy. For instance, protein–ligand [57], protein–carbohydrate [58,59], pro...

Ngày tải lên: 07/03/2014, 17:20

... fractions (M + 0) and (M + 1) of fragment 260 of Ala, fraction (M + 0) of fragment 288 of Val and all the measurements associated with fragment 186 of Val. All six measurements are redundant and can be ... atoms of aKG/Glu. The carbon atoms of AcCoA originate from the last two carbon atoms of pyruvate, the mass isotopomer distribution of which is reflected in this of...

Ngày tải lên: 08/03/2014, 08:20