... analysis of peptides and proteins N D Udeshi et al dimethylation of Arg, monomethylation, dimethylation and trimethylation of Lys, acetylation and ubiquitination of Lys, and phosphorylation of Ser and ... breakthrough in the field of proteomics and enables rapid sequencing of large peptides and intact proteins on a QLT mass spectrometer The scan times required for direct analysis of proteins using ETD ... than predicted Further analysis of c¢-type ions (singly and doubly ETD-MS analysis of peptides and proteins charged) indicates that the Tyr residues at positions 31 and 40 are both 80 mass units...
Ngày tải lên: 18/02/2014, 16:20
... rapid and precise method for determining masses of proteins and peptides and can be used to validate protein sequences (2,3) Mass accuracy is generally within 0.01% of the calculated mass for proteins ... (e.g., PicoFuge, Stratagene, Palo Alto, CA) Methods 3.1 In-Gel Digestion (see Notes and 9) 3.1.1 Excision of Protein Bands (spots) from Gels Rinse the entire gel with water and excise bands of interest ... dishes, and so on) may acquire a static charge and attract dust Accumulation of even a minute amount of dust in solutions and reagents results in massive contamination of samples with human and sheep...
Ngày tải lên: 11/04/2014, 09:50
Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
... the synthesis of proteins in the presence of other proteins provided in excess at the start of or during the reaction, e.g., for the purpose of rescuing nascently produced insoluble proteins into ... Ala and Glu, Glu and Asp, and Glu and Gln is efficient in wheat germ extract but can effectively be suppressed by inhibitors of transaminases and glutamine synthase [20] Among the multitude of ... need of sequence specific resonance assignments [10] One of the most attractive applications of combinatorial [15N]-labelling, however, may be for the identification of ligand binding sites on proteins...
Ngày tải lên: 07/03/2014, 12:20
báo cáo khoa học: "Self-assembly of proteins and their nucleic acids" docx
... fragment of the SAPV was amplified using ul of 16 pmol/ul M13F primer (part of the TOPO vector, upstream of the T7-F primer of the SAPV insert), 0.5 ul of 100 pmol/ul SA-11R primer and 0.5 ul of the ... 10 ul of each of the wash through and eluates from each assembly reaction were amplified in parallel using primers T7-F and T7TER-R 35 cycles of amplification of 1' at 96°C, 30" at 40°C and 1'30" ... molecule ligand or ligands (for example gluthatione), as a nucleic acid label, and an appropriate receptor or protein fragment which binds the ligand as part of the protein vector and which is...
Ngày tải lên: 11/08/2014, 00:21
analysis of proteins and protein complexes by chemical cross linking and mass spectrometry
... analyses of single protein and simple protein complex samples were mainly conducted on MALDI-TOF, MALDI-TOF/TOF, ESI-TOF (electrospray ionization-TOF) and ESI-Q-TOF (ESI-linear trap quadrupole-TOF) ... Enrichment of cross-linked peptides 20 1.3.1 Separation and digestion of cross-linked protein samples 20 1.3.2 Enrichment of cross-linked peptides 23 1.4 Analysis of cross-linked peptides by ... spectra of cross-linked peptides 70 3.4.3 The influence of different cross-linkers on the fragmentation of cross-linked peptides 74 3.4.4 The impact of resolution for MS2 spectra on interpretation and...
Ngày tải lên: 30/09/2015, 05:51
Intein mediated biotinylation of proteins and its application in protein microarray
... studies of proteins both in vitro and in vivo v List of Tables Table Page The influence of C-terminal residues on the in vivo cleavage of EGFP-intein and on-column cleavage/biotinylation of EGFP ... Biotinylation of three model proteins 24 3.2.1 Cloning of target genes into pTYB1 expression vector 24 3.2.2 Expression and extraction of fusion proteins 25 3.2.3 Affinity purification and on-column ... C-terminus of the yeast gene A standard PCR mixture contained 1x HotStarTaq DNA polymerase buffer (Qiagen), 0.2 mM of each dNTPs (NEB), 0.5 µM of each primer, 100 ng of plasmid DNA template and units of...
Ngày tải lên: 08/11/2015, 16:30
05 bacterial cultivation for production of proteins and other biological products (1)
... sparger, and numerous inlets and outlets for removal of samples and medium and for adding various solutions to the growing culture These include nutrients and growth factors to support growth and ... General layout of the bacterial cultivation process for production of proteins and other biological products 10.2.2 Instrumentation and Infrastructure Required To conduct a process of recombinant ... Volume DO and pH electrodes are installed; the bioreactor is filled with 45 liters of distilled water containing 250 g of yeast extract (Difco), 500 g of tryptone (Difco), 250 g of NaCl, 250 g of K2HPO4,...
Ngày tải lên: 24/04/2016, 10:44
Electrode formulation of si and lini0 5mn1 5o4 for li ion battery applied to electric traction
... presence of liquid electrolyte and the changes of volume which happens at the time of the insertion and the deinsertion of lithium in the AM [7] The choice of the non-electroactive additives and the ... 12wt% carbon black and 8wt% of CMC or PVdF or 4wt% of CMC and 4wt% of SBR Cells were charged and discharged at 150 mA/g between 0.17 and 0.9 V (b) Stress vs strain for CMC, CMCSBR, and PVDF films ... resource limitations excellent rate performance of Ni and Co High voltage, moderate Cost and resource limitations of Ni safety and Co Low cost and abundance of Mn, high voltage, Limited cycle life,...
Ngày tải lên: 19/05/2016, 10:44
Tài liệu Báo cáo khoa học: Angiopoietin-like proteins: emerging targets for treatment of obesity and related metabolic diseases pptx
... roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obesity and related metabolic diseases, and discuss whether these ANGPTLs could be targets for the prevention and treatment of these conditions Suppression of ... Induction Fig Schematic diagram showing the roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obesity and related metabolic diseases The expression of ANGPTL2 and ANGPTL6 ⁄ AGF is induced in obese conditions ... inflammation and systemic insulin resistance through the induction of vascular inflammation and monocyte migration ANGPTL6 ⁄ AGF antagonizes obesity and insulin resistance through the enhancement of systemic...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
... banding patterns and different glycoforms of PrPC In this study, we have analyzed the glycoform patterns of PrPC of human, sheep, cattle and mice and compared them Variable immunoreactivity of ... isoform (m) of PrPC were quantified and calculated as percentages of the total signal The glycoforms of the protein bands were analyzed as arithmetic means of separate gel runs The number of gel runs ... SAF34 and 8G8 for humans, SAF34, 8G8 and P4 for sheep, SAF34 and P4 for cattle, and SAF34 for mice; and for the C-terminal antibodies 6H4, SAF60 and SAF70 for humans, and mAbs 6H4, SAF60, SAF70 and...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... PGM1 and PGM4 targets) Effects of DNA cis-platination on sequencespecific DNA binding of p73 proteins We tested the influence of DNA modification with cisplatin on the binding of two p73 isoforms, ... with preparation of the p53 proteins, and Professor G Melino for donation of plasmids pcDNA3-HA-p73b and pcDNA3-HA-p73d This work was supported by IGA MH CR grant No NC ⁄ 7574-3 and partly by GACR ... respective supershifted bands are denoted as SR53, SR73d and SR73b; the presence of two supershifted bands in each of the lanes 5–7 corresponds to two possible stoichiometries of the antibody–protein...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx
... analysis of ligand binding to C-LytA (A) Conformational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands: s, d, 2.5 and 20 mM ... Inhibition of choline-binding proteins Fig Titration of the CD signal of C-LytA at 295 nm with ligands Symbols represent choline (d), atropine (n) and ipratropium (j) (A) Full range of ligand concentration ... Inhibition of choline-binding proteins B Maestro et al Thermal unfolding transition in the absence and in the presence of the different ligands The influence of ligand binding on the thermal stability of...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
... bonds between NH of Thr5 and CO of Arg2, as well as NH of Arg2 and CO of Thr5 Structure of cyclic hexapeptides – cYT peptide The NMR data of the cYT peptide were indicative of its flexible nature ... designed peptides are useful for inhibition of the T-cell adhesion mechanism Materials and methods Peptides The linear and cyclic peptides lER, lVY, cER, cVY, cEL and cYT (Table 1) were designed and ... activity of the peptides 12-mer linear and cyclic peptides The inhibitory activities of the peptides designed from rat CD2 were assayed by three methods In the first method, the inhibition of anti-CD58...
Ngày tải lên: 19/02/2014, 13:20
Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
... intraresidue contacts of Arg4) and between Thr3 and Pro5 the trNOE build-up of the b-protons of Asp2 was taken as reference For contacts between Arg4 and Pro5 the d-protons of Pro5 acted as reference ... bound and the free state For dimers and pentamers of the MUC-1 tandem repeat [16], i.e 40mer and 60mer peptides, the dissociation constant was determined to Kd ¼ 10)7 by SPR At an on-rate of kon ... resonances of Pro1 and Asp2 have the highest intensities in the STD spectrum, signals of Thr3 are of medium intensity, while the signals of Arg4 and Pro5 have the lowest intensity The d-protons of Pro5...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
... values) of Ni(II) and Cu(II) complexes of peptides at I = 0.1 M (KNO3) and 25 C Standard deviations on the last digits are given in parentheses EPR The X-band EPR spectra of Cu(II) complexes of VIHN ... Complexation of Cu(II) and Ni(II) by model peptides and albumins Among the systems under scrutiny in this work, the Ni(II) complexes of VIHN [11] and the DAHam complexes of Cu(II) and Ni(II) [31] ... Tables and thus present only the novel data: protonation constants for DAHKam and VIHN, and stability constants (log a values) of Cu(II)-VIHN, Cu-DAHKam and Ni-DAHKam systems The parameters of CD and...
Ngày tải lên: 22/02/2014, 04:20
NATIONAL REPORT OF MALAYSIA ON THE FORMULATION OF A TRANSBOUNDARY DIAGNOSTIC ANALYSIS AND PRELIMINARY FRAMEWORK OF A STRATEGIC ACTION PROGRAMME FOR THE BAY OF BENGAL potx
... existing information on the use of, and threats to, the Malaysian coastal and marine resources off the Straits of Malacca and the adjacent waters of the Andaman Sea and the Indian Ocean In the process, ... and sand, caused widespread degradation of areas with mineral deposits leaving behind sandy, barren and unfertile land, unattended water bodies, and silted waterways Landslips, particularly of ... central part of South-East Asia and occupies a total land area of 330,434 square kilometres The land mass comprises three main components: Peninsular Malaysia and the two states of Sabah and Sarawak,...
Ngày tải lên: 06/03/2014, 15:21
Báo cáo khoa học: Functional classification of scaffold proteins and related molecules pptx
... classification of scaffold proteins L Buday and P Tompa Table Scaffold proteins and their kin Representatives of the four categories of scaffold proteins and their relatives are presented The length of the ... classification of scaffold proteins L Buday and P Tompa the proteins enlisted represent variations on a common theme, binding signaling proteins together to direct and control the flow of information ... are often used interchangeably, which indicates that the functions of the proteins in the four categories overlap significantly and they are often difficult to distinguished This article, and the...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
... component of pre-mRNP particles, and is incorporated into pre-mRNAs by binding to a specific subset of RNA-binding proteins [4,22]; and (h) actin is a component of PICs and depletion of actin prevents ... polymerases and components of the transcription machinery in the nucleus The role of actin in transcription and its regulation Actin is both a major cytoskeletal component of all eukaryotic cells and ... that actin acts as a bridge between the polymerase and other constituents of the PIC [24]; and (d) actin and nuclear myosin (NM1), an isoform of myosin 1, are involved in transcription elongation...
Ngày tải lên: 07/03/2014, 01:20
Báo cáo khoa học: Protein transport in organelles: Dual targeting of proteins to mitochondria and chloroplasts ppt
... categorization of proteins dual targeted to mitochondria and chloroplasts in Arabidopsis The number of proteins in each classification is expressed as a percentage of the total number of proteins in ... dual targeting, and also what is required to avoid mis-sorting of proteins between mitochondria and chloroplasts The structures of binding of targeting peptides to mammalian and plant Tom20s ... outline of the future challenges in this field is provided, and the insights that may be gained from a greater understanding of the mechanism of dual targeting for the targeting of location-specific proteins...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Thermodynamic stability and folding of proteins from hyperthermophilic organisms doc
... Comparisons of the thermodynamics and kinetics of the folding of proteins from mesophilic and thermophilic ⁄ hyperthermophilic organisms can provide an insight into the mechanisms of stabilization ... Structural and genomic correlates of hyperthermostability J Biol Chem 275, 32383–32386 Thermodynamic stability and folding of proteins Das F & Gerstein M (2000) The stability of thermophilic proteins: ... (a) co-chaperonin proteins, (b) nonheme iron proteins, (c) DNA-binding proteins, as well as (d) a few other proteins Although the number of characterized hyperthermostable proteins is rather...
Ngày tải lên: 07/03/2014, 05:20