hplc of peptides and proteins, methods and protocols

... 20 METHODS IN MOLECULAR BIOLOGY TM Edited by Marie-Isabel Aguilar HPLC of Peptides and Proteins Volume 251 Methods and Protocols Edited by Marie-Isabel Aguilar Methods and Protocols HPLC of ... basis of the effect of ligand structure is not fully understood, a number of factors including the relative hydrophobicity and ligand chain length, flexibility,...

Ngày tải lên: 11/04/2014, 09:46

... Double Michael J. Thompson Telomeres and Telomerase HUMANA PRESS Methods in Molecular Biology TM VOLUME 191 Telomeres and Telomerase Methods and Protocols Methods and Protocols Edited by John A. Double Michael ... consists of tracts of simple, tandemly repeated sequences running 5' to 3' toward the distal ends of the chromo- some. In humans the sequence TAGGG...

Ngày tải lên: 11/04/2014, 07:12

... 5' end of the RNA; (2) the site of cleavage and polyadenylation, thus altering the 3' end of the transcript; and (3) the defi- nition of exons, providing for the different assortment of these ... standards of approximately the same size as the CTD fusion protein migrate two-thirds of the distance to the bottom of gel. Stain with Coomassie blue. Destain and moni...

Ngày tải lên: 11/04/2014, 09:53

... predetermined. Methods and Protocols Methods and Protocols Gene Therapy of Cancer M E T H O D S I N M O L E C U L A R M E D I C I N E TM Humana Press Edited by Wolfgang Walther Ulrike Stein Gene Therapy of ... Colombo and Rodolfo The events caused by the injection of cytokine-transduced tumors include both the early infiltration of granulocytes and then of macrophage...

Ngày tải lên: 11/04/2014, 09:42

... refrigerated microfuge for 15' and resuspend in 400 µL of 70% (v/v) ethanol. Centrifuge again and dissolve the pellet in 400 µL of TE buffer on ice. Add 50 µL of RNase solution and incubate ... Labeling of 5'-OH Ends of DNA 1. Prepare a reaction tube by adding 5 µL of 10× T4 kinase reaction buffer, 5 µL of sample from step 9 of Subheading 3.3.1., 5 µL (50 Units...

Ngày tải lên: 11/04/2014, 09:47

... Mucins Glycoprotein Methods and Protocols Isolation of Large Gel-Forming Mucins 11 Fig. 1. Density gradient centrifugation of cervical mucins and DNA. Purified cervical mu- cins were mixed with DNA and subjected ... monitor and collect fractions using a fraction col- lector and subject to the appropriate carbohydrate and antibody analyses. 3.6. Analysis of Mucins Method...

Ngày tải lên: 08/10/2012, 10:25

... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... 7.4, with 0.1% CaCl 2 at 37°C Histological Methods for Detection of Mucin 29 29 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The...

Ngày tải lên: 08/10/2012, 10:25

... 1 and 2 (2,3) of this volume for the preparation of secreted and membrane-associated mucins, respectively, and to Chapter 7 (4) for a discussion of methods for mucin separation. 54 McGuckin and ... Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi- cation of mucins and their glycoforms, in Glycoprotein Methods and Protocols: T...

Ngày tải lên: 08/10/2012, 10:25

... secretion of mucin of the gut, the adherent mucous gel is frequently ignored because of the practical difficulties in observing and quantitating it. Quantitative measurement of the amount of mucin ... the hydration of exposed mucosa and the degree of stirring of the solution above the mucosa, respectively. Consequently, the dimensions of mucous thickness measured by the...

Ngày tải lên: 08/10/2012, 10:25

... Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... Totowa, NJ 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. Introduction Most ... au/milliliter...

Ngày tải lên: 08/10/2012, 10:25