dna replication

... hydrolysis of DnaA AT-rich region DnaA box DnaB DnaC Fig. 1. Cartoon of the assembly of the DNA replication machinery on the E. coli oriC region. Binding of DnaA (green ovals) to the DnaA boxes ... of DnaC severely inhibits the heli- case activity of DnaB and also increases the affinity of DnaC for single stranded DNA. In contrast ADP– DnaC does not inhibit DnaB helicase and has lower D...

Ngày tải lên: 07/03/2014, 21:20

... Several techniques used for analysis of DNA replication in C. elegans are summarized in BOX 1. 3. The factors that regulate DNA replication The regulation of DNA replication in eukaryotes involves ... Clinical Application 5 3. DNA replication and MCM2-7 family proteins Prior to DNA replication and during late M and G1 phases of the cell cycle, MCM2-7 form the pre -...

Ngày tải lên: 08/03/2014, 19:20

... required for DNA replication through heterochromatin. Nat. Genet. 32, 627–632. 52. Lipford, J.R. & Bell, S.P. (2001) Nucleosomes positioned by ORC facilitate the initiation of DNA replication. ... ARTICLE Chromatin dynamics at DNA replication, transcription and repair Ann E. Ehrenhofer-Murray Otto-Warburg-Laboratories, Max-Planck-Institute of Molecular Genetics, Berlin, Germany...

Ngày tải lên: 16/03/2014, 16:20

... T antigens with the DNA replication initiation complex. Keywords: DNA replication; initiation; DNA polymerase a-primase; species specificity; polyomavirus. Polyomavirus DNA replication has served ... JCV DNA replication on human replication proteins. Figure 1 repre- sents a comparison between the SV40 (panel A) and JCV (panel B) cell-free DNA replication systems. Figure...

Ngày tải lên: 17/03/2014, 03:20

... initiation of DNA synthesis. While all cells encode multiple different DNA polymerases the enzymes with the most well characterized roles in nuclear chromosomal DNA replication are DNA Polε, DNA Polδ, ... bidirectional dsDNA unwinding and replication fork movement along the chromosome allowing the synthesis of new DNA. Initiating DNA replication is a serious event for a ce...

Ngày tải lên: 30/03/2014, 00:20

... of a DNA repli- cation fork revealed by reconstitution of SV40 DNA replication in vitro. Nature 369, 207–212. 7 Tsurimoto T & Stillman B (1991) Replication factors required for SV40 DNA replication ... pro- gression and DNA replication, rather than a DNA repair or damage response function. Furthermore, the polymerase trap and inhibition of replication in isolated nuclei...

Ngày tải lên: 30/03/2014, 10:20

... of these processes to DNA replication. Methods of analyzing DNA replication in vivo are also included. JUDITH L. CAMPBELL xiii 16 DNA POLYMERASES [2] stranded DNA from n to n-1 nucleotides ... measuring hydrolysis of single-stranded DNA: Single-stranded DNA, ~ DNA, , ~ + dNMP. Pol II (0.1 to 1 unit) is added to 40 txl 5'-32p-labeled single-stranded DNA reactio...

Ngày tải lên: 11/04/2014, 09:40

... used to hybridize to certain sequences of DNA • recombinant DNA can be joined to existing DNA using DNA ligase Cloning DNA using bacteria • foreign DNA can be introduced into bacterial cells ... Initiator proteins bind at replication origins and recruit DNA replication machinery proteins • DNA polymerase is responsible for catalyzing synthesis of new strands Replicat...

Ngày tải lên: 13/03/2014, 19:38

... biochemical properties, such as low fidelity on undamaged DNA [6] Keywords Arabidopsis thaliana; base-pair mismatch; DNA damage; DNA replication; translesion DNA synthesis Correspondence R. R. Ariza, Departamento ... spe- cialized DNA polymerases that play essential functions in repair and ⁄ or replication of damaged DNA [1–3]. Many of these enzymes are structurally related and...

Ngày tải lên: 16/03/2014, 10:20

... gel agarose 1 % Đề cương thực tập tốt nghiệp Đề tài:    “  Nghiên cứu ứng dụng chỉ thị phân tử  DNA để chọn lọc gen tms2 tạo ra các dòng  TGMS mới” Người hướng dẫn : 1.PGS.TS.Phan Hữu Tôn                                   2.KS.Tống Văn Hải                     Bộ môn : Công nghệ sinh học ứng dụng                     Khoa CNSH­Trường ĐHNN­Hà Nội Người thực hiện : SV.Nguyễn Thị Hồng Hạnh Lớp   ...

Ngày tải lên: 19/08/2012, 00:04