psychiatric genetics, methods and reviews

... surpris- 3 From: Methods in Molecular Medicine, vol. 77: Psychiatric Genetics: Methods and Reviews Edited by: M. Leboyer and F. Bellivier © Humana Press Inc., Totowa, NJ Psychiatric Genetics: Analytical Methods ... E TM Psychiatric Genetics Methods and Reviews Edited by Marion Leboyer, MD, PhD Frank Bellivier, MD, PhD Psychiatric Genetics: Analytical Methods 23 23...

Ngày tải lên: 11/04/2014, 07:10

... drugs Suicide Gene Therapy Methods and Reviews Edited by Caroline J. Springer M E T H O D S I N M O L E C U L A R M E D I C I N E TM Suicide Gene Therapy Methods and Reviews Edited by Caroline ... (at 1 mM 5-FC) and 92% (at 2 mM 5-FC) of the cell population. 14 Niculescu-Duvaz and Springer 14 Table 2 (continued) Bystander Effect No. GDEPT system Bystander effect in vitro Bysta...

Ngày tải lên: 11/04/2014, 10:33

... UV monitor and collect fractions using a fraction col- lector and subject to the appropriate carbohydrate and antibody analyses. 3.6. Analysis of Mucins Methods for the detection and analysis ... antibodies and lectins or coating methods such as the glycan detection method and enzyme-linked immunosorbent assays (ELISA). All these techniques have advantages and disadvan- tage...

Ngày tải lên: 08/10/2012, 10:25

... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... such as Culling’s Handbook of Histopathological and Histochemical Techniques (including museum techniques) (36) or Kiernan’s Histological and Histochemical Methods: Theory and...

Ngày tải lên: 08/10/2012, 10:25

... Inc., Totowa, NJ 4 Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods Michael A. McGuckin and David J. Thornton 1. Introduction Detection and quantitation of mucins ... 1 and 2 (2,3) of this volume for the preparation of secreted and membrane-associated mucins, respectively, and to Chapter 7 (4) for a discussion of methods for mucin separation....

Ngày tải lên: 08/10/2012, 10:25

... poly- L -lysine for 5 min and dried at room temperature. 3. Methods 3.1. Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections ( see Notes 1–4) This is a rapid and simple method ... will be greatly reduced and discontinuous. 7. Careful handling of the cryostat sections on the slide during fixing and staining is impor- tant because excessive washing and so...

Ngày tải lên: 08/10/2012, 10:25

... Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield ... NJ 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. Introduction Most epithelial ... PhosphorImager. 4...

Ngày tải lên: 08/10/2012, 10:25

... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... Separation and Identification of Mucin 77 7 Separation and Identification of Mucins and Their Glycoforms David J. Thornton, Nagma Khan, and John K. Sheehan 1. Introduction This ......

Ngày tải lên: 08/10/2012, 10:25

... Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: ... chapter describes only methods for assessing the heterogeneity of mucin populations with regard to mass and size utilizing rate zonal centrifugation and electron microscopy. Other methods for the absolute ....

Ngày tải lên: 08/10/2012, 10:25