differentiation of embryonic stem cells

... are often larger, less red and contain smaller cells. Cells in the definitive erythroid colonies express bmajor but no bH1 globin. Pure megakaryocyte colonies consist of relatively few (10–50) cells. ... of relatively few (10–50) cells. Most of these colonies contain cells of quite different sizes, representing different stages of megakaryocyte development. These cells tend to...

Ngày tải lên: 11/04/2014, 00:32

... to ES cells (77) is greater than that of EG cells to ES cells (49), confirming the similarity of EG cells to ES cells. Epigenetic study of embryonic stem cells N. Hattori and K. Shiota 1626 FEBS ... It ICM TE PGC TS cells ES cells EG cells 4977 Placental cells Embr y onic cells Fig. 1. Epigenetic distances between ES cells and other stem cells derived from...

Ngày tải lên: 23/03/2014, 07:20

... 755 Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells Padraic ... erythroid differentiation of murine ES cells in vitro. In these experiments we utilized the ES ⁄ OP9 cell in vitro differentiation system described by Kitayima et al. [20]....

Ngày tải lên: 07/03/2014, 12:20

... and power failure. Key words: human embryonic, stem cells, low temperature 1. Introduction In vitro culture of human embryonic stem cells (hESC) often involves temporary exposure to reduced ... Published: 2006.07.25 This study investigated the effects of exposing human embryonic stem cells (hESC) to 4 o C and 25 o C for extended durations of 24h and 48h respectivel...

Ngày tải lên: 31/10/2012, 16:57

... involved in up-regulation of human deoxyribonuclease II transcription during differentiation of HL-60 cells San-Fang Chou 1 , Hui-Ling Chen 2 * and Shao-Chun Lu 1 * 1 Department of Biochemistry and ... in apoptotic cells after they are engulfed by phagocytic cells. Mice with targeted disruption of the DNase II gene die at birth because of severe anemia [10] and/or asphyxiati...

Ngày tải lên: 23/03/2014, 17:21

... DBA/1LacJ ES cells (12) meet or exceed that criterion Murine Embryonic Stem Cells 1 at least 86% of the time, whereas our 129 strain of ES cells meet or exceed the criteria 45% of the time. The ... Biology, vol. 185: Embryonic Stem Cells: Methods and Protocols Edited by: K. Turksen © Humana Press Inc., Totowa, NJ 1 Methods for the Isolation and Maintenance of Murine...

Ngày tải lên: 08/04/2014, 12:51

... specific features of the plasticity of adult stem cells are largely unknown. Recently, we demonstrated the hepatic differentiation of human adipose tissue-derived mesenchymal stem cells (AT-MSCs). ... GM, Claas FH, Fibbe WE & Kanhai HH (2004) Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta. Stem Cells 22, 1338–1345. 6 Bieback...

Ngày tải lên: 30/03/2014, 04:20

... growth of most kinds of cells, promote the proliferation of some types of cells. There is increasing evidence that mild hypoxia acts as a potent regulator of various types of stem cells [12]. Therefore, ... O 2 ) for 3 days. The percentage of GFP-positive cells was about 70% of that of total neural stem cells after 3 days of transfec- tion (Fig. 4A). The expres...

Ngày tải lên: 30/03/2014, 04:20

... Treatment of Femoral Head Necrosis with Arterial Perfusion of Marrow Stem Cells. Chin J Integr Trad West Med. 2004,24(11):999-1002 5. Yan Z, Hang D, Guo C, Chen Z. Fate of mesenchymal stem cells ... improvement of blood circulation in the necrotic femoral head and sur- rounding tissues [4]. Mesenchymal stem cells (MSCs) are multipotent stem cells that can differenti...

Ngày tải lên: 25/10/2012, 11:18

... T, Kitamura S & Nagaya N (2007) Effect of hypoxia on gene expression of bone marrow- derived mesenchymal stem cells and mononuclear cells. Stem Cells 25, 1166–1177. 7 Li L, Zhang S, Zhang ... (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284, 143–147. 2 Porada CD, Zanjani ED & Almeida-Porad G (2006) Adult mesenchymal stem cells: a pl...

Ngày tải lên: 18/02/2014, 04:20