dendritic cell protocols

... D S I N M O L E C U L A R M E D I C I N E TM Dendritic Cell Protocols Edited by Stephen P. Robinson, MD, PhD Andrew J. Stagg, PhD Dendritic Cell Protocols Edited by Stephen P. Robinson, MD, PhD Andrew ... M. D., and Steinman, R. M. (1989) The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from dif- ferent tissues including thymus. Cell. Immunol. 118...

Ngày tải lên: 11/04/2014, 00:19

... and direction changes of individual cells (together referred to as “tracking” assays) as well as determinations of cell orientation, cell cell inter- actions, cell morphology, flagellar wave patterns, ... by Heide Schatten Germ Cell Protocols Volume 1: Sperm and Oocyte Analysis Volume 253 METHODS IN MOLECULAR BIOLOGY TM METHODS IN MOLECULAR BIOLOGY TM Edited by Heide Schatten Germ...

Ngày tải lên: 11/04/2014, 09:43

... donor cells, either ES, EC, or germ cells could be used. In this study, germ cells are used as they match cumulus cells for direct recovery from the body and absence of in vitro culture. Gonadal cells, ... reactivation (cumulus cell) and maintenance (germ cell) of transgene expression and, hence, for pluripotency. The reconstructed oocytes were scored for GFP at the two -cell, four -cell, e...

Ngày tải lên: 11/04/2014, 09:43

... Cell Cycle Analysis of Stem Cells 101 3.2. Staining of Partially Purified Populations of Cells with Hst, Pyronin Y, and a Cell- Surface Marker Partially purified cell populations ... Thy-1 lo Sca-1 + Lineage - Mac-1 + CD4 – cells represent approx 0.04% of fetal liver cells from E12.5 to E14.5, but only approx 0.015% of cells at E15.5 (23). At least 25% of these cells are in S/G 2 /M pha...

Ngày tải lên: 11/04/2014, 09:45

... target cells from lysis by Ly49-deficient fetal NK cells. Eur. J. Immunol. 28, 47–56. Fetal Mouse NK Cells 13 13 From: Methods in Molecular Biology, vol. 121: Natural Killer Cell Protocols: Cellular ... Biology VOLUME 121 HUMANA PRESS HUMANA PRESS Natural Killer Cell Protocols Cellular and Molecular Methods Natural Killer Cell Protocols Cellular and Molecular Methods TM TM Edited...

Ngày tải lên: 11/04/2014, 09:58

... step is to remove most cells bearing mark- ers of mature thymocytes, of more mature precursor cells and other non-T lin- eage cells. Add to the cell pellet 10 µL/10 6 cells of the depletion MAb ... total thymocytes. It is not exclusively T-lineage committed and retains the potential to form NK cells, B cells and dendritic cells (DC) (4–6). The low CD4 precur- sor population then loses sur...

Ngày tải lên: 11/04/2014, 10:34

... primary cells because of the increased risk of bacterial contamination associated with the isolation procedures. For primary cells and newly acquired cell lines, it is advisable to culture cells with ... density of approx 1 × 10 5 cells/mL works well for P815 cells. To ensure continued exponential growth, the cell density should be maintained between 1 × 10 5 and 1 × 10 6 cells/mL. Ref...

Ngày tải lên: 08/04/2014, 12:50