2the skin dendritic cell network

... production of dendritic cells from blood or bone marrow) Langerhans cells served as a prototype dendritic cell The basis of our current knowledge on the maturation of dendritic cells stems from ... subclasses of dendritic cells direct the development of distinct T helper cells in vivo J Exp Med 189, 587–592 Suss, G and Shortman, K (1996) A subclass of dendritic cells kills CD4 T cells via Fas/Fas-ligand- ... superantigens by thymic and splenic dendritic cells and B cells J Immunol 157, 2789–2794 Ardavin,C., Wu, L., Li, C.-L., and Shortman, K (1993) Thymic dendritic cells and T cells develop simultaneously...

Ngày tải lên: 11/04/2014, 00:19

... Rho GTPase activity in dendritic cells J Immunother 2008, 31(5):491-499 Shurin GV, Amina N, Shurin MR: Cancer therapy and dendritic cell immunomodulation In Dendritic Cells in Cancer Edited by: ... Shurin GV: Mechanisms of dendritic cell- induced T cell proliferation in the primary MLR assay Immunol Lett 2001, 78(2):75-82 Steinman RM, Witmer MD: Lymphoid dendritic cells are potent stimulators ... Optimizing dendritic cell- based immunotherapy for cancer Expert review of vaccines 2007, 6(3):333-345 Chao D, Bahl P, Houlbrook S, Hoy L, Harris A, Austyn JM: Human cultured dendritic cells show...

Ngày tải lên: 18/06/2014, 15:20

... noncoding B cell integration cluster (Bic) gene [23] and is functionally important in B cell, T cell and macrophage biology miR-155 is up-regulated in B cells exposed to antigen, in T cells stimulated ... generation of dendritic cells J Immunol Methods 2005, 298:61-72 11 Albert ML, Jegathesan M, Darnell RB: Dendritic cell maturation is required for the cross-tolerization of CD8+ T cells Nat Immunol ... expressed in human activated and mature dendritic cells through serial analysis of gene expression Blood 2000, 96:2206-2214 Young JW, Merad M, Hart DN: Dendritic cells in transplantation and immune-based...

Ngày tải lên: 18/06/2014, 16:20

... NC, USA) B16 mouse melanoma cell line and EL4 mouse lymphoma cell line were obtained from Shanghai Institute of Cell Biology and Biochemistry (Shanghai, China) All of cell lines were maintained ... Contassot E, Beermann F, Huard B, French LE: Tumor-infiltrating dendritic cells are potent antigen-presenting cells able to activate T cells and mediate tumor rejection J Immunol 2006, 176:61-67 ... an effective antitumor immune response is critically dependent on dendritic cells (DCs), the most effective antigen-presenting cells (APCs) that capture antigens in peripheral tumor tissues and...

Ngày tải lên: 18/06/2014, 16:20

... incubated in tissue culture flasks with CellGro DC medium (Cell Genix, Freiburg, Germany) at a density of 107 cells/ ml for hr, and the adherent cells were incubated in CellGro DC medium containing 1000 ... developing dendritic cells Science 2000, 288:522-527 10 Cella M, Engering A, Pinet V, Pieters J, Lanzavecchia A: Inflammatory stimuli induce accumulation of MHC class II complexes on dendritic cells ... Adams S, Bhardwaj N: Manipulating dendritic cell biology for the active immunotherapy of cancer Blood 2004, 104:2235-2246 Banchereau J, Palucka AK: Dendritic cells as therapeutic vaccines against...

Ngày tải lên: 18/06/2014, 16:20

... induced in vitro by CD16- and CD16+ monocytederived dendritic cells in patients with metastatic renal cell carcinoma treated with dendritic cell vaccines J Clin Immunol 2004, 24:86-96 Aricò et ... transmenbrane G-protein-coupled receptor CXCR3, and is expressed especially in activated Th1 cells, B cells, NK cells and DC, thus suggesting that CXCL10 release can represent a primary event in the ... (Wilcoxon Matched Pairs test) List of Abbreviations IFN: Interferon; DC: Dendritic Cells; PBMC: Peripheral Blood Mononuclear Cells; Th: T helper; CTL: Cytotoxic T Lymphocytes; HCV: Hepatitis C Virus;...

Ngày tải lên: 18/06/2014, 19:20

... follicular dendritic cells regulates the B cell recall response J Immunol 2000;164: 6268–75 30 Sozzani S, Longoni D, Bonecchi R, et al Human monocyte-derived and CD34+ cell- derived dendritic cells ... centre cells, follicular dendritic cells and germinal centre T cells produce B cell- stimulating cytokines Cytokine 1995;7:344–54 34 Szakal AK, Kapasi ZF, Haley ST, Tew JG A theory of follicular dendritic ... et al Follicular dendritic cells, interdigitating cells, and cells of the monocyte-macrophage lineage are the C1q-producing sources in the spleen Identification of specific cell types by in situ...

Ngày tải lên: 08/08/2014, 20:23

... 40-micron cell strainer to prepare single -cell suspensions This is a standard method of cell preparation and does not result in selective loss or enrichment of lymphocytes or dendritic cells Viabilities ... through a cell strainer to produce single -cell suspensions The cells were pelleted by centrifugation at 500g for minutes at 4uC, and then red blood cells were lysed by resuspending the cell pellets ... nodes through 40-micron cell strainers, and CD11c+ cells were isolated by magnetic bead separation The cells were stained for CD11b and separately for each of the dendritic cell activation markers...

Ngày tải lên: 08/08/2014, 21:20

... Steinbrink K, Enk AH: Dendritic cells as a tool to induce anergic and regulatory T cells Trends Immunol 2001, 22:394-400 Thompson AG, Thomas R: Induction of immune tolerance by dendritic cells: implications ... Results Assessment of T -cell stimulatory capability Stimulator cells were irradiated (3000 rad, 137Cs source) and added at increasing cell numbers to × 105 allogeneic T cells in 96-well culture ... consequence of cellular cytotoxicity, as indicated by unchanged cell morphology and viability (percentage PI positivity was 9.0 ± 2.9% in untreated cells versus 15.3 ± 0.5% in cells treated with...

Ngày tải lên: 09/08/2014, 06:22

... K: Immunobiology of dendritic cells Annu Rev Immunol 2000, 18:767-811 10 Liu YJ: Dendritic cell subsets and lineages, and their functions in innate and adaptive immunity Cell 2001, 106:259-262 ... Hartmann G: Plasmacytoid dendritic cells, antigen, and CpG-C license human B cells for plasma cell differentiation and immunoglobulin production in the absence of T -cell help Blood 2004, 103:3058-3064 ... C: Critical role of IL-12 in dendritic cell- 18 20 induced differentiation of naive B lymphocytes J Immunol 1998, 161:2223-2231 MacLennan I, Vinuesa C: Dendritic cells, BAFF, and APRIL: innate...

Ngày tải lên: 09/08/2014, 06:23

... Jongbloed et al Figure Enumeration of dendritic cell subsets in various arthritides and healthy volunteers Plasmacytoid dendritic cells (pDCs) and myeloid dendritic cells volunteers (mDCs) are significantly ... number of cells (×104/ml) in rheumatoid arthritis and psoriatic arthritis peripheral blood and matched synovial fluid White blood cells Myeloid dendritic cells Plasmacytoid dendritic cells Rheumatoid ... mature antigen-presenting dendritic cells J Immunol 1994, 152:2613-2623 24 Highton J, Kean A, Hessian PA, Thomson J, Rietveld J, Hart DN: Cells expressing dendritic cell markers are present in...

Ngày tải lên: 09/08/2014, 07:20

... Germany), using DAPI for nuclear staining Cell culture and generation of IFIT4-primed dendritic cell- like cells from THP-1 cells The human monocytic cell line THP-1 (ATCC, Manassas, VA, USA) ... into dendritic cells (DCs) [19-26], antigen-presenting cells that induce and regulate immune responses An increased number of circulating plasma cells and the presence of autoreactive T and B cells ... immune cell, IFIT4 was expressed in many immune cells, including CD19+, CD8+, CD14+ and CD4+ cells (P < 0.05; Figure 3b) To determine the the subcellular localization of IFIT4 protein, THP-1 cells...

Ngày tải lên: 09/08/2014, 10:23

... complex-stimulated AM14 B cells and dendritic cells B cells were isolated from AM14 BCR transgenic mice by positive selection using anti-B220-coupled magnetic beads [17] AM14 B cells were stimulated ... non-autoreactive B cells also applies to AM14 B cells INH-ODNs made with phosphorothioate backbone inhibit activation of dendritic cells, macrophages, and RNA/anti-RNA-stimulated AM14 B cells in a sequenceindependent ... Hartmann G: Plasmacytoid dendritic cells, antigen, and CpG-C license human B cells for plasma cell differentiation and immunoglobulin production in the absence of T -cell help Blood 2004, 103:3058-3064...

Ngày tải lên: 09/08/2014, 14:21

... induce CD4+ T helper cell immune responses CD4 + T helper cells are known to play an important role in the generation of CD8+ T cell immune responses as well as memory T cell responses (for review ... harvested and stimulated with the E7 or PADRE peptide Cells were characterized for E7-specific CD8+ T cells or PADRE-specific CD4+ T cells using intracellular IFN-g staining followed by flow cytometry ... harvested and stimulated with the E7 or PADRE peptide Cells were characterized for E7-specific CD8+ T cells or PADRE-specific CD4+ T cells using intracellular IFN-g staining followed by flow cytometry...

Ngày tải lên: 10/08/2014, 05:21

... CD8+ T -cell proliferation After indicated treatments, treated DCs were co-cultured with CD4+ T cells or CD8+ T cells in a ratio of 1:20 (DCs versus CD4+ T cells or CD8+ T cells) for 72 h T -cell ... agents on expression of cell- surface markers and CD4+ and CD8+ T -cell proliferation in mouse bone marrow-derived immature dendritic cells (A) Expression of surface markers on DCs Cells were treated ... http://www.jbiomedsci.com/content/18/1/44 Dendritic cells pulsed with tumor cell lysates DCs were suspended in RPMI-1640 medium at a concentration of × 106 cells/3 ml in 6-well plate Tumor cell lsysates (TCLs, 400...

Ngày tải lên: 10/08/2014, 05:21

... certification of absence of contamination Generation of dendritic cells After informed consent, the mature dendritic cells of autologous mononuclear cells were isolated by the FicollHypaque density ... CD8), NK cells (CD56), B lymphocytes (CD19) and mature dendritic cells (CD86, CD80, CD83, CD40 and HLA-DR) were used for immunophenotyping of the patients’ blood cells Approximately × 105cells per ... antigen-presenting cells, like dendritic cells, play an important role in the induction of an immune response, and an imbalance in the proportion of macrophages, immature and mature dendritic cells within...

Ngày tải lên: 10/08/2014, 10:21

... another three cell connection experiment That is, two cardiomyocyte cells were connected by a HeLa cell HeLa cells are one of the cell line and is not regarded as the connecting cells between ... also should compare the heterogeneous cell networks against the homogeneous cell networks Hence we have examined the synchronization of the two-cardiomyocyte network having a fibroblast connection, ... higher complexity of cellular system, functional cell- network, we have examined the meaning and contribution of fibroblasts in the cardiomyocyte network using on-chip single -cell- based cultivation...

Ngày tải lên: 11/08/2014, 00:23

... Figdor CG, Punt CJ, Adema GJ: Immunomonitoring tumor-specific T cells in delayed-type hypersensitivity skin biopsies after dendritic cell vaccination correlates with clinical outcome J Clin Oncol ... CG, et al: Vaccination of colorectal cancer patients with CEA-loaded dendritic cells: antigen-specific T cell responses in DTH skin tests Ann Oncol 2006, 17:974-980 Bonehill A, Van Nuffel AM, Corthals ... Th17 cells in the human tumor environments Blood 2009, 114:1141-1149 Boczkowski D, Lee J, Pruitt S, Nair S: Dendritic cells engineered to secrete anti-GITR antibodies are effective adjuvants to dendritic...

Ngày tải lên: 11/08/2014, 08:21

... cell response to column-activated malignant T cell loaded DC CD8 T cell response to column-activated malignant T cell loaded DC Fig 10A: CD8 T cells were magnetic-bead enriched (≥96% CD8+ T cells) ... patient The cells were co-cultured overnight with 1.1 × 106 CD8 T cells/well added to CD3-bead rendered apoptotic CTCL cells or viable CTCL cells (4 × 106/well) After overnight culture, the cells were ... lymphocyte population Percent apoptotic cells found in co-cultures of a: CD8 T cells and DC loaded with apoptotic malignant T cells; b: CD8 T cells, DC and viable CTCL cells; c: DC loaded with apoptotic...

Ngày tải lên: 11/08/2014, 10:23