asthma, mechanisms and protocols
asthma, mechanisms and protocols
... history, and rela-
tionship of wheezy bronchitis and asthma in children. An epidemiological study.
Br. Med. J. 4, 321–325.
22. Strachan, D. P., Butland, B. K., and Anderson, H. R. (1996) Incidence and ... bronchitis, and in emphysema. Thorax 24, 176–179.
48. Takizawa, T. and Thurlbeck, W. M. (1971) Muscle and mucous gland size in the
major bronchi of patients with chronic bronch...
cell cycle control mechanisms and protocols methods in molecular biology - tim humphrey, gavin brooks
... Cycle
Control
Volume 296
Mechanisms and Protocols
Edited by
Tim Humphrey
Gavin Brooks
Cell Cycle
Control
Mechanisms and Protocols
The Budding and Fission Yeasts 21
22. Saiz, J. E. and Fisher, R. P. ... NJ
1
Cell Cycle Molecules and Mechanisms of the Budding
and Fission Yeasts
Tim Humphrey and Amanda Pearce
Summary
The cell cycles of the budding yeast Saccharomyces cere...
Glycoprotein Methods and Protocols - P1
... PRESS
Glycoprotein
Methods
and Protocols
Edited by
Anthony P. Corfield
VOLUME 125
The Mucins
Methods in Molecular Biology
TM
HUMANA PRESS
Edited by
Anthony P. Corfield
The Mucins
Glycoprotein
Methods
and Protocols
Isolation ... UV monitor and collect fractions using a fraction col-
lector and subject to the appropriate carbohydrate and antibody analyses.
3.6. Analysis of Mucins...
Glycoprotein Methods and Protocols - P2
... nature and
limitations of the individual techniques, and (2) interpretation and reporting of mucin
staining.
1.1. Nature and Limitations of Mucin-Staining Methods
Mucin histochemistry, lectin, and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa,
NJ.
50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties, Func...
Glycoprotein Methods and Protocols - P3
... Methods and
Protocols: The Mucins (Corfield, T. ed.), Humana, Totowa, NJ.
4. Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi-
cation of mucins and their ... sample volume, and superior wash-
ing). For quantitation and comparison across blots, a standard in the same buffer as
samples should be titrated for use as a standard curve, and samp...
Glycoprotein Methods and Protocols - P4
... will be greatly
reduced and discontinuous.
7. Careful handling of the cryostat sections on the slide during fixing and staining is impor-
tant because excessive washing and so on can remove mucus ... to rat, frog and human gastric mucosa: influence of feeding
prostaglandin, N-acetyl-cysteine and other agents. Clin. Sci. 63, 187–195.
4. Bell, A. E., Allen, A., Morris, E. R., and Ro...
Glycoprotein Methods and Protocols - P5
... NJ
6
Quantitation of Biosynthesis
and Secretion of Mucin Using Metabolic Labeling
Jan Dekker, B. Jan-Willem Van Klinken,
Hans A. Büller, and Alexandra W. C. Einerhand
1. Introduction
Most epithelial ... PhosphorImager.
4. The identification of mucin precursors and mature mucins using polypeptide-specific
antisera and SDS-PAGE is elaborated in Chapters 20 and 21, and in several ref...
Glycoprotein Methods and Protocols - P6
... small gel, use 1.6 g of
agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of
buffer.
2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... Separation and Identification of Mucin 77
7
Separation and Identification
of Mucins and Their Glycoforms
David J. Thornton, Nagma Khan, and John K. Sheehan
1. Introduction
This ......
Glycoprotein Methods and Protocols - P7
... K. E., and Chapman J. A. (1985) Mica sandwhich
technique for preparing macromolecules for rotary shadowing. J. Ultrastruct. Res. 91,
66–76.
14. Kawahara K and Tanford C. (1966) Viscosity and density ... MUC5B, and MUC6 (1–4)—are gel-forming mucins, and it is possible
that there are others. In isolating a mixture of mucins from mucus, there is consider-
able scope for heterogeneity i...
Glycoprotein Methods and Protocols - P8
... caramelized and further charcoaled, and the acid hydrolysis results in a
black residue. This residue appears to precipitate protein/amino acids, interferes with
114 Yan and Packer
chromatography, and ... 2 min for pump and column equilibration with the initial composition of
the mobile phase.
5. Figures 1 and 2 show typical chromatograms of the separation of amino acid standards...