asthma, mechanisms and protocols
asthma, mechanisms and protocols
... history, and rela- tionship of wheezy bronchitis and asthma in children. An epidemiological study. Br. Med. J. 4, 321–325. 22. Strachan, D. P., Butland, B. K., and Anderson, H. R. (1996) Incidence and ... bronchitis, and in emphysema. Thorax 24, 176–179. 48. Takizawa, T. and Thurlbeck, W. M. (1971) Muscle and mucous gland size in the major bronchi of patients with chronic bronch...
Ngày tải lên: 10/04/2014, 22:28
cell cycle control mechanisms and protocols methods in molecular biology - tim humphrey, gavin brooks
... Cycle Control Volume 296 Mechanisms and Protocols Edited by Tim Humphrey Gavin Brooks Cell Cycle Control Mechanisms and Protocols The Budding and Fission Yeasts 21 22. Saiz, J. E. and Fisher, R. P. ... NJ 1 Cell Cycle Molecules and Mechanisms of the Budding and Fission Yeasts Tim Humphrey and Amanda Pearce Summary The cell cycles of the budding yeast Saccharomyces cere...
Ngày tải lên: 08/04/2014, 12:50
Glycoprotein Methods and Protocols - P1
... PRESS Glycoprotein Methods and Protocols Edited by Anthony P. Corfield VOLUME 125 The Mucins Methods in Molecular Biology TM HUMANA PRESS Edited by Anthony P. Corfield The Mucins Glycoprotein Methods and Protocols Isolation ... UV monitor and collect fractions using a fraction col- lector and subject to the appropriate carbohydrate and antibody analyses. 3.6. Analysis of Mucins...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P2
... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... Rhodes, J. M. and Milton, J. D. (1986) Lectin Methods and Protocols, Humana, Totowa, NJ. 50. Liener, I. E., Sharon, N., and Goldstein, I. J. (1998) The Lectins: Properties, Func...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P3
... Methods and Protocols: The Mucins (Corfield, T. ed.), Humana, Totowa, NJ. 4. Nagma, K., Thornton, D. J., Khan, N., and Sheehan, J. K. (2000) Separation and identifi- cation of mucins and their ... sample volume, and superior wash- ing). For quantitation and comparison across blots, a standard in the same buffer as samples should be titrated for use as a standard curve, and samp...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P4
... will be greatly reduced and discontinuous. 7. Careful handling of the cryostat sections on the slide during fixing and staining is impor- tant because excessive washing and so on can remove mucus ... to rat, frog and human gastric mucosa: influence of feeding prostaglandin, N-acetyl-cysteine and other agents. Clin. Sci. 63, 187–195. 4. Bell, A. E., Allen, A., Morris, E. R., and Ro...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P5
... NJ 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. Introduction Most epithelial ... PhosphorImager. 4. The identification of mucin precursors and mature mucins using polypeptide-specific antisera and SDS-PAGE is elaborated in Chapters 20 and 21, and in several ref...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P6
... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... Separation and Identification of Mucin 77 7 Separation and Identification of Mucins and Their Glycoforms David J. Thornton, Nagma Khan, and John K. Sheehan 1. Introduction This ......
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P7
... K. E., and Chapman J. A. (1985) Mica sandwhich technique for preparing macromolecules for rotary shadowing. J. Ultrastruct. Res. 91, 66–76. 14. Kawahara K and Tanford C. (1966) Viscosity and density ... MUC5B, and MUC6 (1–4)—are gel-forming mucins, and it is possible that there are others. In isolating a mixture of mucins from mucus, there is consider- able scope for heterogeneity i...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P8
... caramelized and further charcoaled, and the acid hydrolysis results in a black residue. This residue appears to precipitate protein/amino acids, interferes with 114 Yan and Packer chromatography, and ... 2 min for pump and column equilibration with the initial composition of the mobile phase. 5. Figures 1 and 2 show typical chromatograms of the separation of amino acid standards...
Ngày tải lên: 08/10/2012, 10:25