cell growth, differentiation and senescence

... only by viable cells. By counting both blue and white cells, an estimate of cell viability can also be obtained. Percent viability = white cells/( blue cells + white cells) X 100%. Protocol ... the cell surface, such as cellular receptors. A 10-15 min incubation at 37°C is required to cause cell detachment and disrupt cell- cell adhesion.] 6. Trit...

Ngày tải lên: 10/04/2014, 11:13

... Cell Differentiation and Embryonic Development BIO101 - Bora Zivkovic - Lecture 2 - Part 2 There are about 210 types of human cells, e.g., nerve cells, muscle cells, skin cells, blood cells, ... between neurons and other neurons, muscles and glands. Those synapses that are used remain and get stronger, the other synapses detach, and the axons shrivel and die. Which brain cell...

Ngày tải lên: 26/12/2013, 01:18

... promotes growth activity of MGC803 cells. MGC803 cells were transfected with miR-23a ASO or pri-23a vector, and cell growth activity was detected through the MTT (A) and colony formation (B) assays ... Journal compilation ª 2010 FEBS 3731 Cell culture and transfection Human gastric adenocarcinoma cell line MGC803 was maintained in RPMI1640 (GIBCO BRL, Grand Island, NY, USA) supple...

Ngày tải lên: 18/02/2014, 04:20

... cell transfections and EGFP visualization Human HeLa cells were maintained in Dulbecco’s modified Eagle’s medium with 10% (v ⁄ v) fetal bovine serum and anti- biotics (100 UÆmL )1 penicillin and ... nurse cells of the ovaries and in the preblastoderm embryos [1]. After gastrulation, the maternal transcript disappears and the zygotic transcript and protein are found in a subset of...

Ngày tải lên: 19/02/2014, 05:20

... induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands: s, d, 2.5 and 20 m M choline; n, m, 2.5 mM and 20 mM atropine; h, j), 2.5 m M and 20 mM ... trigger cell lysis, formation of medium-length chains (6–10 cells on average) and visible alterations, such as cell bulges and Fig. 5. Effect of choline and analogs on the activ...

Ngày tải lên: 19/02/2014, 05:20

... IGF-1 on muscle cells, we used L6MLC ⁄ IGF-1 cells [23], a myogenic cell line in which IGF-1 expression is forced only after myoblast withdrawal from the cell cycle and commitment to differentiation. ... during myoblast differentiation and hypertrophy Alessandro Fanzani, Francesca Colombo, Roberta Giuliani, Augusto Preti and Sergio Marchesini Department of Biomedical Sciences an...

Ngày tải lên: 19/02/2014, 06:20

... relevance, as Notch controls cell differentiation in many tissues, regulating binary cell fate decisions and stem cell maintenance [51]. The development roles of pseudouridine synthases and their implications ... chosen, and their total wing area, anterior (A) and P compartments area and cell density were mea- sured (see Experimental procedures). Cell size was then estim...

Ngày tải lên: 06/03/2014, 22:21

... and temperature may also effective in increasing productivity. In this paper, we established cell suspension culture of ginseng cell and some attempts have been made to increase biomass and ... increase biomass and ginsenoside yield of ginseng cell culture. 2. Materials and methods Stock cell culture and culture condition Stock suspension cells of P. ginseng were...

Ngày tải lên: 14/03/2014, 10:20

... of EDS1 ⁄ PAD4 ⁄ SAG101 and BON1 ⁄ BAP1 + 2 in cell death and plant pathogen resistance. Whereas EDS1, PAD4 and SAG101 are positive regulators of cell death, BON1, BAP1 and BAP2 operate as negative ... membrane-bound and that their activa- tion during senescence may involve intra- and extra- cellular signals such as plant hormones and light [126]. A WRKY53 partner is EPITH...

Ngày tải lên: 16/03/2014, 02:20

... Daniels MA (2009) ERK and cell death: ERK location and T cell selection. FEBS J. 8 Martin P & Pognonec P (2009) ERK and cell death: cadmium toxicity, sustained ERK activation and cell death. FEBS ... establishment of senescence induced by a constitutively active form of Ras or MEK [121]. The Ras ⁄ Raf ⁄ ERK pathway and senescence Cellular senescence is an irreversi...

Ngày tải lên: 29/03/2014, 08:20