impact on cell proliferation differentiation and apoptosis

Ngày tải lên: 07/08/2014, 15:20

... expression was analyzed by the 2-ΔΔCT method Ca2+ concentration measurement Detection of ASMC proliferation The cells were divided randomly into groups: control group, substance P-induced, and WIN62577-induced ... control vs normal group remodeling Taken together, our results suggest that NK1R antagonist WIN62577 could decrease ASMC calcium concentration and inhibit ASMC proliferation and migration, and ... airway smooth muscle tone and secretions, bronchial circulation, and inflammatory and immune cells [10] Tachykinin levels were increased in induced sputum from asthmatic and cough patients with...

Ngày tải lên: 11/08/2014, 03:20

... expression was analyzed by the 2-ΔΔCT method Ca2+ concentration measurement Detection of ASMC proliferation The cells were divided randomly into groups: control group, substance P-induced, and WIN62577-induced ... control vs normal group remodeling Taken together, our results suggest that NK1R antagonist WIN62577 could decrease ASMC calcium concentration and inhibit ASMC proliferation and migration, and ... airway smooth muscle tone and secretions, bronchial circulation, and inflammatory and immune cells [10] Tachykinin levels were increased in induced sputum from asthmatic and cough patients with...

Ngày tải lên: 11/08/2014, 06:23

... Pulmonary function tests Specifics of pulmonary function testing in an older population The application of conventional quality control standards to objective assessment of pulmonary function ... describes the relationship between force of contraction (Pdi/Pdimax) and duration of contraction (ratio of inspiratory time to total respiratory cycle duration [TI/TTOT]) and is related inversely ... Brierley E, Johnson M, James O, et al Effects of physical activity and age on mitochondrial function Q J Med 1996;89:251 – Wilson DO, Rogers RM, Hoffman RM Nutrition and chronic lung disease...

Ngày tải lên: 05/03/2014, 21:20

... and DP9 overexpression to impair monolayer wound healing on ECM DP8 and DP9 impaired cell migration Cell migration was also assessed in transwells In vitro cell migration assays showed that cells ... integrin on activated cells [18] We recently showed that FAP overexpression in the LX-2 stellate cell line increases cell adhesion and migration and enhances induced apoptosis [12] DP9 contains ... adhesion deficit This concordance of apoptosis and adhesion suggests that one may cause the other In the proliferation studies we used cells transfected with V5–His fusion constructs and compared them...

Ngày tải lên: 23/03/2014, 11:20

... of credit policy, and the impact of a payment reporting system on operations, systems, and staffing 20 During the education process, Nicor found confusion among employees and consumers Many of ... communications, relations with regulators, public relations, implementation processes, and implementation costs and benefits Section 11 offers a summary and concluding thoughts on credit reporting ... Experian, one of the three large national credit bureaus, finds that consumers tend to choose to be late on some obligations and not on others rather than being late on all.7 The study was based on...

Ngày tải lên: 29/03/2014, 06:21

... fraction of cells in specific phases of the cell cycle and cells that have undergone cell death and DNA fragmentation and loss After various interventions, cells are harvested by trypsinization ... 243 244 245 247 Contents Markers of differentiation and markers of cessation of proliferation Morphology and related markers Enzymatic activities Evaluation of proliferation Cell cycle-associated ... Carbohydrate Analysis (2nd edition) Cell- Cell Interactions The Cell Cycle Cell Growth and Apoptosis if Cell Separation Cellular Calcium Cellular Interactions in Development Cellular Neurobiology * Chrornatin...

Ngày tải lên: 10/04/2014, 11:13

... http://www.jeccr.com/content/29/1/175 S-180 tumor cells and a panel of human tumor cell lines in vitro and the effect was mediated by inhibition of cell proliferation and up-regulation of programmed cell death ... camptothecin at mM concentration was 8.89% In contrast compound 1i at 10.0 and 16.7 mM concentrations was effective in inducing 27.54% and 30.86% apoptosis respectively The necrotic cell populations for ... turnover of cells leading to reduction of tumor cell number Analysis of apoptosis in MOLT-4 and HL-60 cells by Annexin V-FITC/PI double staining method MOLT-4 and HL-60 control and treated cells were...

Ngày tải lên: 10/08/2014, 10:20

... cell growth and proliferation 2.5 Effect of substrate stiffness on adhesion and cytoskeleton 2.6 Effects of stiffness of substrate on focal adhesion 2.7 Focal adhesion points in relation to cell ... background information on the wide biomedical applications of PCL and the cellular responses such as growth, proliferation and also focal adhesion contact points when cells are seeded onto a substrate ... adhesions on soft gel and static focal adhesion on stiff gels [Pelham, 1997] (b): Actin cytoskeleton on stiff and soft matrix [Discher, 2005] 2.7 Focal adhesion points in relation to cell proliferation...

Ngày tải lên: 28/09/2015, 13:28

... cell death and cell proliferation? When and why cells die? Does the concentration of environmental pollutants exert cytotoxic or cytostatic effects on cells? What factors influence the rate and ... studying apoptosis in individual cells 38 39 Cell Death – Apoptosis and Necrosis Cell Death – Apoptosis and Necrosis Apoptosis Assay Methods Apoptosis Assay Methods Cell Death – Apoptosis and Necrosis ... – Apoptosis and Necrosis Cell Death – Apoptosis and Necrosis Apoptosis Assay Methods Apoptosis Assay Methods Cell Death – Apoptosis and Necrosis Apoptosis Assay Methods Methods for studying apoptosis...

Ngày tải lên: 10/04/2014, 11:02

... Biotech Monitoring T cell division and early expansion using CFSE labeled cells To monitor cells division and expansion during the early days after stimulation, cells were CFSE-labeled and monitored ... anti-CD3-T cell receptor crosslinking on a surface, and the costimulation provided by cell- cell interaction between T cells and Fc receptor positive accessory cells such as monocytes which constitutively ... Li and Kurlander: Comparison of anti-CD3 and antiCD28-coated beads with soluble anti-CD3 for expanding human T cells: Differing impact on CD8 T cell phenotype and responsiveness to restimulation...

Ngày tải lên: 18/06/2014, 16:20

... effects of borax on immune cell proliferation (lymphocyte proliferation) and genotoxicity (sister chromatid exchange) We studied the toxic effect on immune cell proliferation by MTT assay and genotoxicity ... each borax concentration on immune cell proliferation (lymphocyte proliferation) by MTT assay and study about the genotoxicity effect were done by ANOVA test Figure The correlation between measured ... duplicate fashion Mean absorbance was calculate for the control wells and for each borax concentration in the test wells The degree of immune cell proliferation (lymphocyte proliferation) sensitivity...

Ngày tải lên: 20/06/2014, 00:20

... unit volume and the average cell size An increase in the blowing agent concentration resulted in smaller, finer, and more uniform cells The decomposition of high concentrations of carbon dioxide ... Relationship between force and displacement at different blowing agent concentrations Table 3: Effect of blowing agent concentration on the absorbed energy and toughness Blowing Agent Concentration ... Blowing Agent Concentration (phr) 12 Figure 2: Effect of blowing agent concentration on number of cells per unit volume Average Cell Size (mm) 1.5 0.5 10 12 Blowing Agent Concentration (phr) Figure...

Ngày tải lên: 07/08/2014, 14:20

... study; and manuscript preparation TM and HK contributed to MSC stimulation, quantitative PCR, and Bio-Plex; CIA induction and evaluation; humoral and cellular responses; and analysis of T -cell proliferation ... to isolation and characterization of MSCs; MSC stimulation, quantitative PCR, and Bio-Plex; CIA induction and evaluation; humoral and cellular responses; analysis of T -cell proliferation; design ... proliferation LG contributed to CIA induction and evaluation and to analysis of T -cell proliferation PM contributed to the design of the study and to manuscript preparation All authors contributed...

Ngày tải lên: 12/08/2014, 11:23

... analogue, on cell proliferation and p27kip1 expression, and examined the role of p27kip1 in HPASMC proliferation using p27kip1 gene silencing Methods Reagents We obtained reagents and materials ... effects of 8-Br-cAMP and BPS on p27kip1 expression were similar and p27kip1-dependent regulation of proliferation was confirmed in the p27kip1 knockdown cells Overexpression of p27kip1 in rat ... inhibition of CDK activity and in the proliferation of vascular smooth muscle cells [8,21-23] On the other hand, Li et al found that BPS suppresses systemic vascular smooth muscle proliferation through...

Ngày tải lên: 12/08/2014, 15:21

... fresh mononuclear cells were isolated by density centrifugation and plated in triplicate for mononuclear proliferation and in duplicate for cytokine assays Antigen-induced mononuclear cell proliferation ... Effects of winter birth season and prenatal cockroach and mouse allergen exposure on indoor allergen-specific cord blood mononuclear cell proliferation and cytokine production Ann Allergy Asthma Immunol ... Furthermore, antigen-specific maternal blood mononuclear cell proliferation was not correlated with antigen-specific age mononuclear cell proliferation (cockroach antigen n = 87, p = 0.82; mouse...

Ngày tải lên: 13/08/2014, 13:22

... action on T cells Other than induction of T cell proliferation and differentiation, T cell interactions with myeloid cells have been shown to induce T cell apoptosis Several studies show that T cell ... stimulation has been shown to inhibit T cell proliferation and to induce T cell apoptosis in vitro Further studies on CD4 T cells in the absence of CD137 showed enhanced CD4 T cell proliferation and ... immobilization on tissue culture plates 39 2.3 Cells and cell culture 40 2.3.1 Cell lines 40 2.3.2 Isolation of PBMCs, monocytes and T cells 40 2.3.3 In vitro generation macrophages of classical DCs and...

Ngày tải lên: 10/09/2015, 15:54

... functions of DMS and compounds on SPHK1 and SPHK2 activity A, Inhibition of SPHK1 activity by various concentrations of compounds and DMS is shown B, Inhibition of SPHK2 activity by various concentrations ... fact, in the concentrations tested (10μM, 25μM, 50μM, 75μM, and 100μM), none of the six compounds inhibited DAGK and human PKC alpha (Figure 2.8 and Figure 2.10) On the other hand, and in agreement ... Function on SPHK Activity in vitro 2.2.2.1 Over-expression of SPHK1 and SPHK2 2.2.2.1.1 EGFP-SPHK1 Plasmid Verification The EGFP-SPHK1 was constructed in the laboratory, and was amplified and purified...

Ngày tải lên: 11/09/2015, 16:06

... (hFOB) Cell Line The hFOB cell line is a clonal, conditionally immortalized human fetal cell line that is capable of osteoblastic differentiation and bone formation It was used as a positive control ... activity in the stem cells, the balance between proliferation and differentiation could be lost, the cell growth was arrested, and the cells would then enter into certain differentiation pathway(s) ... Human BM- and AD- MSCs Differentiation and limited numbers of the MSCs The culture conditions for these two differentiation pathways were addressed in Section 3.1.1.4 Stem Cells Osteogenic and Adipogenic...

Ngày tải lên: 11/09/2015, 16:06

... culture conditions Figure 4.7 S1P and PDGF-AB function in human BM- and AD- MSCs proliferation Human BM- and AD- MSCs were cultured in different culture conditions and cells proliferation were ... 10 cell growth 120% culture conditions 131 Chapter Role of S1P in human BM- and AD- MSCs proliferation Figure 4.6 Function of S1P and serum in human BM- and AD- MSCs proliferation Human BM -and ... DMEM+5uM S1P culture conditions Figure 4.5 Function of S1P in human BM- and AD- MSCs proliferation Human BMand AD- MSCs were cultured in different conditions for days, and cell growth was measured...

Ngày tải lên: 11/09/2015, 16:06