growth differentiation and survival

... and AgNOR References Cell growth and cytotoxicity assays Philip Skehan Introduction Growth and cytotoxicity assays Quantifying cell growth Experimental design Growth rate and doubling time Potency ... cytometric differentiation between G2 and M and between post-mitotic and pre-synthetic G1 (from ref 5) Equipment and reagents • • • BrdU PBS RNase A 0.1 M HCI PBST: ml ice-cold PBS and 0.4% Tween-20 ... Cell growth and kinetics in multicell spheroids 61 Ralph E Durand Introduction 61 Growth of spheroids Options Example: the V79 spheroid system 61 61 64 Special features of spheroids Metabolite and...

Ngày tải lên: 10/04/2014, 11:13

... accelerates muscle and motor neuron regeneration FASEB J 17, 53–55 Rosenthal SM & Cheng ZQ (1995) Opposing early and late effects of insulin-like growth factor I on differentiation and the cell cycle ... (1996) Growth hormone and the insulin-like growth factor system in myogenesis Endocrine Rev 17, 481–517 DeVol DL, Rotwein P, Sadow JL, Novakofski J & Bechtel PJ (1990) Activation of insulin-like growth ... primarily during embryogenesis and regeneration [28,29], IGF-1 has been reported to be essential for muscle differentiation and hypertrophy [1–3] Unlike other growth factors, IGF-1 is able to...

Ngày tải lên: 19/02/2014, 06:20

... association study from Finland and Sardinia shows the common variants in GDF5 contribute to height difference [33] The GDF5 gene might be involved skeletal growth and development and GDF5 variants might ... the design of the study and performed the statistical analysis TS and WW participated in its design and coordination and helped to draft the manuscript All authors read and approved the final ... et al: Large-scale analysis of association between GDF5 (rs143383) and FRZB (rs7775 and rs288326) variants and hip, knee and hand osteoarthritis Arthritis Rheum 2009, 60:1710-1721 Chapman K, Takahashi...

Ngày tải lên: 20/06/2014, 07:20

Ngày tải lên: 07/08/2014, 15:20

... their stem growth by 0.56 mm year Carten-Son et al [4] and Zhang -1 and Romane [34] found that oaks increased their stem growth in rainy late springs and summers In our study, annual growth of ... used two-way ANOVAs to test the effects of irrigation, shade and the interaction of irrigation and shade on seedling survival and growth For comparisons between treatment combinations, we used ... seedling growth and microclimate conditions in the different treatments For the correlations, n = 16 plots x years = 48 4 RESULTS 4.2 Seedling growth There 4.1 Seedling survival Irrigation, shade and...

Ngày tải lên: 08/08/2014, 14:21

... (top and middle left panels) and tumor growth in nude mice (bottom left) (B) In contrast, Trip10 overexpression in CP70 cells suppressed colony formation (right top and middle panels) and tumor growth ... both Cdc42 and huntingtin (HD) and shows cell type-specific localization Trip10 was cloned and transfected into (A) IMR-32 cells and (B) CP70 cells; individual colonies were selected and analyzed ... YWL and SHH designed the study and drafted the manuscript CCH, YWL, YLL and YCH carried out the MSP and bisulfite sequencing CCH carried out the ChIP PCR MJT cloned the human Trip10 TYK and JYY...

Ngày tải lên: 10/08/2014, 05:21

... normal growth, survival, and osmoregulatory function of crustaceans (Mantel and Farmer, 1983) When reared in potassium deficient seawater, L.vannamei displayed anorexia, low activity, poor growth and ... quartz and glass cm cuvettes, digital camera microscope, and others 3.3 Research design and experimental approaches 3.3.1 Experiment 1: Effects of Mg-P-K supplemented in water on growth and survival ... D.A., Saoud, I.P., Boyd, C.E., and Rouse, D.B., 2005 Effects of potassium, magnesium, and age on growth and survival of Litopenaeus vannamei postlarvae reared in inland low salinity well waters...

Ngày tải lên: 22/09/2015, 12:38

... reached highest body weight and shell height (0.33 g and 11.54 mm) compared to 600 ind./m2 (0,29 g and 10.29mm), 900 ind./m2 (0.23 g and 9.84 mm) and 1200 ind./m2 (0.21 g and 9.70 mm) At the stocking ... Treatment reached highest body weight and shell height (0.20g and 9.51mm) compared to treatment (0.19g and 9.14mm), treatment (0.18g and 9.05mm) and treatment (0.18g and 9.06mm), however there were ... snail .21 4.1.3 Growth rates of snails .23 4.1.4 Biomass growth rate and feed efficiency of snail 24 4.2 Effects of different diets on the growth and survival rate of Pila...

Ngày tải lên: 22/09/2015, 15:34

... growth and survival rates of cobia larvae in order to determine suitable level of probiotics 1.3 Content of the study Study on the effect of probiotics supplemented in water on the growth and survival ... of 14 probiotics on aquatic animal was observed by Douillet (1993) and Douillet and Langdon (1994) in enhancing survival and growth of Crassostrea gigas larvae The effect of probiotics containing ... (7a.m and 5p.m) Test kit Temperature times/ day (7a.m and 5p.m) Thermometer Nitrite Once a week (7a.m and 5p.m) Test kit TAN Once a week (7a.m and 5p.m) Test kit Nitrate Once a week (7a.m and 5p.m)...

Ngày tải lên: 18/11/2015, 22:22

... and vaguely lobulated Well developed fronds consist of epithelial-lined stromal projections that protrude into dilated glands or cystic spaces The glands are usually widely spaced, dilated, and ... pleomorphism, stromal overgrowth, tumor necrosis and heterologous stromal elements, or a combination of histological features to be prognostically useful On the other hand, several authors have ... that include the degree of stromal hypercellularity, stromal cytologic atypia and mitotic activity, stromal overgrowth, and circumscribed vs invasive margins [2] Although histological features have...

Ngày tải lên: 31/10/2012, 16:57

... Growth Performance and Sperm Quality of Stress Negative Piétrain Boars and Their Hybrids with Duroc Kawecka et al (2008), Wysokinska et al (2009), Pokrywka and Tereszkiewicz (2011) and Luc ... CO, MO and VOL RESULTS AND DISCUSSION 3.1 Growth performance The growth performance is influenced by the genetic group effect (P

Ngày tải lên: 28/08/2013, 11:59

... muscles, or glands, survive Other brain cells die Sometimes just parts of cells die off For instance, many more synapses are formed than needed between neurons and other neurons, muscles and glands Those ... muscles and glands Those synapses that are used remain and get stronger, the other synapses detach, and the axons shrivel and die Which brain cells and which of their synapses survive depends on their ... child The details of embryonic development and mechanisms of cell differentiation differ between plants, fungi, protists, and various invertebrate and vertebrate animals We will look at some...

Ngày tải lên: 26/12/2013, 01:18

... domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were generated by PCR and cloned into ... clarified the structural and functional importance of the v-KIND and MAP2 interaction core modules in the regulation of dendrite growth and branching in hippocampal neurons and cerebellar granule ... v-KIND and PTPN13 shared 46.9% homology and had only two or three conserved Leu residues out of seven residues It is notable that four Leu residues (amino acids 461, 474, 477 and 482) and one...

Ngày tải lên: 14/02/2014, 19:20

... scores and meta-analysis; and (d) end with an integrative summary and commentary that provides clinical and public policy implications and a look to the future The Key Studies Spiegel (2001) and ... Claims about survival benefits are often made using statistical techniques and interpretations that are unfamiliar to social and behavioral scientists Survival curves, slopes analysis, and proportional-hazard ... comply with PSYCHOTHERAPY AND SURVIVAL 371 Table Methodological Concerns and Consolidated Standards of Reporting Trials (CONSORT) Scores Investigator Methodological and analytical concerns CONSORT...

Ngày tải lên: 15/02/2014, 05:20

... c-h.heldin@licr.uu.se Platelet-derived growth factor (PDGF) and transforming growth factor-beta (TGF-beta) affect cell growth, survival and migration, and have important functions during the embryonal ... includes clonal expansion, differentiation, and long-term survival Incomplete differentiation has been reported, however, in particular for weak tumor antigens To understand the molecular basis ... mice revealed that DBP, HLF, and TEF regulate primarily the transcription of genes involved in hepatic, renal, and intestinal detoxification and genes of the innate and adaptive immune system REV-ERBalpha,...

Ngày tải lên: 19/02/2014, 07:20

... lymph nodes and surgical margins, and blood samples from patients and 868 controls Combining genomic and proteomic techniques, differences in gene and protein expression profiles from HNSCC and normal ... Acpc), and b-methylphenylalanine were designed and synthesized to obtained more potent and more selective l- and dopioid receptor ligands or higher stability against proteolytic enzymes Radioligand-binding ... function and survival OP-21 Caspase-dependent and geldanamycinenhanced cleavage of co-chaperone p23 in leukemic apoptosis G Gausdal1, B T Gjertsen2, K Fladmark1, H Demol3, J Vandekerckhove3 and S...

Ngày tải lên: 19/02/2014, 07:20

... nitrite/nitrate and VEGF-A levels on survival were evaluated Results: Serum nitrite/nitrate and VEGF-A levels of lung cancer patients and control group were 93.7 ± 48.9 and 63.7 ± 32.2 lM (P = 0.018), and ... CREB activation and its consequences on SMC migration and OPN regulation Stimulation of cultured SMC by UTP and PDGF-BB highly induces CREB activation via ERK1/2 and p38, and via p38 and PKA respectively ... molecules like growth factors (GF), ECM proteins and proteoglycans, and their ligands There is a close collaboration between GF’s and ECM in cell adhesion, proliferation, motility and invasion...

Ngày tải lên: 19/02/2014, 07:20

... concentration after stimulation with EGF and PMA together on the one hand, and the sum of the ERK-PP concentrations after stimulation with EGF and PMA separately on the other hand, was not constant, but increased ... NaCl/Pi and washed Fig Biphasic ERK-PP time profile induced by EGF or PMA alone and synergistic ERK phosphorylation induced by EGF and PMA together Cells were serum-starved for three days and subsequently ... Parker, P.J (1985) Autophosphorylation and protein kinase C phosphorylation of the epidermal growth factor receptor Effect on tyrosine kinase activity and ligand binding affinity J Biol Chem 260, 14538–...

Ngày tải lên: 19/02/2014, 16:20

... on See Brown, Gale, and Looney [2012a] See, for example, the critique by Feldstein [2012] and Brown, Gale and Looney’s[2012b] responses to their various critics 2 economic growth This is curious ... state and local taxes, I only include half the reported state and local taxes 9 Although both economic theory and historical experience suggest that a tax system with lower marginal rates and ... provide examples of how the growth effects of tax reform can depend on a variety of assumptions: Altig et al [2001], Diamond [2012], and Mankiw and Weinzierl [2005] Mankiw and Weinzierl’s main conclusion...

Ngày tải lên: 20/02/2014, 19:20

... pg/well) and the intra- and inter-assay coefficients of variation were less than 7% and 9% respectively The progesterone detection limit was 12 pg/tube (60 pg/ well), and the intra- and inter-assay ... proliferation and survival are clearly enhanced [13,14] From our results, both proliferation and survival of GC cultured on LN, unlike steroidogenesis, were closely associated with cell shape and cytoskeleton ... conceived the study, participated in its design and coordination, statistical analysis and interpretation of data and drafted the manuscript FLB, SF and DM read and approved the final manuscript CP died...

Ngày tải lên: 05/03/2014, 17:20