cell culture
Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx
... that the CHO cell culture assay system can be validly employed to characterize individual insect EcR–USP heterodimers for their responsiveness to agonists and potentiators. Utility of the cell culture ... CHO cell extracts expressing the EcR vectors used in this study as detected with 9B9 (LdEcR) and DDA2.7 (DmEcR) monoclonal anti- bodies, as described in the text. Extracts from cel...
Ngày tải lên: 18/02/2014, 08:20
Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt
... cells were obtained per 500 mL culture of 8-day-old +P i and –P i cells, respectively). Eight days following subculture of the tomato cells into –P i culture media, secreted (culture media) AP activity ... –P i cell culture being resupplied with 2.5 m M P i . All subsequent studies were performed using cell culture media filtrate from 8-day-old –P i cells. AP activity staining of...
Ngày tải lên: 23/03/2014, 21:20
basic cell culture protocols methods in molecular biology - cheryl d. helgason, cindy l. miller
... of cells cultured and the risk that these cells might contain, and transmit, infectious agents. For example, culture of primate cells, transformed human cell lines, mycoplasma-contaminated cell ... the control DNA as internal standard (b). Cell cultures A, C, and E are mycoplasma positive; cell culture B is mycoplasma negative. The analy- sis of cell culture D is not evaluable...
Ngày tải lên: 08/04/2014, 12:50
cancer cell culture, methods and protocols
... Langdon Basic Techniques of Cell Culture 21 2.4. Cloning 1. Cells. 2. Cell culture medium. 3. Trypsin-EDTA. 4. Hemocytometer or cell counter. 5. Petri dishes. 6. Agar. 2.5. Cell Counting 2.5.1. Hemocytometer ... increasing percentage of cells will undergo cell division. The initial cell density is a factor here and the higher the cell density, the more rapidly the culture...
Ngày tải lên: 10/04/2014, 11:11
cell culture
... problems of contamination of their cell cultures with other cell types (see this volume [2]). Frequently, cultures have become contaminated with HeLa cells; these cells replicate rapidly and 12 ... for growing mass cultures is clearly related to the particular cell strain or cell line to be used. If diploid, anchorage- dependent cells are employed, then monolayer cell culture...
Ngày tải lên: 10/04/2014, 11:12
epithelial cell culture protocols
... types of cells, including smooth muscle cells, mesenchymal cells, vessel forming cells, nerve cells, blood cells, including immune cells and gastric gland cells. Gastric epithelial cells can ... epithelial cells, plate cells onto collagen-coated membranes and culture as above (see Subheading 3.2.) (2). 7. To examine the repair and proliferation of epithelial cells, plate cells onto Millicel...
Ngày tải lên: 11/04/2014, 01:27
human cell culture, volume iv
... Progenitor Cells 15 Table 4. Advantages and disadvantages of the two major culture approaches Static CD34 - enriched Perfused cultures with cell cultures accessory cells Advantages Simple culture ... primitive cell assay is the long - term culture - initiating cell (LTC - IC) assay. This assay utilizes long - term culture (five to eight weeks) of cells on a BM stromal layer...
Ngày tải lên: 11/04/2014, 09:46