cardiac cell and gene transfer
cardiac cell and gene transfer
... Metzger Cardiac Cell and Gene Transfer Principles, Protocols, and Applications Gutted Adenoviral Vectors 21 line, such as 293 cells (13). We use the C7 cell line, which was derived from 293 cells and ... cell HUMANA PRESS Methods in Molecular Biology TM Edited by Joseph M. Metzger Cardiac Cell and Gene Transfer HUMANA PRESS Methods in Molecular Biology TM VOLUME 2...
Ngày tải lên: 10/04/2014, 11:11
components of cell and gene therapy
... deficiencies causing Tay-Sachs and Sandhoff disease. Oncogene Transfer to Neural Cells A variety of methods have been developed to generate cell lines from primary cells and developmental neurobiologists ... host tissue. Important advances that use primary cells, stem cells, and cell lines that withdraw from the cell cycle are 214 COMPONENTS OF CELL AND GENE THERAPY FOR NEU...
Ngày tải lên: 10/04/2014, 22:11
vascular disease, molecular biology and gene transfer protocols
... methodologies and limitations, in Genetics of Common Dis- eases (Day, I. N. M. and Humphries, S. E., eds.), BIOS Scientific Publishers, Oxford, pp. 33–74. 2. Cooper, D. N. and Krawczak, M. (1993) Human Gene ... Biology and Gene Therapy Protocols Edited by: A. H. Baker © Humana Press Inc., Totowa, NJ 2 Analysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex Analys...
Ngày tải lên: 11/04/2014, 10:39
Báo cáo khoa học: Malonyl-CoA decarboxylase (MCD) is differentially regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique potx
... regulated in subcellular compartments by 5¢AMP-activated protein kinase (AMPK) Studies using H9c2 cells overexpressing MCD and AMPK by adenoviral gene transfer technique Nandakumar Sambandam, Michael ... mito- chondrial and cytosolic fractions of these cardiac cells. Materials and methods H9c2 cell culture H9c2 cells (ATCC, Rockville, MD, USA) were grown as myoblasts to confluenc...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Expression profiling reveals differences in metabolic gene expression between exercise-induced cardiac effects and maladaptive cardiac hypertrophy pot
... genes were upregulated while 62 genes were downregulated. The upregulated genes represented 179 known genes, 66 expressed sequence tags (EST) and 22 replicate genes. Among the downregulated genes ... West- ern world and is commonly associated with cardiac hypertrophy. Sustained cardiac hypertrophy leads to cardiac dysfunction, heart failure, arrhythmia and sudden death. As a resul...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: Adeno-associated virus gene transfer in Morquio A disease – effect of promoters and sulfatase-modifying factor 1 pot
... & Mannoni P (2000) Gene transfer to hepatocellular carcinoma: transduction efficacy and transgene expression kinetics by using retroviral and lentiviral vectors. Cancer Gene Ther 7, 1286–1292. 57 ... epithelial cells. Hum Gene Ther 8, 575–584. 46 Stoneley M & Willis AE (2004) Cellular internal ribo- some entry segments: structures, trans-acting factors and regulation of...
Ngày tải lên: 29/03/2014, 21:20
gene transfer and expression protocols
... Unhealthy cells, such as mycoplasma-infected cells, or cells grown either to confluency or under suboptimal conditions, generally give poor results. 3. Some cells are sensitive to EDTA, and some ... Acetyltransferase Gene into Eukaryotic Cells Using Diethyl-Aminoethyl (DEAE)-Dextran RichardA Lake and Michael J. Owen 1. Introduction The study of gene regulation in eukaryotic ce...
Ngày tải lên: 11/04/2014, 09:42
plant gene transfer and expression protocols
... D R , and Howell, S H (1986) Transient and stable expression of the firefly luctferase gene m plant cells and transgemc plants Science 234, 856-859 18 Mullmeaux, P. M., Guermeau, F., and Accotto, ... Gritz, L. and Davtes, J (1983) Plasmid-encoded hygromycin B resistance* the sequence of hygromycm B phosphotransferase gene and its expression in Escherzchza colz and Saccha...
Ngày tải lên: 11/04/2014, 10:10
Báo cáo y học: "Gene Therapy: The Potential Applicability of Gene Transfer Technology to the Human Germline"
... include transgenesis, gene therapy and bioethics. He is an experienced review article writer and has published several excellent reviews in the fields of transgenesis, gene therapy and bioethics. ... significant and important advances in gene transfer technology. K K e e y y w w o o r r d d s s gene targeting; gene therapy; genetic modification; pronuclear micr...
Ngày tải lên: 03/11/2012, 10:01
MÔ HìNH HOá QUá TRìNH TRAO ĐổI NHIệT ẩM TRONG MáY ấP TRứNG GIA CầM Modeling of heat and mass transfer in chicken egg incubators
... CầM Modeling of heat and mass transfer in chicken egg incubators Nguyễn Văn Đờng 1 SUMMARY The relationships between temperature and humidity of the airflow, temperature and water content of ... of heat and mass transfer in the space containing eggs in a non-linear differential model . The model allowed to determine basic parameters of an incubators that are used for analyz...
Ngày tải lên: 28/08/2013, 08:31