... mMdithiothreitol and 10 mMMgCl2)usingamotorized mortar and pestle (Torsion Balance Co, Clinton,NJ, U SA). Following centrifugation to remove nuclei, yolkplatelets, mitochondria (10 000 g, 20 min) and ... thought to represent destabilizing elements leading to rapid deadenylation and messenger breakdown [47,48].Thus, while CLAP appears to be somewhat refractory to protease degr adation, its mRNAs ... loned i nto p CR2.1(Invitrogen, Burlington, ON, Canada). Approximately2 · 106plaques w ere screened using standard protoco ls[34], and six plaques, identi fied by h ybridization to the564...