membrane protein protocols - barry s selinsky

... Cells overexpressing this protein are broken by vigorous shaking with glass beads and a total membrane fraction is prepared from these cells for the characteriza- tion of full-length SR-BI by SDS-PAGE ... expressed in tissues other than brown adipose tissue and provide potential therapeutic targets for the treatment of metabolic diseases such as dia- betes and obesity and of inflammatory d...

Ngày tải lên: 08/04/2014, 12:51

... of metabolic diseases such as dia- betes and obesity and of inflammatory diseases (5–7). Hydropathy analysis of these 33-kDa membrane proteins proposes six trans- membrane domains, confirmed for ... Biology TM Methods in Molecular Biology TM Edited by Barry S. Selinsky Membrane Protein Protocols VOLUME 228 Expression, Purification, and Characterization Edited by Barry S. Seli...

Ngày tải lên: 11/04/2014, 09:50

... is a proline- and glycine-rich transmembrane protein, and may thus be classified as a simple sequence protein (SSP). This type of sequence is fre- quent in organisms having G + C-rich genomes, ... membrane proteins present on the bacillus and its host [8]. The M. tuberculosis cell membrane, and that of other prokaryotes, contains proteins func- tioning as enzymes, ligands, signal transd...

Ngày tải lên: 18/02/2014, 16:20

... RL-pS-PPSSSAASS-pY-SFSDLNSTR (aa res- idues 47–68), which is phosphorylated at S- 49 and Y-59. The serine phosphorylation site, unique for human emerin, reflects probably a basal phosphoryla- tion ... RL-pS-PPSSSAASS-pY-SFSDLNSTR (aa residues 47–68), which is phosphorylat- ed at S- 49 and Y-59, obtained by using the SILAC approach. In both figure parts, the phosphotyrosine-specific mass differ...

Ngày tải lên: 07/03/2014, 12:20

... proteins have suggested the pos- sibility of nonsequential insertion mechanisms, where interactions between neighboring transmembrane seg- ments or re-orientation of transmembrane segments during ... be that N-tails are so rapidly trans- located across the membrane that some chains pass OST too fast to be glycosylated and only become modified in a slower, post-translational process. The final...

Ngày tải lên: 07/03/2014, 16:20

... O, Stern DM, Hartmann E, Ogawa S & Tohyama M (1999) Stress-associated endoplasmic reticulum protein 1 (Serp1) ⁄ ribosome- associated membrane protein 4 (Ramp4) stabilizes membrane proteins ... the details of the mechanism remain unknown. PAT-10 was discovered as a translocon-associated protein dur- ing a search for Sec61 partners during opsin nascent chain insertion [50]. It i...

Ngày tải lên: 22/03/2014, 15:21

... cells (Fig. 8). Therefore, it was suggested that COS-7 cells secreted exosome-like membrane vesicles and that MFG-E8 was secreted as a complex with the exosome-like membrane vesicles. It was also ... that COMMA-1D cells endogenously expressed MFG-E8 -S and a small amount of MFG-E8-L mRNAs [9]. To clarify whether MFG-E8 expressed in COMMA-1D cells was secreted with membrane vesicles, th...

Ngày tải lên: 24/03/2014, 03:21

... analysis has been particularly useful for the study of genes that would be lethal in a homo- zygous mutant state; this approach also makes it possible to recover essential genes in mosaic screens ... Pax-1 in this fused somite is less than in the normal contra-lateral somites. (C) A hematoxylin-eosin stained horizontal section of a chick embryo displaying a Pax-1 antisense ODN-induced somite...

Ngày tải lên: 08/04/2014, 12:56

... differentiated tumors (17). 4. Anti-CD31 is commonly used for visualizing vascularization in tissue sec- tions (18,19). 5. In some tissues, expression of a determined adhesion molecule is associated with malignant ... alcohol; six-well culture plates; 11 × 22 mm glass coverslips (Thomas Scientific, Swedesboro NJ). 3. Tweezers for handling the coverslips. 2.2. Transfection of COS Cells on Cove...

Ngày tải lên: 10/04/2014, 22:13

... facilities necessary for handling of radioisotopes. 3.1. Assembly of the Flow-Dialysis Cell When everything is ready to begin, the flow-dialysis cell, which has been stored as disassembled parts, is ... cellulose dialysis tubing is prepared for use as a dialy- sis membrane. Select the appropriate membrane considering the rate of dialysis, as well as the pore size to keep proteins and effec...

Ngày tải lên: 11/04/2014, 00:23