enzyme kinetics and mechanisms

enzyme kinetics and mechanisms

enzyme kinetics and mechanisms

... steady-state enzyme kinetics and mathematical modeling has been a productive and happy one. One of the reasons for the rather happy marriage between steady-state enzyme kinetics and mathematical ... data and hypotheses from other techniques such as x-ray crystallography, fast-reaction kinetics, and others. Certain kinds of enzymes are difficult to study. Polymerase and de...
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Báo cáo khoa học: Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of b-lactam antibiotics pptx

Báo cáo khoa học: Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of b-lactam antibiotics pptx

... NIPGB, NIPAOB and p-NPA. For NIPGB and NIPAOB, the final enzyme concentration was 4 l M and substrate concentration was 10ÆK m . For p-NPA the final enzyme concentration was 3 l M and substrate ... kinetic properties of the enzyme and mutants [4,9–13], investiga- tions into the individual rate and binding constants underlying the kinetic properties and biocatalytic perform-...
Ngày tải lên : 08/03/2014, 08:20
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enzyme kinetics principles and methods

enzyme kinetics principles and methods

... Contemporary enzyme kinetics and mechanism. Academic Press, New York. Purich, D.L. (1999) Handbook of biochemical kinetics. Academic Press, New York. Piszkiewicz, D. (1977) Kinetics of chemical and enzyme- catalyzed ... of enzyme kinetics. Butterworth, London. Cornish-Bowden, A. & Wharton, C.W. (1988) Enzyme kinetics. IRL Press, Oxford. Cornish-Bowden, A. (1995) Fundamen...
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Tài liệu Kinematics and Mechanisms P2 docx

Tài liệu Kinematics and Mechanisms P2 docx

... F. 1935. Three thousand years of progress in the development of machinery and lubricants for the hand crafts. Mill and Factory. Vols. 16 and 17. Peachey, J., Van Alsten, J., and Granick, S. 1991. ... 123:113 −126. Bhushan, B. and Gupta, B. K. 1991. Handbook of Tribology: Materials, Coatings, and Surface Treatments. McGraw-Hill, New York. Bhushan, B., Israelachvili, J. N., and La...
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Tài liệu Kinematics and Mechanisms P1 pptx

Tài liệu Kinematics and Mechanisms P1 pptx

... design and analysis of moving devices (or mechanisms) and machinery and their components. Kinematic analysis is usually the first step in the design and evaluation of mechanisms and machinery, and ... methodologies for planar and spatial linkages to guide a body in a desired way are found in Mechanism Design: Analysis and Synthesis by George Sandor and Arthur Erdman and...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... seven a-helices, a three-stranded antiparallel b-sheet and loops connecting these secondary structure elements. The con- tents of the a-helix and b-strand were 50% and 11%, respectively. The monomer ... coordi- nated with six ligands: the five same ligands in the Mn-reconstituted enzyme and an additional water oxy- gen, which, together with the OD2 of Asp165, the NE2 of His79 and t...
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Tài liệu Báo cáo khoa học: Enzyme kinetics informatics: from instrument to browser pdf

Tài liệu Báo cáo khoa học: Enzyme kinetics informatics: from instrument to browser pdf

... home-produced enzymes have been assayed (see Materials and methods). A number of time series assays were acquired for each enzyme, and the data captured and analysed using the kineticswiz- ard. ... databases con- taining enzyme kinetics data that adhere to these recom- mendations, with BRENDA [6], a database for enzyme functional data, and the biochemical reaction kinetics da...
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Tài liệu Báo cáo khoa học: Simplified yet highly accurate enzyme kinetics for cases of low substrate concentrations ppt

Tài liệu Báo cáo khoa học: Simplified yet highly accurate enzyme kinetics for cases of low substrate concentrations ppt

... concentrations of enzymes and substrates disappears. This is also the case with enzymes acting on polynucleotides (such as DNA gyrase and ribosomes), where the concentrations of enzymes and binding ... k 2 , k )2 and p are the same as those shown in Fig. 1 and the total enzyme con- centration is e tot ¼ 0.2 in (A), e tot ¼ 4 in (B), and e tot ¼ 40 in (C). H. M. Ha ¨ rdin et al....
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Tài liệu Báo cáo khoa học: Models and mechanisms of O-O bond activation by cytochrome P450 A critical assessment of the potential role of multiple active intermediates in oxidative catalysis doc

Tài liệu Báo cáo khoa học: Models and mechanisms of O-O bond activation by cytochrome P450 A critical assessment of the potential role of multiple active intermediates in oxidative catalysis doc

... Boussac, A., Hildebrandt, P. & Girer, J.J. (2000) Fe III -hydroperoxo and peroxo complexes with aminopyridyl ligands and the reson ance Raman spectro scopic ide ntification of theFe-OandO-Ostretchingmodes.Eur. ... M., And ersson, L .A. & Dawson, J.H. ( 1982) S ulfur don or ligand binding to ferric cytochrome P450cam and myo globin. Ultraviolet-visible absorption, magnetic c irc...
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Tài liệu Báo cáo khoa học: Kinetics and thermodynamics of nick sealing by T4 DNA ligase pptx

Tài liệu Báo cáo khoa học: Kinetics and thermodynamics of nick sealing by T4 DNA ligase pptx

... of Biotechnology Centers in the Netherlands (ABON) (Project I.2.8) and in part by the Netherlands Research Council for Chemical Sciences (CW) with financial aid from the Netherlands Technology Foundation ... Structural and mechanistic conservation in DNA ligases. Nucleic Acids Res. 28, 4051–4058. 28. Fersht, A. (1999) Structure and mechanism in protein science. In A Guide to Enzyme Cat...
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