Enhanced production of pectinase by Bacillus sp DT7 using solid state fermentation docx

... Enhanced production of pectinase by Bacillus sp. DT7 using solid state fermentation Des Raj Kashyap a , Sanjeev Kumar Soni a , Rupinder Tewari b, * a Department of Microbiology, ... levels of alkaline and thermotolerant pectinase by solid state fermentation. Production of this enzyme was affected by nature of solid substrate, level of moisture co...

Ngày tải lên: 01/04/2014, 00:21

... of the targets of regulation by the kinase [3]. Recently it has been shown that deregulation of Cdc6, ORC (origin recognition com- plex) and Mcm, all of which are targets of phosphorylation by ... expression of Mcm genes in growth-arrested cells is induced by addition of serum [43]. There are several E2F binding sites in the promoter region of the Mcm5, 6,and7 genes, and...

Ngày tải lên: 20/02/2014, 23:20

... degradation or caspase-dependent degra- dation. Overexpression of p53 induces caspase-depen- dent cleavage of DNp63a [15] by an unknown mechanism. Ubiquitination, by comparison, occurs at steady state and ... destabilizes DNp63a by both proteasomal and caspase-dependent degradation pathways. The degradation of DNp63a by TNF-a was attenuated by inhibition of NF-jB, sug- ges...

Ngày tải lên: 16/03/2014, 06:20

... with integrated speech, two sources of information are available to recognize the speech input: the target language speech and the given source language text. The target language speech is a human-produced ... perplexity of the speech recogni- tion test corpus is about 83. The acoustic model of the ASR system can be characterized as follows: • recognition vocabulary of 16716 words; •...

Ngày tải lên: 20/02/2014, 12:20

... produce high-aspect-ratio microstructure Fabrication of high-aspect-ratio micro structures using 21 (HARM) for a variety of MEMS applications. Modifications and/or improvements of the UV lithography ... Thickness vs. spin speed curves for SU8 750 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 Spin Speed (rpm) Film Thickness (um) 0 50 100 150 200 250 Fabrication of h...

Ngày tải lên: 05/03/2014, 14:20

... by comparison with the enzyme isolated from pig kidney). Spectrophotometric assay of fructose-1,6- bisphosphatase activity The enzyme activity was determined spectrophotometrically at 30 °C by ... Jasco 600 spectrometer (Jasco, Eas- ton, MD) using the 720 software and a cuvette of 0.1 cm path length. Five scans of each spectrum were collected from 250 to 200 nm in increments of...

Ngày tải lên: 07/03/2014, 05:20

... energies of the G194P were lower than those of the WT enzyme (Table 2). As the structure of the G194P mutant become more stable than that of the WT enzyme, the rmsf values (calculated by the MDS) of ... (2010) Cotranslocation of methyl parathion hydrolase to the periplasm and of organophosphorus hydrolase to the cell surface of Escherichia coli by the Tat pathway and ice n...

Ngày tải lên: 15/02/2014, 01:20

... embryogenic calli of a deepwater indica rice variety, Vaidehi, by using the biolistic method of transformation. The presence and expression of the Bt gene in regenerated plants were confirmed by Southern ... hectares of flooded lands in Asia (the river basins of Ganges– Brahmaputra of India and Bangladesh, the Irrawaddy of Myanmar, the Mekong of Vietnam and Cambodia, and t...

Ngày tải lên: 23/03/2014, 22:20

... mutagen produced by chlorination of insecticide fenitrothion using a quadrupole GC-MS and a high-resolution GC-MS. From the mass spectra obtained by the GC-MS, the chemical structures of two unidentified ... (2C5M4NP) was obtained by custom order from Chemical Soft R&D Inc. (Kyoto, Japan). 20 mg of each of the chemicals is dissolved into 2 L of distilled water and 4...

Ngày tải lên: 05/09/2013, 10:15

... [28]. NO production was not induced by 10 l M TS alone in the absence of LPS/IFN. However, treatment with TS 24 h before the addition of LPS/IFN enhanced LPS/IFN-induced NO production about twofold ... production is caused by enhance- ment of iNOS protein induction. Effects of a-T and its derivatives on the enhancement by TS of LPS/IFN-induced NO production We compar...

Ngày tải lên: 22/02/2014, 04:20