Báo cáo Y học: Mitogen-activated protein kinase kinase negatively modulates ciliary neurotrophic factor-activated choline acetyltransferase gene expression pdf
Báo cáo y học: " Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression"
... and array analysis. For each array
sample, the RNA was prepared from the purified
thymocytes. The thymocytes were processed by using
the RNEasy Mini Kit (Qiagen, Valencia, CA). Quality
and ... Canonical pathways containing thymocyte genes which
changed expression levels with age. These pathways were
identified by the ingenuity data analysis program
(www.ingenuity.com) using our ... a...
Báo cáo Y học: Induction of chicken ovalbumin upstream promoter-transcription factor I (COUP-TFI ) gene expression is mediated by ETS factor binding sites doc
... (Bio-Rad).
Electromobility shift assay
For EMSA (electromobility shift assay) studies, proteins
were prepared (with or without [
35
S]Met) from different
DNA constructs using the TNT Coupled reticulocyte lysate
system ... some ETS factors in the mouse
embryo. These results indicate that members o f the ETS
family can activate COUP-TFI gene expression.
Keywords: COUP-TFI; ETS; gene expr...
Báo cáo Y học: Novel protein phosphatases in yeast docx
... regu-
lated and fulfil their func tions in the yeast cell.
Keywords: Ser/Thr protein phosphatases; cell cycle; cell
integrity; cation tolerance; protein synthesis; yeast.
INTRODUCTION
In a splendid cartoon ... other phenotypes described
below, is aggravated by deletion of PPZ2 [10]. Ppz1
functionally interacts with the protein kinase C-activated
mitogen activated protein (MAP) kinas...
Báo cáo y học: "HLA-DR regulation and the influence of GM-CSF on transcription, surface expression and shedding
... [1;2]. HLA-DR is a glycosylated cell surface transmembrane protein expressed
on antigen-presenting cells and constitutively expressed on monocytes. Expression of HLA-DR by
monocytes is essential ... 22-kDa glycoprotein cytokine secreted by mononuclear leukocytes including
monocytes. It functions as a growth and differentiation factor for immature phagocytic cells and
clinically it is...
Báo cáo Y học: Novel regulatory regions found downstream of the rat B29/Ig-b gene docx
... a
repressed gene during the expression of cell type-specific
genes. Yet all regions of this locus are reportedly always
relaxed in the human a-globin locus [38]. Thus, before cell
type-specific gene expression ... and analyze d on a 5% polyacryl-
amide sequencing gel.
RESULTS
Y3 cell-specific DNase I hypersensitive sites
B29/Ig-b mRNA was detected in rat plasmacytoma-derived
Y3 cells b...
Báo cáo Y học: Proliferating cell nuclear antigen from a basidiomycete, Coprinus cinereus Alternative truncation and expression at meiosis pptx
... ®ssion yeast gene encoding polymerase delta
accessory protein PCNA . EMBO J. 11, 5111±5120.
6. Xiong, Y. , Z hang, H . & Beach, D. (1992) D t ype cyclins a ssociate
with multiple protein kinases ... DNAs (pachytene) [8]. The former synthesis
was replication-type, and the latter was repair-type. These
observations indicate that accessory proteins of DNA
synthesis such as PCNA may ha...
Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt
... the
gap- and step-type HSEs. The different types of HSEs
result in the induction, by yeast HSFs, of different lev-
els of gene expression and probably in distinguishing
target genes by mammalian HSFs. ... cells, even at
physiological temperatures. The genes targeted by
HSF encode proteins that function in protein folding
and degradation, detoxification, energy generation,
carbohydrate me...
Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx
... transcription–PCR analysis of unknown serial analysis of gene
expression tags; rSAGE, reverse serial analysis of gene expression; SAGE, serial analysis of gene expression; TSAT-PCR, two-step analysis
of unknown ... types.
Abbreviations
GLGI, generation of longer cDNA fragments from serial analysis of gene expression tags for gene identification; PLF, primary library forward
pri...
Báo cáo khoa học: Effects of juvenile hormone on 20-hydroxyecdysone-inducible EcR, HR3, E75 gene expression in imaginal wing cells of Plodia interpunctella lepidoptera ppt
... Hiruma,K.,Shinoda,T.,Malone,F.&Riddiforf,L.M.(1999)
Juvenile hormone modulates 20-hydroxyecdysone-inducib le
ecdysone receptor and ultraspiracle gene expression in the tobacco
hornworm, Manduca s exta. Dev. Genes E vol. 209, 1 8–30.
22. ... cDNA pro duced by reverse transcription o f
the total RNAs. The degenerate primers ED1 5¢-forward
primer (5¢-AARTGYCARGARTGYMGNYT-3¢), ER1
5¢-re...
Báo cáo khoa học: Selenium affects biosilica formation in the demosponge Suberites domuncula Effect on gene expression and spicule formation pot
... homogeneous and, especi-
ally intracellularly, are associated with membranes and
fibrils, a high-resolution protein analysis of the axial fila-
ments is the only way to further identify (non)enzymatic
proteins ... This protein dis-
played no striking homology to any protein reported
in the database.
One selection criterion used to study this protein
was the existence of repeats; the spi...