Báo cáo Y học: Mitogen-activated protein kinase kinase negatively modulates ciliary neurotrophic factor-activated choline acetyltransferase gene expression pdf

... and array analysis. For each array sample, the RNA was prepared from the purified thymocytes. The thymocytes were processed by using the RNEasy Mini Kit (Qiagen, Valencia, CA). Quality and ... Canonical pathways containing thymocyte genes which changed expression levels with age. These pathways were identified by the ingenuity data analysis program (www.ingenuity.com) using our ... a...

Ngày tải lên: 03/11/2012, 11:35

... (Bio-Rad). Electromobility shift assay For EMSA (electromobility shift assay) studies, proteins were prepared (with or without [ 35 S]Met) from different DNA constructs using the TNT Coupled reticulocyte lysate system ... some ETS factors in the mouse embryo. These results indicate that members o f the ETS family can activate COUP-TFI gene expression. Keywords: COUP-TFI; ETS; gene expr...

Ngày tải lên: 17/03/2014, 17:20

... regu- lated and fulfil their func tions in the yeast cell. Keywords: Ser/Thr protein phosphatases; cell cycle; cell integrity; cation tolerance; protein synthesis; yeast. INTRODUCTION In a splendid cartoon ... other phenotypes described below, is aggravated by deletion of PPZ2 [10]. Ppz1 functionally interacts with the protein kinase C-activated mitogen activated protein (MAP) kinas...

Ngày tải lên: 17/03/2014, 23:20

... [1;2]. HLA-DR is a glycosylated cell surface transmembrane protein expressed on antigen-presenting cells and constitutively expressed on monocytes. Expression of HLA-DR by monocytes is essential ... 22-kDa glycoprotein cytokine secreted by mononuclear leukocytes including monocytes. It functions as a growth and differentiation factor for immature phagocytic cells and clinically it is...

Ngày tải lên: 03/11/2012, 09:57

... a repressed gene during the expression of cell type-specific genes. Yet all regions of this locus are reportedly always relaxed in the human a-globin locus [38]. Thus, before cell type-specific gene expression ... and analyze d on a 5% polyacryl- amide sequencing gel. RESULTS Y3 cell-specific DNase I hypersensitive sites B29/Ig-b mRNA was detected in rat plasmacytoma-derived Y3 cells b...

Ngày tải lên: 24/03/2014, 03:21

... ®ssion yeast gene encoding polymerase delta accessory protein PCNA . EMBO J. 11, 5111±5120. 6. Xiong, Y. , Z hang, H . & Beach, D. (1992) D t ype cyclins a ssociate with multiple protein kinases ... DNAs (pachytene) [8]. The former synthesis was replication-type, and the latter was repair-type. These observations indicate that accessory proteins of DNA synthesis such as PCNA may ha...

Ngày tải lên: 31/03/2014, 15:20

... the gap- and step-type HSEs. The different types of HSEs result in the induction, by yeast HSFs, of different lev- els of gene expression and probably in distinguishing target genes by mammalian HSFs. ... cells, even at physiological temperatures. The genes targeted by HSF encode proteins that function in protein folding and degradation, detoxification, energy generation, carbohydrate me...

Ngày tải lên: 18/02/2014, 04:20

... transcription–PCR analysis of unknown serial analysis of gene expression tags; rSAGE, reverse serial analysis of gene expression; SAGE, serial analysis of gene expression; TSAT-PCR, two-step analysis of unknown ... types. Abbreviations GLGI, generation of longer cDNA fragments from serial analysis of gene expression tags for gene identification; PLF, primary library forward pri...

Ngày tải lên: 07/03/2014, 04:20

... Hiruma,K.,Shinoda,T.,Malone,F.&Riddiforf,L.M.(1999) Juvenile hormone modulates 20-hydroxyecdysone-inducib le ecdysone receptor and ultraspiracle gene expression in the tobacco hornworm, Manduca s exta. Dev. Genes E vol. 209, 1 8–30. 22. ... cDNA pro duced by reverse transcription o f the total RNAs. The degenerate primers ED1 5¢-forward primer (5¢-AARTGYCARGARTGYMGNYT-3¢), ER1 5¢-re...

Ngày tải lên: 23/03/2014, 13:20

... homogeneous and, especi- ally intracellularly, are associated with membranes and fibrils, a high-resolution protein analysis of the axial fila- ments is the only way to further identify (non)enzymatic proteins ... This protein dis- played no striking homology to any protein reported in the database. One selection criterion used to study this protein was the existence of repeats; the spi...

Ngày tải lên: 30/03/2014, 20:20