... SAF34 and 8G8 for humans, SAF34, 8G8 and P4 for sheep,SAF34 and P4 for cattle, and SAF34 for mice; and for the C-ter-minal antibodies 6H4, SAF60 and SAF70 for humans, and mAbs6H4, SAF60, SAF70 ... mono- (j) and non-glycosylated (m) isoform were defined as 100% and the contribution of each bandwas calculated as percentage. Linearity inthe range of 4–10 lL of brain homogenateswas confirmed ... threePrPCprotein bands, the di- (d), mono- (j) and nonglycosylated iso-form (m), was analyzed using densitometry. The percentages of each band regarding to the total signal of PrPCwere calculated as arithmetic...