Báo cáo khoa học: "Position Specific Posterior Lattices for Indexing Speech" pot

... position information needed for recording a given word hit is not readily available in ASR lat- tices — for details on the format of typical ASR lattices and the information stored in such lattices the ... Sec- tion 4. 4 Position Specific Posterior Lattices As highlighted in the previous section, position in- formation is crucial for being able to evaluate prox- imity informati...

Ngày tải lên: 31/03/2014, 03:20

... as attractive potential targets for drug development [24,25]. Indeed, highly potent NH inhibitors could be very effective against protozoan infection. According to their substrate specificity NHs ... conditions for the expression of the enzyme were found to be when IPTG was added at A 600 = 3.0 and when the induc- tion was prolonged for 16 h. Therefore, these condi- tions were chosen fo...

Ngày tải lên: 18/02/2014, 17:20

... Science, Bangalore) for X-ray analysis, Dr A.G. Appu Rao and Dr Sridevi Annapurna Singh (Department of Protein Chemistry and Technology, CFTRI, Mysore) for CD measurements. A.B.V.K. thanks CSIR (New Delhi) for ... enzymatic hydrolysis. The former is harsh, time consuming, modifies the products and forms a large quantity of monomers [6]. In contrast, enzymatic hydrolysis produces specific...

Ngày tải lên: 19/02/2014, 12:20

... Shimoyama for their advice on the kinetic analysis and assistance with fluorescence microscopy observations. We also thank Dr S. Kanai and Ms N. Inoue of PharmaDesign, Inc., Japan for bio-informatic ... one of the frequent foreign materials for attachment in the molecular system of the holdfast. The barnacle is a unique sessile crustacean. Once the larva has settled on the foreign substr...

Ngày tải lên: 07/03/2014, 05:20

... primers. For NKA8: forward, 5¢-GTCTCCAGACGTCTCGAAC-3¢; reverse, 5¢-CATCCAAGTCTTGGGAGCTC-3¢. For NKA48: forward, 5¢-GATGCTAACTTCAGCGGAAAC TC-3¢; reverse, 5¢-CACGATGATGGATGAAATGGCG TC-3¢. For NKA49: forward, ... 5¢-CTCTCCAACACATGCTGACG TAG-3¢. Cycling conditions were 94 °C for 2 min, followed by 35 cycles of 94 °C for 30 s, 54 °C for 30 s, 72 °C for 2 min, and 72 °C for 10 min....

Ngày tải lên: 07/03/2014, 12:20

... m M ,KCl 3m M )for4 · 5 min. Fixation was performed with 2.5% paraformaldehyde in NaCl/P i for 20 min at room tempera- ture. Cells werewashed again with NaCl/P i for 4 · 5min.No permeabilization was performed. ... odorant, a new coverslip must be used for each odorant stimulation and for each concentration. Measure- ments were performed several times at each concentration and for e...

Ngày tải lên: 08/03/2014, 02:21

... proteins are essential for eukaryotic DNA replication and are the most likely candidates for the replicative DNA helicase responsible for unwinding DNA at the replication forks [1–3]. Consistent ... proteins, cells were immersed in buffer containing Triton X-100 used for cell fractionation and then incubated at 37 °C for 15 min in the same buffer before fixation (Figs 9 and 10). Cell...

Ngày tải lên: 16/03/2014, 13:20

... dibutyryl- cAMP and 0.1 mm IBMX for 2 h before the start of the experiment. All solutions used for hormone-treated cells contained 100 nm dexamethasone. After exposure for 4 min at room temperature ... cells with glucocorticoids. Only forkhead box A was exclusively present in hepatoma cells, and therefore appears to be an important determinant of the observed tissue specificity of carbam...

Ngày tải lên: 23/03/2014, 10:20

... preS region, with specific affinity for human hepatocytes, is localized in the N-terminus. The S region following the preS has two trans- membrane regions responsible for the formation of particles. ... tissue -specific targeting method is the latest area of focus. Although, viral vectors, such as those utilizing adenovirus or adeno-associated virus, have been devel- oped for gene thera...

Ngày tải lên: 23/03/2014, 15:20

... temperature for the indicated periods and immunoblotted (left two lanes). Purified MIG-17–6His, including both the proform and the activated form, was immunoblotted (right lane). Single asterisk, proform ... symmetrical U-shaped arms formed by direc- tional migration of gonadal leader cells called distal tip cells (DTCs) (Fig. 1B). The DTCs are formed at the anterior and posterior ends of...

Ngày tải lên: 30/03/2014, 04:20