Báo cáo khoa học: Mapacalcine specifically blocks hypoxia-induced calcium influx in rat hepatocytes potx
Báo cáo khoa học: Mapacalcine specifically blocks hypoxia-induced calcium influx in rat hepatocytes potx
... the
ischaemia-induced calcium in ux. We demonstrate here
that: (a) there are specific receptors for mapacalcine in rat
hepatocytes; (b) Mapacalcine is able to specifically block
ischaemia–induced calcium in ux ... Mapacalcine specifically blocks hypoxia-induced calcium in ux
in rat hepatocytes
Dominique Crenesse
1
, Ghislaine Neuilly
2
, Jean Gugenheim
3
,...
Tài liệu Báo cáo khoa học: Substrate specificity of the pseudouridine synthase RluD in Escherichia coli doc
... flip-
ping of three bases including the substrate uridine 32.
These structural elements are absent in normal tRNA.
RluA appears to recognize its substrate by indirect
readout of a protein induced ... 20
uridine residues in 23S rRNA and one out of eight uri-
dine residues in 16S rRNA to pseudouridine [9].
We have analyzed the substrate specificity of the
pseudouridine synthase RluD in vi...
Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... concentrations). The phyto-
alexin that displayed an inhibitory effect was further
examined in order to determine the type of inhibition
and the K
i
using brassinin (0.10–0.40 mm) as a sub-
strate in ... detoxify
brassinin (1) via hydrolysis to indolyl-3-methanamine
(3) [14]. Assays using cell-free homogenates incubated
with brassinin (1) demonstrated that the putative
hydrolase was induc...
Tài liệu Báo cáo khoa học: Pyrimidine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc
... acid in H
2
O. The
retention times of adenosine and adenine, inosine and hyp-
oxantine, guanosine and guanine, uridine and uracil, cyti-
dine and cytosine were 12.2 and 6.2 min, 10.5 and 4.7 min,
15.2 ... CaCl
2
(data not shown), indi-
cating that Ca
2+
is required in maintaining the active
site structure.
Substrate specificity and kinetic characterization
With the aim of gaining insight o...
Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx
... bead
characteristics, in which increases in molecular mass and
DA resulted in efficient loading and minimization of the
release of entrapped lipase [30]. The observed increase in
specific activity with increasing ... larger
d-spacing as a result of increase in the unit cell dimension
that results in the incorporation of fewer water molecules.
Decrease in the intensity of 020 reflec...
Báo cáo khoa học: Calcite-specific coupling protein in barnacle underwater cement docx
... materials in
10 min at 25 °C was evaluated by measuring the decrease in pro-
tein amount remaining in the solution. Adsorption to (A) calcite in
ASW, (B) glass in ASW, (C) benzoguanamine–formaldehyde ... benzoguanamine–formaldehyde resin
in ASW, (D) zinc oxide in ASW, (E) magnetite in ASW, (F) gold in
ASW, (G) polystyrene in ASW, and (H) calcite in pure water. Error
bars...
Báo cáo khoa học: Site specificity of yeast histone acetyltransferase B complex in vivo pot
... treatment.
To investigate whether HU induces an increase of
the Hat1 protein, we used a yeast strain expressing a
hemagglutinin (HA)-tagged version of Hat1. The
Hat1–HA protein level did not increase ... are
incorporated in trans during reassembly of the yeast
PHO5 promoter. Mol Cell 19, 279–285.
41 Linger J & Tyler JK (2006) Global replication-indepen-
dent histone H4 exchange in bud...
Báo cáo khoa học: Stage specific expression of poly(malic acid)-affiliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx
... a distinct fragmin-like actin-binding
protein that controls in vivo phosphorylation of actin by
the actin-fragmin kinase. Eur J Biochem 265, 240–250.
37 Donze O & Picard D (2002) RNA interference ... NKA48 contain only a single domain fold.
The stability of these two proteins is highly dependent
on calcium binding [25]. The typical domain motives
contain a ‘tyrosine corner’ in the d...
Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
... amino acid side chain was shown to be
a critical determinant for substrate interaction with PAT2.
Only glycine and alanine were a ble to induce comparable
inward currents in oocytes expressing ... substrate spectrum that
includes not only t he amino acids glycine, alanine, serine
and proline but also osmolytes such as sarcosine and
betaine, and the
D
-enantiomers of serine and alanine. ......
Báo cáo khoa học: Ligand-specific dose–response of heterologously expressed olfactory receptors potx
... odorant–receptor
interactions depending on odorant chain length, within the
putative ligand-binding site determined by transmembrane
domains IV–VII, which will be further investigated by
bio-informatic docking ... excitation) in the absence of
calcium, and F
s
is the 510 nm emission signal (380 nm
excitation) with a saturating concentration of calcium. Z1
and Z2 are the intrinsic fluoresce...
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