Báo cáo khoa học: Functional assembly of thylakoid DpH-depen

Báo cáo khoa học: Functional assembly of thylakoid DpH-dependent/Tat protein transport pathway components in vitro ppt

... 7). Twin arginine precursor binding by in vitro assembled  700 kDa complex As a ﬁrst test of the functionality of in vitro inserted components, we examined the ability of complexes containing in ... structure–function relationships of the individual components. Thylakoids are particularly amenable to in vitro incorporation of proteins. Not only are proteins integr...

Ngày tải lên : 30/03/2014, 20:20

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Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc

... molybdopterin cytosine dinucleotide form of the molybdenum pyranopterin cofactor; Mo-MGD, molybdopterin guanine dinucleotide form of the molybdenum pyranopterin cofactor; MPT, molybdopterin; Qor, quinoline ... of CMP and AMP (from FAD). GMP was not present in any Qor extract, indicating that the host strains did not incorporate Mo-MGD, or free GMP, into the cofactor binding domain...

Ngày tải lên : 17/03/2014, 10:20

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Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx

... that the N-terminal domain is involved in protein protein interactions with other hydrophilic domains of neighboring integral membrane subunits, and interspecies interactions are incompatible. From ... [15–18]. In the bovine complex a large subdomain is made up of  20 integral membrane proteins contributing > 60 transmem- brane segments. Some of these must be intimately involv...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... concentration of the 16 kDa protein subunit) increased linearly with increasing fractional saturation of the metal site in Bxe_A2876, the k cat determined from the molar concentration of Fe 2+ -containing ... cupin protein featuring a three-histidine metal site. To examine the unknown function of Bxe_A2876, we performed a detailed biochemical characterization of the recombinan...

Ngày tải lên : 18/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity docx

... quantities of protein util- izing the principle of protein dye binding. Anal Biochem 72, 248–254. 22 Knecht W, Bergjohann U, Gonski S, Kirschbaum B & Lofﬂer M (1996) Functional expression of a ... use in gene therapy. Abbreviations ACV, 9-(2-hydroxyethoxymethyl)-guanine; AraA, 9-(b- D-arabinofuranosyl)-adenine; AraC, 1-(b-D-arabinofuranosyl)-cytosine; AraT, 1-(b- D-arabinofur...

Ngày tải lên : 19/02/2014, 02:20

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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... C-terminal region, including the PAS-B domain of BMAL1, is required for the binding to DEC1, Fig. 4. Identiﬁcation of the domain of D EC1 that interacts with BMAL 1. Interactions of various DEC1 ... control protein. Fig. 6. Identiﬁcation of t he domain of B MAL1 that inte racts with DEC1. Interactions of various truncated BMAL1 mutants with DEC1 in NIH3T3 cells were examined...

Ngày tải lên : 19/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in ﬁbrinolysis and plasmin-induced cell detachment and apoptosis docx

... binding sites of kringles modeled after the structure of kringle 1 of prothrombin. Proteins 3, 85–96. 20 Thorsen S (1975) Differences in the binding to fibrin of native plasminogen and plasminogen modified ... lysine-binding site (LBS) of human plasminogen. Role of LBS in the regulation of fibrinolysis. Eur J Biochem 157, 65–69. 48 Brockway WJ & Castellino FJ (1972) Measure...

Ngày tải lên : 20/02/2014, 01:20

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Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... the N-terminal helices 1 and 2 while leaving the interacting a-helix 8 intact, may destabilize the proteins leading to spurious protein protein or/and protein DNA interactions. The crystal structure of ... recombination could be instrumen- tal in resolving concatamers into linear monomers in the process of mtDNA partitioning and transmission into buds [11]. Several groups identiﬁed...

Ngày tải lên : 20/02/2014, 11:20

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Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

... are known to be involved in either protein protein interactions [61,62] or protein RNA interactions [63]. The proposed intramolecular interaction may be an integrated step in the guanine nucleotide-exchange ... translation indirectly by inhibition of mRNA synthesis during amino acid starvation [20–23]. Kirromycin is one in a family of antibiotics that inhibits protein synthesis...

Ngày tải lên : 21/02/2014, 00:20

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Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx

... N-terminal region. The results obtained indicate that, although the substrate-binding site is located in the C-terminal domain, the E. coli Pta N-terminal domain is involved in stabilization of ... containing the last 326 amino acids in the case of Pta-F1, the last 352 amino acids in the case of Pta-F2, and the last 470 amino acids in the case of Pta-F3 (Fig. 2). PCR reactio...

Ngày tải lên : 06/03/2014, 11:20

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