Báo cáo khoa học: Functional assembly of thylakoid DpH-dependent/Tat protein transport pathway components in vitro ppt

... 7). Twin arginine precursor binding by in vitro assembled  700 kDa complex As a first test of the functionality of in vitro inserted components, we examined the ability of complexes con- taining in ... structure–function relationships of the individual components. Thylakoids are particularly amenable to in vitro incorporation of proteins. Not only are proteins integr...

Ngày tải lên: 30/03/2014, 20:20

... molybdopterin cytosine dinucleotide form of the molybdenum pyranopterin cofactor; Mo-MGD, molyb- dopterin guanine dinucleotide form of the molybdenum pyranopterin cofactor; MPT, molybdopterin; Qor, quinoline ... of CMP and AMP (from FAD). GMP was not present in any Qor extract, indicating that the host strains did not incorporate Mo-MGD, or free GMP, into the cofactor binding domain...

Ngày tải lên: 17/03/2014, 10:20

... that the N-terminal domain is involved in protein protein interactions with other hydrophilic domains of neighboring integral membrane subunits, and interspecies interactions are incompatible. From ... [15–18]. In the bovine complex a large subdomain is made up of  20 integral membrane proteins contributing > 60 transmem- brane segments. Some of these must be intimately involv...

Ngày tải lên: 19/02/2014, 16:20

... concentration of the 16 kDa protein subunit) increased linearly with increasing fractional saturation of the metal site in Bxe_A2876, the k cat determined from the molar concentration of Fe 2+ -containing ... cupin protein featuring a three-histidine metal site. To examine the unknown function of Bxe_A2876, we performed a detailed biochemical characterization of the recombinan...

Ngày tải lên: 18/02/2014, 06:20

... quantities of protein util- izing the principle of protein dye binding. Anal Biochem 72, 248–254. 22 Knecht W, Bergjohann U, Gonski S, Kirschbaum B & Loffler M (1996) Functional expression of a ... use in gene therapy. Abbreviations ACV, 9-(2-hydroxyethoxymethyl)-guanine; AraA, 9-(b- D-arabinofuranosyl)-adenine; AraC, 1-(b-D-arabinofuranosyl)-cytosine; AraT, 1-(b- D-arabinofur...

Ngày tải lên: 19/02/2014, 02:20

... C-terminal region, including the PAS-B domain of BMAL1, is required for the binding to DEC1, Fig. 4. Identification of the domain of D EC1 that interacts with BMAL 1. Interactions of various DEC1 ... control protein. Fig. 6. Identification of t he domain of B MAL1 that inte racts with DEC1. Interactions of various truncated BMAL1 mutants with DEC1 in NIH3T3 cells were examined...

Ngày tải lên: 19/02/2014, 16:20

... binding sites of kringles modeled after the structure of kringle 1 of prothrombin. Proteins 3, 85–96. 20 Thorsen S (1975) Differences in the binding to fibrin of native plasminogen and plasminogen modified ... lysine-binding site (LBS) of human plasminogen. Role of LBS in the regulation of fibrinolysis. Eur J Biochem 157, 65–69. 48 Brockway WJ & Castellino FJ (1972) Measure...

Ngày tải lên: 20/02/2014, 01:20

... the N-terminal helices 1 and 2 while leaving the interacting a-helix 8 intact, may destabilize the proteins leading to spurious protein protein or/and protein DNA interactions. The crystal structure of ... recombination could be instrumen- tal in resolving concatamers into linear monomers in the process of mtDNA partitioning and transmission into buds [11]. Several groups identified...

Ngày tải lên: 20/02/2014, 11:20

... are known to be involved in either protein protein interactions [61,62] or protein RNA interactions [63]. The proposed intramolecular interaction may be an integrated step in the guanine nucleotide-exchange ... translation indirectly by inhibition of mRNA synthesis during amino acid starvation [20–23]. Kirromycin is one in a family of antibiotics that inhibits protein synthesis...

Ngày tải lên: 21/02/2014, 00:20

... N-terminal region. The results obtained indicate that, although the substrate-binding site is located in the C-terminal domain, the E. coli Pta N-terminal domain is involved in stabilization of ... containing the last 326 amino acids in the case of Pta-F1, the last 352 amino acids in the case of Pta-F2, and the last 470 amino acids in the case of Pta-F3 (Fig. 2). PCR reactio...

Ngày tải lên: 06/03/2014, 11:20