Báo cáo khoa học: Bilayer localization of membrane-active peptides studied in biomimetic vesicles by visible and fluorescence spectroscopies pptx

... FEBS 2003 Bilayer localization of membrane peptides (Eur. J. Biochem. 270) 4487 Bilayer localization of membrane-active peptides studied in biomimetic vesicles by visible and fluorescence spectroscopies Tanya ... (Figs 2 and 5, and Table 1). Melittin and magainin, however, reduced the diffusion rate of Rhodamine Red-DHPE (Table 2). This result confir...

Ngày tải lên: 30/03/2014, 20:20

... myosin is low unless it is bound to actin. Actin activation is inhibited by the regulatory proteins tropomyosin, troponin T, troponin I and troponin C, which bind along actin fila- ments of skeletal ... the measured effects of Ca 2+ on binding in the presence of ATP [35,36], equilibrium binding in the presence of ADP [16], binding kinetics [15,37] and the general features of...

Ngày tải lên: 07/03/2014, 09:20

... quantitation of microgram quantities of protein uti- lizing the principle of protein-dye binding. Anal Bio- chem 72, 248–254. 33 Glantz SA (1988) Primer of Biostatistics. McGraw-Hill Inc., NY. Proline-rich ... mgÆmL )1 bacitra- cin), followed by centrifugation at 12 000 g for 20 min at 4 °C. Protein samples were denatured at 100 °C for 5 min in a buffer containing 1% SDS and 1%...

Ngày tải lên: 18/02/2014, 08:20

... phosphorylation of proteins involved in the trafficking of the receptor, including c-Cbl, could account for its intracellular retention. By contrast, TDI mutations in the ECD or disruption of the termination ... staining of 293-VnR and ATDC5 cells expressing the Y373C mutant with anti- FGFR3 and anti-phosphotyrosine sera showed both intracellular and cell-surface phosphotyr...

Ngày tải lên: 19/02/2014, 00:20

... decreases in intracellular spermine pools and increased spermidine levels in SMO1 and SMO5 cells. These changes in spermine and spermidine levels in both SMO1 and SMO5 overexpressing cell lines were ... microscopy of immunofluorescent staining of SMO in cells overex- pressing each of the isoforms provided direct visuali- zation and confirmation of protein localizati...

Ngày tải lên: 07/03/2014, 05:20

... lysosomal marker protein LAMP1, a finding that is in agreement with our in vitro binding data. Targeting of GLUT8 in the absence of AP1 and AP2 In order to investigate the contribution of AP adap- tors, ... (TAC) protein (interleukin-2 receptor a chain) targeted the protein to intracellular membranes, indicating that its N-terminal dileucine signal is sufficient for en- dosomal...

Ngày tải lên: 16/03/2014, 02:20

... nature of the huge cluster of MukBEF? MukB and MukEF form various heteroge- neous closed ring-like structures, and these condensin rings may be opened and closed as a result of ATP binding and hydrolysis ... proteins. MukE and MukF are two non- SMC subunits that together form an elongated frame structure in which the C-terminal winged-helix domain (C-WHD) of MukF is linked...

Ngày tải lên: 23/03/2014, 05:22

... sites of action of these polypeptides, C-terminal fusions of Sfh-proteins with yEGFP were constructed and the localization of the proteins in the yeast cell was determined. The enzyme catalyzing ... generated integra- tive versions of C-terminal yEGFP fusions in frame with the ORF of interest. Thus, yEGFP fusions were present in single copy in the genome and driven...

Ngày tải lên: 23/03/2014, 18:20

... leucine degradation and the sku mutant accumulates branched-chain amino acids in haemolymph, this mutant may be useful in the investigation of unique branched-chain amino acid catabolism in insects. Abbreviations ACAD, ... mechanism bypassing branched-chain a-keto acid dehydrogenase irreversibility, which enables indirect accumulation of upstream leucine in the sku mutant. It is...

Ngày tải lên: 18/02/2014, 04:20

... in membrane inter- action. The length of the N-terminal hairpin in HtA is intermediate between those in RNse T1 and a-sarcin, having 20 amino acids in HtA, 26 in a-sarcin and 12 in RNase T1. ... ribosomes producing the a fragment [1,28]. Recently, the impor- tance of the N-terminal hairpin and loop 2 of ribotox- ins in protein functionality and protein–protein and...

Ngày tải lên: 18/02/2014, 08:20