Báo cáo khoa học: Redox-regulated affinity of the third PDZ domain in the phosphotyrosine phosphatase PTP-BL for cysteine-containing target peptides ppt

... measuring the induction of chitin synthesis in the cultured integument of C. suppressalis larvae; the 50% effective concentration (EC 50 ) for the induction of the chitin synthesis in the cultured ... not located in the C-terminus of the D region of CsEcR, but in the middle part [31]. Although the role of these five amino acids is still unknown, the...

Ngày tải lên: 23/03/2014, 21:20

... study, employing soluble forms of the receptor [25,26,34], indicates that the ectodomain of the receptor is capable of dimer forma- tion in the absence of ligand. Affinity cross-linking of [ 125 I]IGF-II ... Seurin D, Segovia-Quinson B, Hardouin S & Binoux M (1986) Analysis of serum insulin-like growth factor binding proteins using western blotting: use of the m...

Ngày tải lên: 18/02/2014, 08:20

... probably form part of the copper binding sites in this protein. Although there are four histidines in the C-terminal, another histidine is present at position 18, and given the folded state of the ... methods for deter- mining the affinity of a ligand for a protein is iso- thermal titration calorimetry (ITC). We used ITC to determine the affinity of copper for...

Ngày tải lên: 19/02/2014, 06:20

... GnT-IV abolished the binding affinity (105, 204). However, addition of the bisecting GlcNAc (104) abolished the binding in the case of LSECtin. The specificity of the DC-SIGN- related lectins to agalactosylated ... as the ratio of fluorescence intensity relative to that obtained for agalactosylated aAGP in the absence of competitors. In the presence of eit...

Ngày tải lên: 15/03/2014, 23:20

... being involved in PDZ domain interactions [19]. For instance, the phenolic ring of tyrosine P )7 of the ErbB2 protein enters into a pocket formed by the extended b2–b3loop of the Erbin PDZ domain ... other residues are also important in determining specific- ity. In this study, we analysed the binding specificity of the third PDZ domain of protein tyr...

Ngày tải lên: 30/03/2014, 20:20

... respect to the function of the integrated ATPase network of the active striated muscle cell in which spe- cific CK isoforms are absent. The role of mitochondria in the ATPase network of the cell ... develop- mental consequences of deleting creatine kinase isoenzymes from the heart. Reaction kinetics of the creatine kinase isoenzymes in the intact heart. J Bi...

Ngày tải lên: 30/03/2014, 15:20

... at the A surface passing through the hole on the other side of the helix and the other at the D surface passing through the C surface on the other side of the helix. Table 1. Mutations of the ... 2007 The Authors Journal compilation ª 2007 FEBS The location of the C-terminal region on the inside of the helix immediately suggests a reason for the fai...

Ngày tải lên: 19/02/2014, 00:20

... making use of the fact that hydrophobins con- tain eight cysteines forming four disulfide bonds. Reduction of the four disulfides with dithiothreitol increased the masses of all peaks corresponding ... from H. jecorina, were used to identify further proteins with similar domains, and finally all hypothetical proteins encoding hydrophobins from the annotated genomes of the Broad...

Ngày tải lên: 19/02/2014, 02:20

... in the region of the mutation, the basic fold of the WT protein is preserved, but the deletion of the hydroxyl group in the aromatic side chain on substitution of phenylalanine for tyrosine in ... transfer step. In addition, the pK a of the remaining oxo bridge and the redox potentials of the metal centers may be altered in the site containing a...

Ngày tải lên: 08/03/2014, 08:20

... molecular tools for the inhibition of the biosynthesis of b-secretase and for the exclusion of the formation of toxic b-amyloid peptides in HEK293 cells. Materials and methods Construction of plasmids for ... asked the question whether we can inhibit expres- sion of the gene of BACE protein using our engineered ribozymes. In order to check the potential...

Ngày tải lên: 08/03/2014, 08:20