... Htelo
(Fig. S6). These findings indicate the importance of the
guanidinium group of the Arg in RGG3 for binding to
the G-quadruplex.
In conclusion, EWS appears to be a DNA- and
RNA-binding protein that ... DNA-binding motif in nucleolin [21–
27]. The RGG domain of FMRP, which is an RNA-
binding protein involved in nerve cell differentiation,
interacts with the G-quart...
... protein for an extrane-
ous ligand.
We cannot state that the natural ligand of the
H. pylori protein is erucamide, but the shape and size
of the cavity clearly indicate that inside the protein
there ... starting from the model of
the polyisoprenoid-binding protein from T. thermophilus
(Protein Data Bank ID: 1WUB [18]). Refinement was con-
tinued using the simulated...
... on the intensity of EGFP fluorescence
from the vector containing mutations in the region
encoding the miR-373 binding sites. These results
show that miR-373 binds directly to the 3¢-UTR of
the ... on the cell cycle (Fig. 6E).
Knockdown of PPP6C resulted in a significant
decrease in the proportion of cells in G
1
-phase and an
increase in the proportion of cells in...
... H
2
O
2
-induced apoptosis in transfected
C2C12 cells. Furthermore, there was no direct interac-
tion between HSP70 and Smac proteins, and the
ATP-binding domain of HSP70, rather than the pep-
tide-binding ... indicating that interaction with Smac is not
required with respect to the role of HSP70 in the inhi-
bition of the release of Smac and apoptosis.
The role of the...
... staining indicated that AtHELPS is mainly expressed in the young
seedlings and vascular tissues of leaves and roots. Using both helps mutants
and overexpression lines, we observed that, in the ... calci-
neurin B-like proteins, CBL1 and CBL9, were then
identified as calcium sensors in the differential regula-
tion of abiotic stress responses, and in the ABA sig-
nal...
... that has the IMPACT tag fused to the authentic
C-terminal Gly145.
Expression and purification
Recombinant protein was produced by cultivating the
expression strain in shaking flasks containing LB ... ligand binding, and the role of
storage proteins, the cupins constitute one of the most
diverse superfamilies of proteins known. They have
been described from all three domains...
... detectable in the cytoplasm and
nucleus (Fig. 2B). In FITC-labelled hpST3dODN-
transfected cells, phospho-STAT3 was detected in the
cytoplasm, but not in the nucleus, and ODN was
detected only in the cytoplasm ... (particularly in whole ani-
mals) by impairing the action of STAT1-dependent
interferon.
The results obtained in the present study, including...
... for the study of protein protein [22] and protein
nucleotide recognition [10,23,24], protein folding and
stability [25–28], protein dynamics [29], and cytotoxicity
[30]. The mechanism of the ... a guanine as the leading base, were docked into
the active site of the enzyme. In the five best-docked
dinucleotides in each protein molecule, the position of
the...
... found
to bind tightly to LPL in the vicinity of the active site
and also to inhibit the enzymatic activity [12]. Simi-
larly, we show here that bis-ANS inhibits the lipase
activity of HSL. Interestingly, ... phos-
phorylation. This gain in hydrophobic surface area
presumably accounts for the increase in in vitro activity
of HSL following PKA phosphorylation through
inc...
... than the
activity in control joints. The ubiquitinated protein lev-
els in synovial fluid from osteoarthritic joints were
higher than in the control joint group (Fig. 2A). We
also examined TRAIL protein ... joints was increased com-
pared with that in control joints. Changes in TRAIL
and ubiquitin levels may be linked to the progression
of in ammation and may be de...