Báo cáo khoa học: Salt-inducible kinase-1 represses cAMP response element-binding protein activity both in the nucleus and in the cytoplasm pdf
Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... Htelo (Fig. S6). These findings indicate the importance of the guanidinium group of the Arg in RGG3 for binding to the G-quadruplex. In conclusion, EWS appears to be a DNA- and RNA-binding protein that ... DNA-binding motif in nucleolin [21– 27]. The RGG domain of FMRP, which is an RNA- binding protein involved in nerve cell differentiation, interacts with the G-quart...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Helicobacter pylori acidic stress response factor HP1286 is a YceI homolog with new binding specificity pdf
... protein for an extrane- ous ligand. We cannot state that the natural ligand of the H. pylori protein is erucamide, but the shape and size of the cavity clearly indicate that inside the protein there ... starting from the model of the polyisoprenoid-binding protein from T. thermophilus (Protein Data Bank ID: 1WUB [18]). Refinement was con- tinued using the simulated...
Ngày tải lên: 16/02/2014, 14:20
Báo cáo khoa học: MicroRNA-373, a new regulator of protein phosphatase 6, functions as an oncogene in hepatocellular carcinoma pdf
... on the intensity of EGFP fluorescence from the vector containing mutations in the region encoding the miR-373 binding sites. These results show that miR-373 binds directly to the 3¢-UTR of the ... on the cell cycle (Fig. 6E). Knockdown of PPP6C resulted in a significant decrease in the proportion of cells in G 1 -phase and an increase in the proportion of cells in...
Ngày tải lên: 14/03/2014, 23:20
Báo cáo khoa học: ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells potx
... H 2 O 2 -induced apoptosis in transfected C2C12 cells. Furthermore, there was no direct interac- tion between HSP70 and Smac proteins, and the ATP-binding domain of HSP70, rather than the pep- tide-binding ... indicating that interaction with Smac is not required with respect to the role of HSP70 in the inhi- bition of the release of Smac and apoptosis. The role of the...
Ngày tải lên: 16/03/2014, 01:20
Tài liệu Báo cáo khoa học: A DExD⁄ H box RNA helicase is important for K+ deprivation responses and tolerance in Arabidopsis thaliana docx
... staining indicated that AtHELPS is mainly expressed in the young seedlings and vascular tissues of leaves and roots. Using both helps mutants and overexpression lines, we observed that, in the ... calci- neurin B-like proteins, CBL1 and CBL9, were then identified as calcium sensors in the differential regula- tion of abiotic stress responses, and in the ABA sig- nal...
Ngày tải lên: 14/02/2014, 18:20
Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... that has the IMPACT tag fused to the authentic C-terminal Gly145. Expression and purification Recombinant protein was produced by cultivating the expression strain in shaking flasks containing LB ... ligand binding, and the role of storage proteins, the cupins constitute one of the most diverse superfamilies of proteins known. They have been described from all three domains...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: Efficient killing of SW480 colon carcinoma cells by a signal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide – interference with interferon-c-STAT1-mediated killing pdf
... detectable in the cytoplasm and nucleus (Fig. 2B). In FITC-labelled hpST3dODN- transfected cells, phospho-STAT3 was detected in the cytoplasm, but not in the nucleus, and ODN was detected only in the cytoplasm ... (particularly in whole ani- mals) by impairing the action of STAT1-dependent interferon. The results obtained in the present study, including...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Structure of RNase Sa2 complexes with mononucleotides – new aspects of catalytic reaction and substrate recognition pptx
... for the study of protein protein [22] and protein nucleotide recognition [10,23,24], protein folding and stability [25–28], protein dynamics [29], and cytotoxicity [30]. The mechanism of the ... a guanine as the leading base, were docked into the active site of the enzyme. In the five best-docked dinucleotides in each protein molecule, the position of the...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt
... found to bind tightly to LPL in the vicinity of the active site and also to inhibit the enzymatic activity [12]. Simi- larly, we show here that bis-ANS inhibits the lipase activity of HSL. Interestingly, ... phos- phorylation. This gain in hydrophobic surface area presumably accounts for the increase in in vitro activity of HSL following PKA phosphorylation through inc...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Hypoxic resistance to articular chondrocyte apoptosis – a possible mechanism of maintaining homeostasis of normal articular cartilage pdf
... than the activity in control joints. The ubiquitinated protein lev- els in synovial fluid from osteoarthritic joints were higher than in the control joint group (Fig. 2A). We also examined TRAIL protein ... joints was increased com- pared with that in control joints. Changes in TRAIL and ubiquitin levels may be linked to the progression of in ammation and may be de...
Ngày tải lên: 18/02/2014, 14:20