Báo cáo khoa học: Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli potx

... time is required. Instead of expressing the oraE and oraS genes separately, the DNA fragment containing both genes was cloned i nto the same expression vector under the control of the T7 promoter, ... Comparison of t he deduced amino acid sequence of oraE to those of known PLP- dependent aminomutases reveals the presence of a con- served PLP-binding site, a lysine...

Ngày tải lên: 30/03/2014, 15:20

... preparation showed only one band when stained by GelCode Blue Stain Reagent and SilverXpress Silver Staining Kit in SDS gel electrophoresis (Fig. 1). Isoelectric focusing in the 2D gel detected a single ... excision and N-terminal sequence analysis by Edman degradation. The N-terminal sequence was deter- mined in the Protein Analysis Center, Karolinska Institutet (Stockholm, S...

Ngày tải lên: 07/03/2014, 14:20

... methionine residues, as suggested by MS/MS. The MS/MS sequencing also confirms the absence of the N-terminal Met1 residue of the a-subunit, in agreement with the predicted cleavage of the Met1-Gly2 sequence ... of these peaks are exactly the s ame for the protein recovered from crystals. However, the molecular masses for these two subunits predicted from the a...

Ngày tải lên: 30/03/2014, 15:20

... positions, while residues of the secondary sites are indicated by dots. In the top line, asterisks highlight the P1 and P¢2 sites. Residues conserved in at least 50% of the aligned sequences ... determining their protease-specificity) are found in a short segment containing the second conserved cysteine, a secondary site contacting the target proteases includes residues...

Ngày tải lên: 31/03/2014, 01:20

... observed in cells treated with cisplatin [50]. Interestingly, expression of genes with stronger promoters was strongly inhibited, whereas some genes possessing weaker promoters were induced. It was ... pPGM4 were used as the sequence-specific competitors, and changes in p53(1– 363) binding to the 32 P-labeled 50-mer targets were fol- lowed. We first tested the effect of the pr...

Ngày tải lên: 19/02/2014, 05:20

... certain in the case of invertebrates and basal metazoans. Nevertheless, the amino acid sequence of the Sycon group I PLA 2 demonstrates that the ancestor of group I PLA 2 s possessed the same ... purified from the milking exudates of several U. crassicornis specimens. On the basis of the N-terminal sequence of the isolated protein, its cDNA was isolated, seq...

Ngày tải lên: 06/03/2014, 11:20

... is that, as we said earlier, hedging is expressed via key- words in natural language texts; and often several keywords are present in a single sentence. The seed set S spec contained either suggest ... of these were the kind of phrases that had no unigram components of themselves in the feature set, so these could be re- garded as meaningful standalone features. Examples...

Ngày tải lên: 31/03/2014, 00:20

... consideration in further investi- gations of the role of this cytokine as an LPS mediator. The present PEC culture also showed the advantage of using mixed-cell cultures for the investigation of ... 2003 confirmed that the anti-(IL-18) Ig used in the present study does not inhibit the antigenicity of IL-12 by ELISA and that the same results as shown in the p...

Ngày tải lên: 17/03/2014, 10:20

... state. However, there is no clear match of the observed mass peaks to the predicted propeptides expected to originate from cleavage of signal peptides. Instead, further processing has been observed, ... controlled culture conditions, different strains of this taxon displayed different MS fingerprints. Because of the ease and speed of the analysis, we investigated whether t...

Ngày tải lên: 19/02/2014, 02:20

... proteins and their kin. Representatives of the four categories of scaffold proteins and their relatives are presented. The length of the human (unless indicated otherwise) isoform is given, along ... reviewed in two of the other minireviews in this series [5,7]. Here we pro- vide a short overview of their most notable examples, to demonstrate that the distinction b...

Ngày tải lên: 06/03/2014, 22:21