Báo cáo khoa học: Identification of intracellular target proteins of the calcium-signaling protein S100A12 pdf

... measurement of the equilibrium binding constant K a , the enthalpy of complex forma- tion (DH) and the complex stoichiometry of a protein protein interaction without the need for modification of the proteins ... where the presence of McsA enhances the autophosphorylation of McsB several-fold. We studied the binding pattern and the thermodynamic aspects of t...

Ngày tải lên: 23/03/2014, 06:20

... (1997) The role of CCAAT/enhancer-binding protein a and a protein that binds to the activator -protein- 1 site in the regulation of liver- specific expression of the winter flounder antifreeze protein ... degeneration of the sequences upstream of the pseudogenes made it impossible to predict the locations of the first introns and exons of Y1, Y2andY3. An align...

Ngày tải lên: 31/03/2014, 07:20

... member of the Hsp70/H sp90 multichaperone complex. J. Biol. Chem. 279, 4221–4233. Ó FEBS 2004 S100A12 target proteins (Eur. J. Biochem. 271) 3775 Identification of intracellular target proteins of the ... aggregation. Interaction of C-terminal truncation mutants of S100A12 and the S100A12 target proteins To further determine the role of S100A12 C-ter...

Ngày tải lên: 30/03/2014, 15:20

... determine whether RGG3 of EWS modulates the formation or unwinding of Htelo G-quadruplex DNA. To determine whether the RGG3 binding affected the stability of the G-quadruplex structure of Htelo, ... using imagequant software. The data were plotted as u (1 fraction of free DNA) versus the protein concentration to determine the K d , which is equal to the protein a...

Ngày tải lên: 15/02/2014, 01:20

... whether these cytokines are produced by CD4 + CD25 + Foxp3 + T reg or whether they induce the production of these cytokines from another population of cells [20]. To date, our knowledge about the ... to be protein domains impor- tant in the functioning of these transcription factors. The gene organiza- tions of zebrafish t-bet and foxp3 were identical to those of the huma...

Ngày tải lên: 16/02/2014, 09:20

... predictions of the deduced proteins were performed using tmpred, toppred and pre- dictprotein [43–45]. Similarities of these two proteins with late acyltransferase homologs in several other Gram-nega- tive ... regions anchoring the proteins to the inner membrane but not in the cytoplasm of the bacterium (data not shown), which is consistent with those of defined E. coli...

Ngày tải lên: 18/02/2014, 14:20

... LacZ filter-lift assays. The development of a blue color through the activation of the yeast LacZ reporter gene is another indication of a protein protein interaction. For the yeast three-hybrid ... includes the homologous proteins Drosophila MAD and the vertebrate Smad1, Fig. 1. Structure of the SmSmad1B gene, cDNA and protein. (A) Schematic representations of th...

Ngày tải lên: 18/02/2014, 16:20

... trophosome-specific proteins. We demonstrated the existence of carbonic anhydrase transcripts, a protein endowed with an essential role in generating the influx of CO 2 required by the symbionts. One of the transcripts ... concluded that there were two CA proteins: one of 27 kDa in the branchial plume, and another of 28 kDa in the trophosome. From the differential ex...

Ngày tải lên: 18/02/2014, 16:20

... folding of cytoskeletal proteins that are actively synthesized and ⁄ or renatured under these conditions. The upregu- lation of all of these chaperones confirms the severity of the thermal stress under ... constitutive isoforms of HSP70 are synthesized in the gills and mantle of C. gigas [26]. The expression level of the constitutive forms increases after thermal st...

Ngày tải lên: 18/02/2014, 16:20

... confirmed to be otolin-1. Therefore, MALDI-TOF- TOF tandem mass spectrometry was performed to identify the proteins. The tryptic peptide mass finger- printing spectra of these proteins were highly ... elec- trophoresis. These substances may be aggregates of proteins and polysaccharides, and may play important roles in formation of the phases and ⁄ or morphologies of the cry...

Ngày tải lên: 18/02/2014, 17:20