Báo cáo khóa học: Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose ppt

... http://www.ncbi.nlm.nih. gov/UniGene). Our analysis of the expression of the enzymes involved in the salvage pathway of GDP- L -fucose indicates that not only the de novo pathway alone, but also the salvage pathway could have ... role in the synthesis of GDP- L -fucose in the cytosol. The importance and the regulatory mechanisms of the en...

Ngày tải lên: 30/03/2014, 13:20

... Li R, Hartley L & Robb L (2001) Cloning of rat inter- leukin 11 and interleukin 11 receptor alpha chain and analysis of their expression in rat uterus in the peri- implantation period. Reproduction ... macrophages [12]. In combination with its trophic effects on the gastrointestinal epithelium, IL-11 has a profound activity in the protection and restoration of...

Ngày tải lên: 07/03/2014, 16:20

... Sequencing and annealing of the 5¢ and the 3¢ sequence revealed an ORF of 789 bp, encoding a 262 amino acid protein (Fig. 1). All four p eptides from the purified tomato protein could be identified in the deduced ... cDNA and deduced protein sequence. Pe ptides determined by seq ue ncing of the p urified protein are boxed and the names of the peptides are indicate...

Ngày tải lên: 16/03/2014, 18:20

... (calnexin) and cytoskeleton protein (tubulin) in these cells as the internal control proteins. The calnexin and tubulin levels were found to be similar between these cells, confirming that the same amount ... results indicate that a functional deletion of Synoviolin causes a change in the intracellular trafficking of NCT. Effect of Synoviolin on the production of Ab NC...

Ngày tải lên: 23/03/2014, 04:20

... proteins in E. coli. It allows the expression of the recombinant protein and results in the addition of a short noncleavable His tag sequence at its N-terminus. Cloning resulted in incorporation of ... sites involved in activation and a third low-affinity site involved in inhibition [13]. In the crystal structure, two Mg 2+ -binding sites h ave been detect...

Ngày tải lên: 16/03/2014, 18:20

... calcium binding domains (EF1–4) and calcineurin A binding domain are shown in the box. The dot in the EF1 box repre- sents the modified amino acids alanine (A) as compared to the oxygen containing-calcium ... unshaded. The amino acids marked by red, blue, green and pink lines indicates the putative casein kinase II, protein kinase C, the cAMP- and cGMP-dependent prote...

Ngày tải lên: 19/02/2014, 07:20

... 271) 705 encoding the DNA-binding domain of HSF, whereas clone C2 contained an insert of 2771 nucleotides including introns and an ORF encoding 513 amino acids. By using 5¢ -and3 ¢-RACE, the full-length ... (a 3 and b 3 ) and two heterotrimers (a 2 b 1 and a 1 b 2 ). The existence of the four types of trimer may be reflected in the broad band migrating at  200 kDa...

Ngày tải lên: 19/02/2014, 12:20

... (Met153 of the ML1p and ML2p B-chains and Met156 of the ML3p B-chain corresponding to the ricin B-chain Leu152; and Met234 of the ML1p and ML2p B-chains corresponding to the ricin B-chain Leu233). Estimation ... Asn255 and Gln256 of the ricin B-chain in the second sugar binding site [28]. The exceptions are the replacement of Trp38 fi Ser in the ML2p...

Ngày tải lên: 07/03/2014, 15:20

... bovine D14-SR, regions of the deduced amino-acid sequence corresponding to the N-terminal and V8 peptide sequences determined in the sequencing experiments of the protein puri®ed from b ovine ... our hypothesis, bovine cDNA encoding the 38-kDa protein was cloned to iden tify the catalytic activity of the expressed protein. Cloning of the cDNA encoding bovine ster...

Ngày tải lên: 08/03/2014, 16:20

... Scot- land. Minqing Hu and Bing Liu. 2004. Mining and sum- marizing customer reviews. In KDD’04: Proceed- ings of the Tenth ACM SIGKDD International Con- ference on Knowledge Discovery and Data Mining, pages ... marking an opin- ion expression span, the target and holder of the opinion is marked and linked to the marked opin- ion expression. Furthermore, the expr...

Ngày tải lên: 16/03/2014, 23:20