Báo cáo khóa học: Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose ppt

... http://www.ncbi.nlm.nih.gov/UniGene). Our analysis of the expression of the enzymes involved in the salvage pathway of GDP-L-fucose indicatesthat not only the de novo pathway alone, but also the salvage pathway could have ... role in the synthesis of GDP-L-fucose in the cytosol. The importance and the regulatory mechanisms of the enzymes in the salvage pathway of GDP-L-fucose have not been elucidated, thusfuther ... Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucoseL-fucokinase and GDP-L-fucose pyrophosphorylaseJaana...

Báo cáo khoa học: Cloning and expression of the ﬁrst nonmammalian interleukin-11 gene in rainbow trout Oncorhynchus mykiss pdf

... Li R, Hartley L & Robb L (2001) Cloning of rat inter-leukin 11 and interleukin 11 receptor alpha chain and analysis of their expression in rat uterus in the peri-implantation period. Reproduction ... macrophages[12]. In combination with its trophic effects on the gastrointestinal epithelium, IL-11 has a profoundactivity in the protection and restoration of the gastrointestinal mucosa [13,14]. Interestingly ... from other species and separated from other members of the IL-6 family. The IL-11 gene is highlyexpressed in intestine and gills in healthy ﬁsh and its expression can also bedetected in spleen,...

Báo cáo khoa học: Cloning and expression of a tomato cDNA encoding a methyl jasmonate cleaving esterase pdf

... Sequencing and annealing of the 5¢ and the 3¢ sequence revealed an ORF of 789 bp,encoding a 262 amino acid protein (Fig. 1). All four p eptidesfrom the puriﬁed tomato protein could be identiﬁed in the deduced ... cDNA and deduced proteinsequence. Pe ptides determined by seq ue ncing of the p uriﬁed protein areboxed and the names of the peptides are indicated in italic lettersabove. Nucleic acids o f the ... Software (Informax). Thereby, amodiﬁed N-terminus of the encoded protein was created in which Glu and Lys in positions 2 and 3, respectively, werereplaced with a single Gln.At the C -terminus...

Báo cáo khoa học: An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid b-protein doc

... (calnexin) and cytoskeletonprotein (tubulin) in these cells as the internal controlproteins. The calnexin and tubulin levels were found tobe similar between these cells, conﬁrming that the sameamount ... results indicatethat a functional deletion of Synoviolin causes a change in the intracellular trafﬁcking of NCT.Effect of Synoviolin on the production of AbNCT is one of the essential cofactors of ... Synoviolin is involved in the degradation of immature NCT. Wehave also shown that the expression of Synoviolinenhances Ab generation. Further study of the mechan-ism underlying the enhancement of...

Báo cáo khoa học: Cloning, over-expression, puriﬁcation and characterization of Plasmodium falciparum enolase doc

... proteins in E. coli. It allows the expression of the recombinant protein and results in the addition of a short noncleavable His tag sequence at itsN-terminus. Cloning resulted in incorporation of ... sites involved in activation and a third low-afﬁnity site involved in inhibition [13]. In the crystal structure, twoMg2+-binding sites h ave been detected. These are believedto be involved in ... remains one of the most infectious diseases in the third world with abou t 500 million infections and over onemillion deaths per year [1]. In the face of increasing threatsby resurgent infections...

Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

... calcium binding domains (EF1–4) and calcineurin A binding domain are shown in the box. The dot in the EF1 box repre-sents the modiﬁed amino acids alanine (A) as compared to the oxygen containing-calcium ... unshaded. The amino acids marked by red, blue, green and pink lines indicates the putative casein kinase II, protein kinase C, the cAMP- and cGMP-dependent protein kinase and putative tyrosinekinase ... deletion in the autoinhibitory(NAF) motif failed to interact with PsCBL thus con-ﬁrming the authenticity of these proteins and emphasi-zing the importance NAF in the interaction betweenthem (data...

Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt

... 271) 705encoding the DNA-binding domain of HSF, whereas cloneC2 contained an insert of 2771 nucleotides including introns and an ORF encoding 513 amino acids.By using 5¢ -and3 ¢-RACE, the full-length ... (a3 and b3) and two heterotrimers (a2b1 and a1b2). The existence of the four types of trimer may be reﬂected in the broadband migrating at  200 kDa in Fig. 7. On the other hand,a band ... cycle of 15 min at 95 °C,ampliﬁcation cycles of 0.5 min at 94 °C, 0.5 min at 50 °C and 0.5 min at 72 °C, and one termination cycle of 1 min at72 °C, with 35 cycles in total for HSF1a and HSF1b and...

Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

... (Met153 of the ML1p and ML2p B-chains and Met156 of the ML3p B-chain corresponding to the ricinB-chain Leu152; and Met234 of the ML1p and ML2pB-chains corresponding to the ricin B-chain Leu233).Estimation ... Asn255 and Gln256 of the ricin B-chain in the second sugar binding site [28]. The exceptions are the replacement of Trp38 ﬁ Ser in the ML2p B-chain and Tyr252 ﬁ Phe in the ML3p B-chain (Fig. 2). The ... However, the amino acidsforming the catalytically active center of the A-chains of the MLp toxins are fully conserved and retained in corresponding positions, as in the A-chain of ricin [27] and MLI...

Báo cáo Y học: Cloning and expression of sterol D14-reductase from bovine liver potx

... bovineD14-SR, regions of the deduced amino-acid sequence corresponding to the N-terminal and V8 peptide sequences determined in the sequencingexperiments of the protein puri®ed from b ovine ... our hypothesis, bovine cDNA encoding the 38-kDa protein was cloned to iden tify the catalyticactivity of the expressed protein. Cloning of the cDNA encoding bovine sterolD14-reductaseBovine cDNA ... encoding a protein of 418 amino a cids with acalculated molecular mass of 46 751 Da. The N-terminal amino-acid sequence of the proteinpuri®ed from liver and the amino-acid sequence of the 19.5-kDa...

Báo cáo khoa học: "Sentence and Expression Level Annotation of Opinions in User-Generated Discourse" potx

... Scot-land.Minqing Hu and Bing Liu. 2004. Mining and sum-marizing customer reviews. In KDD’04: Proceed-ings of the Tenth ACM SIGKDD International Con-ference on Knowledge Discovery and Data Mining,pages ... marking an opin-ion expression span, the target and holder of the opinion is marked and linked to the marked opin-ion expression. Furthermore, the expression levelscheme allows assigning polarities ... containing eval-uations about the topic are further investi-gated at the expression level for pinpoint-ing the properties (semantic orientation,intensity), and the functional components of the...

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