Báo cáo khoa học: Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L docx

... (2001) Molecular cloning and characterization of cDNA encoding cardosin B, an aspartic proteinase accumulating extracellularly in the transmitting tissue of Cynara cardunculus L. Plant Mol Biol 45, ... 2523–2539 ª 2007 The Authors Journal compilation ª 2007 FEBS 2539 Characterization and expression analysis of the aspartic protease gene family of Cyna...

Ngày tải lên: 30/03/2014, 09:20

... above. Construction of full-length xTRHR cDNAs. We used the following strategy as we were unable to amplify the full- length cDNA directly by nested PCR, probably due to the too low expression and the large ... especi- ally xTRHR3 and xTRHR2. This is particularly interesting considering the functional importance of the third intracel- lular loop and the C-terminal ta...

Ngày tải lên: 21/02/2014, 03:20

... structures of EcR and USP from L. decemlineata (b) the analysis of mRNA expression profile of L. decemlineata, EcR and USP, and (c) the measurement of the binding affinity of steroidal and nonsteroidal ... expres- sion level until day 8. Total RNAs from the whole body of male and female adult, testis and ovaries as well as from L. decemlineata cells were s...

Ngày tải lên: 07/03/2014, 21:20

... University, the Netherlands Pectin is a complex polysaccharide present in the cell wall of higher plants, where it forms a network by embedding the other cell wall polysaccharides cellulose and hemicellulose. ... 28 of the glycoside hydrolases, including the endopolygalacturonases, exo- polygalacturonases and rhamnogalacturonases [3,4]. Although a handful of endopolygalact...

Ngày tải lên: 20/02/2014, 03:20

... from the fol- lowing set {DOMAIN, ADJ, UNIQUE}) are added to the Lemma and Stem+Morph settings. With all 588 Word POS Surface form Lemma Stem Gloss AlwlAyAt Noun Al+wlAyAt Al+wlAyp Al+wlAy the ... systems have been built, e.g., (Yi et al. 2003; Hu and Liu., 2004; Kim and Hovy., 2004; Mullen and Collier 2004; Pang and Lee 2004; Wilson et al. 2005; Yu and Hatzivassiloglou, 200...

Ngày tải lên: 20/02/2014, 05:20

... cancer cell lines, where 14-3-3f and 14-3-3h were expressed well and uni- formly. Meanwhile, other human 14-3-3 isoforms showed low expression levels in HeLa cells, and were also differently expressed ... expanded. FACS analysis was also performed to ensure uniform expression of GFP in the selected cell lines, and anti-14-3-3f immunoblotting analysis of lysed cells was p...

Ngày tải lên: 06/03/2014, 22:21

... simplicity, and in line with the results from the other calculations, the <0.0 value is allocated to Asp140 and > 20.0 to Asp142. b The calculations were performed on the crystal structure of the ... second shell, as well as Asp140, Asp142, Glu144 and Asp215 were included fully in the calculation, whereas all other groups were treated less rigorously. This approach...

Ngày tải lên: 07/03/2014, 14:20

... of Pex1p and to D1 and D2 of Pex6p as well as the capability for ATP-hydrolysis in general is dispensible for the Pex1p–Pex6p interaction. However, the results also clearly demonstrate that the ... characterized by the inability to grow on oleic acid as the single car- bon source, mislocalization of matrix proteins to the cytosol and the absence of morphologicall...

Ngày tải lên: 07/03/2014, 16:20

... Phylogenetic analysis clearly grouped trout IL-11 with IL-11 molecules from other species and separated from other members of the IL-6 family. The IL-11 gene is highly expressed in intestine and ... head kidney and spleen. IL-11 expression is also up-regulated in RTS-11 cells (a trout macrophage cell line), which constitutively expressed the lowest level of IL-11 of...

Ngày tải lên: 07/03/2014, 16:20

... at the wandering stage late in the last larval stadium. At the prepupal stage, EH activity declined to a level equal to that in the early time of the stadium. Northern blot analysis revealed ... and the cellular proteins were analysed in a 12% (w/v) gel. A distinct band of 43 kDa was observed in all four samples, and no band of this size was seen in the control sampl...

Ngày tải lên: 07/03/2014, 21:20