Báo cáo khoa học: Nop53p interacts with 5 8S rRNA co-transcriptionally, and regulates processing of pre-rRNA by the exosome ppt

... processing and to co-immunoprecipitate the 27S and 7S pre-rRNAs and the mature 5. 8S rRNA [31–33], and to bind 5. 8S rRNA in vitro [31]. In order to determine whether Nop53p interacts with the pre -rRNA ... degraded by the exosome in the absence of Nop53p. In order to determine whether the effect of Nop53p depletion on 5. 8S processing...

Ngày tải lên: 30/03/2014, 04:20

... inves- tigate whether interaction with CD91 takes place at or near the MASP-binding site, the ability of the MBL mutants K55A and K55Q, which lack the abil- ity to associate with MASPs, to interact with ... concentration of active CD91 and there- fore the kinetics of the interaction when CD91 is in solution. The K D value obtained was 9.33 nm with a v 2 = 0.899...

Ngày tải lên: 06/03/2014, 01:23

... conserved, with 87% sequence similarity with the m urine homologue. The murine Nbr1 gene liesheadtoheadwiththeBrca1 gene, with the intragenic region of 289 bp suggesting the possibility of co-ordinated expression ... at 55 °C. The s lides were then washed at high stringency (20 min at 55 °Cin2´ NaCl/Cit, 50 % formamide,10 m M dithiothreitol) and then treated with 40...

Ngày tải lên: 08/03/2014, 10:20

... mechanism underlying the regulation of this pathway by Nucling remains to be elucidated. The classic form of NF- jB is the heterodimer of the p50 and p 65 subunits, which is sequestered in the cyto- plasm ... overexpressed by cotrans- fection (Fig. 5B,C,F,G). However, when NuclingDN was coexpressed with NF-jB (p50 or p 65) , over 65% of cells showed nuclear staining...

Ngày tải lên: 16/03/2014, 04:20

... 2007) doi:10.1111/j.1742-4 658 .2007. 059 28.x The EVI1 transcriptional repressor is critical to the normal development of a variety of tissues and participates in the progression of acute myeloid leu- kaemias. The repressor ... that the gene nor- mally encodes a putative protein of 157 amino acids with a predicted molecular mass of 18.2 kDa. Interro- gation of the...

Ngày tải lên: 16/03/2014, 11:20

... structure of the Dbl and pleckstrin homology domains from the human Son of sevenless protein. Cell 95, 259 ±268. 36. Hall, A. (1998) Rho G TPases and the actin c ytoskeleton. Science 279, 50 9 51 4. 37. ... triggered by t he Gbc 25. Moreover, the co-expression of Ras-GRF1 and G bc subunits leads to the activation of the MAP kinases JNK1 and ERK2 in heterologous...

Ngày tải lên: 17/03/2014, 11:20

... that the pres- ence of the two terminal short stretches of adenines at the 5 and the 3¢ ends of the ARS were not essential for binding to IMP1 (Fig. 3B: lanes 2 and 3, and the sequence of ARS and ... structure of 5. 8S rRNA from a thermo- phile, Thermomyces lanuginosus. J Biol Chem 256 , 56 75 56 82. 38 Caputi M & Zahler AM (2001) Determination of t...

Ngày tải lên: 23/03/2014, 10:20

... acti- vated by many stimulants, and regulates the level of IL-1b mRNA and of its own transcript. Further stud- ies are necessary to clarify the biological role of MCPIP as a nuclease and to identify the ... Structures of the PIN domains of SMG6 and SMG5 reveal a nuclease within the mRNA surveillance complex. EMBO J 25, 51 17 51 25. 8 Thompson JD, Gibson TJ, P...

Ngày tải lên: 18/02/2014, 14:20

... the set of case elements that modify the i-th verb in T rs i (T ) : the set of particles in the set of case el- ements that modify the i-th verb in T ns i (T ) : the set of nouns in the set of ... )) the co-occurrence prob- ability of the particle set and the verb, and we call P(n i,j (T )|r i,j (T ), v i (T )) the co-occurrence probability of the cas...

Ngày tải lên: 20/02/2014, 12:20

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Ngày tải lên: 06/03/2014, 22:21