Báo cáo khoa học: The phosphate site of trehalose phosphorylase from Schizophyllum communeprobed by site-directed mutagenesis and chemical rescue studies pptx

... of trehalose phosphorylase from Schizophyllum commune probed by site- directed mutagenesis and chemical rescue studies Christiane Goedl and Bernd Nidetzky Institute of Biotechnology and Biochemical ... feature of the mechanism is the devel- opment of a strong hydrogen bond between the incoming nucleophile and the leaving group in the transition sta...

Ngày tải lên: 30/03/2014, 04:20

... shown in the figure. (C) Time course of the degradation of (GlcNAc) 3 (r) and (GlcNAc) 4 ( )byLlChi18A, illustrated by the production of (GlcNAc) 2 during the initial linear phase of the degradation ... example of the role of family 33 CBPs in the deg- radation of recalcitrant polysaccharides. Furthermore, the results provide the first example of a family 3...

Ngày tải lên: 18/02/2014, 08:20

... hat binding sites i n domains II, III and IV are either distorted or the access of a cation to the site is blocked by the p resence of a side chain of a b asic residue. I n case of the IIAB s ... 2. The membrane binding loops. The IAB loops of Anx(Gh1) (A) and AnxA5 (B) are shown in the same view from the front. (C) and (D) show the IIAB loops of Anx(...

Ngày tải lên: 17/03/2014, 03:20

... thiamine diphosphate (ThDP) is the deprotonation of the C2 atom of the thiazolium ring (marked by an asterisk). The resulting ylid of ThDP (I) can attack the carbon atom of the carbonyl group of the ... 4203 Structure of the active site The general architecture of the active site of KlPDC corresponds to that of other ThDP-dependent enzymes. Figures...

Ngày tải lên: 30/03/2014, 10:20

... respiration and photosynthesis). Analysis of the amino acid sequence of the cloned catalytic PHO a-subunit further emphasize the crucial role of hydrophobic interactions occurring in the active site ... was isolated by our group [27]. This paper describes the purification, the characterization and the catalytic properties of the oxygenase component of PH from...

Ngày tải lên: 31/03/2014, 01:20

... measuring the mRNA levels and measuring the activity of the promoters of the two genes in response to TGFb stimulation. First, we determined the effect of TGFb on the mRNA levels of the RhoA and RhoB ... normalization of the samples, the cDNA of the housekeeping glyceraldehyde-3 -phosphate dehydro- genase (GAPDH) gene was also amplified by PCR. Quantifi- c...

Ngày tải lên: 16/03/2014, 06:20

... experiments, the position of the binding site for ATP at the front side of the N-domain between I390 and L576 and reveals the separate existence of a p-nitrophenylphosphatase at the rear site o f the N-domain. ... bond of the NO 2 -group of pNPP with the amino group of N398. The hydrophobicity of the binding pocket is achieved by W411 and L414....

Ngày tải lên: 19/02/2014, 16:20

... and Y338 of ClfA. One of the objectives of this study was to determine the mechanism of Fg binding by the A domain of FnBPB. Located distal to the A domains of FnBPA and FnBPB are multiple tandemly ... a 5 b 1 [3]. The subsequent internalization of S. aureus protects the bacterium from the host immune system and promotes its spread from the site of...

Ngày tải lên: 06/03/2014, 00:20

... normal starter acyl unit is clearly regulated by the rate of release of the diketide product by the TE domain. Studies of the extent of loading of multienzymes with more than one module are needed to ... combina- tions of substrates that should allow synthesis of a diketide product and then analyzed as before to deter- mine the nature and extent of occupanc...

Ngày tải lên: 16/03/2014, 03:20

... Recently, residues in the putative distal active site of KatGs have been the targets of site- directed mutagenesis studies. The role of distal Trp, Arg and His was studied in the KatGs from Escherichia ... inspection of the crystal structures of members of the plant, fungal and bacterial superfamily show the existence of a hydrogen bond between the d...

Ngày tải lên: 23/03/2014, 21:20