Báo cáo khoa học: Functional reconstitution of mammalian ‘chloride intracellular channels’ CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin docx

... 2007 FEBS Functional reconstitution of mammalian ‘chloride intracellular channels’ CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin H. Singh,* M. A. Cousin and R. H. ... CLIC5. CLIC5 also associates with cytoskeletal filaments [16], but the functional conse- quences of such interactions are unknown. We set out to expres...

Ngày tải lên: 30/03/2014, 04:20

... 587 MINIREVIEW The reconstitution of mammalian prion infectivity de novo Ilia V. Baskakov Medical Biotechnology Center, University of Maryland Biotechnology Institute, and Department of Biochemistry and Molecular ... prion biology of yeast and fungus supports this idea strongly, the direct proof of the prion hypothesis in mammals, specifically the reconstitution of the...

Ngày tải lên: 30/03/2014, 09:20

... suppressive activity of DEC1. Determination of the binding domain of DEC1 to BMAL1 The interaction of DEC1 and BMAL1 was previously demonstrated by a yeast two-hybrid assay [ 7], and this interaction ... (lanes 8 and 17). Shifted bands of radiolabelled E-box C a nd m utant DEC1 complexes are indicated by asterisks. Expres- sion levels of DEC1 mutants and VP16-DEC1 muta...

Ngày tải lên: 19/02/2014, 16:20

... number of mutant L183P is lower than that of wild-type GALE by a factor of three [22], the relative abundance of this mutant protein was greatly reduced in bacteria [22] and yeast [23], thereby decreasing ... misfolded proteins and con- tain components of the protein degradation and refold- ing machinery, including proteasome complexes and members of the Hsc70 and Hsp7...

Ngày tải lên: 07/03/2014, 00:20

... K d values of 1.647 nm (confidence interval of )0.5963 to 3.891, R2 of 0.9292, degree of freedom of 10) and 1.447 nm (confidence interval of 0.9588–1.935, R2 of 0.9788, degree of freedom of 28), respectively. ... absence of ligand and are activated by subnanomolar concentrations of lig- and. One concern about this system for in vivo applica- tions is the use of RX...

Ngày tải lên: 16/03/2014, 12:20

... Wellenreuther and W. Meyer-Klaucke for data collection and assis- tance in data evaluation. The assistance of T. Pavkov (Institute of Chemistry, University of Graz) in the acquisition of CD and DLS ... (1971) The kinetics and mechanisms of the reactions of iron(III) with b-dik- etones. The formation of monoacetylacetoatoiron(III) and the effect of copper (II) on the...

Ngày tải lên: 18/02/2014, 06:20

... complex of E. coli is com- posed of one copy each of membrane subunits LolC and LolE, and two copies of ATPase subunit LolD [7]. Both LolC and LolE are assumed to span the mem- brane four times and ... rather simple method; incubation of subunits at 30 °C in the presence of phospholipids leads to the reconstitution of the functional LolCDE complex (Figs 3 and 4). O...

Ngày tải lên: 19/02/2014, 00:20

... Kirschbaum B & Loffler M (1996) Functional expression of a fragment of human dihydroorotate dehydrogenase by means of the baculovirus expression vector system, and kinetic inves- tigation of the purified ... mechanism and binding of substrates was investi- gated for the multisubstrate Drosophila melanogaster deoxyribonucleoside kinase. Mutation of E52 to D, Q and H plu...

Ngày tải lên: 19/02/2014, 02:20

... proteins, and the reaction was started by adding 50 lL per well of plasminogen and of CBS0065 in the same buffer. Kinetics of plasmin formation was followed for 5 h by measuring the release of p-nitroaniline, ... Glu-Pg- and mAb A10.2-treated cells; lane 6, Glu-Pg- and mAb 34D3-trea- ted cells. Bands in lanes 1 and 2 correspond to forms I and II of plasmin(ogen). Hig...

Ngày tải lên: 20/02/2014, 01:20

... between helices 3 and 4 at the junction crossover – and the unpairing of four base-pairs in the centre of the junctions [19]. Detailed understanding of the functional role of these interactions would ... strains, plasmids and expertise in the course of these experiments, A. Pittman for help with the cloning of the mutants, M. Whitby (University of Oxford, UK) and I. Ha...

Ngày tải lên: 20/02/2014, 11:20