Báo cáo khoa học: Molecular cloning of the ecdysone receptor and the retinoid X receptor from the scorpion Liocheles australasiae pot

... into two exons (IA and IB) in the X. laevis gene, with the site of the intron insertion localized within the 5¢ UTR region of t he xMGP gene (Fig. 1 and Table 2 ). The other introns (2, 3 and 4) ... [23]. The construction of the )70/)36TATA LUC and 3x( )70/)36)TATALUC involved t he cloning of one or three copies of double stranded oligonucleotides spanning...

Ngày tải lên: 08/03/2014, 10:20

... sequence of the L. australasiae ecdysone receptor was similar to that of the ecdysone receptor- A of the soft tick, Ornithodoros moubata (68%) and to that of the ecdysone receptor- A1 of the lone ... the ecdysone receptor and the retinoid X receptor were cloned from the Japanese scorpion Liocheles australasiae, and the amino acid...

Ngày tải lên: 30/03/2014, 04:20

... and one oxygen atom of the side chain carboxylic group of Glu668 and between the carbonyl carbon atom of Pro of the substrate Ala-Pro-pNA and the Ser630 oxygen atom of the enzyme to hold the substrate ... affect the type of inhibition and the K i value of the oligopeptides Tat(1–9) and Trp2-Tat(1–9). On the other hand, corres- ponding to the fourf...

Ngày tải lên: 20/02/2014, 11:20

... receptor of the ligand, whereas the main core structure binds into the epitope for the type II receptor of the BMP ligand. Therefore, the binding of the receptors of both subtypes is blocked and BMP ... the membrane. The binding domains of the receptor chains have no contact with each other. They are bound together solely by the BMP ligand. The BMP re...

Ngày tải lên: 15/03/2014, 09:20

... that the major T-cell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight, 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and the ... constant (K d ) of 4.8 · 10 )7 M. The thermal unfolding of the heterodimer was completely reversible, with a T m of 48 °C. The comparative thermodynamics and therm...

Ngày tải lên: 22/03/2014, 16:21

... A ˚ ) to the carbonyl oxygen of Asp25¢, while the other conformation had the Ce atom at 5.51 A ˚ from the carbonyl oxygen of Asp25¢. In comparison, the Leu90 and Leu90¢ in the PR showed the closest ... atom of indinavir formed hydrogen bond interactions with the amide and OD2 of Asp29¢ ,and interacted via water OB with the carbonyl oxygen of Gly27, the OD1...

Ngày tải lên: 23/03/2014, 12:20

... structures of EcR and USP from L. decemlineata (b) the analysis of mRNA expression profile of L. decemlineata, EcR and USP, and (c) the measurement of the binding affinity of steroidal and nonsteroidal ecdysone ... dipteran, the yellow fever mosquitoe Aedes aegypti [34], and lepidopterans, the tobacco hornworm Manduca sexta [35–37] and the silkworm Bombyx m...

Ngày tải lên: 07/03/2014, 21:20

... fitted to: G ¼ G max / (1 + exp[–zÆ(V-V ½ )/25]), where G max is the maximum conductance, z is the valence of the process and V ½ is the midpoint voltage of activation. Ionic current expression levels ... SCN1B .The SCN1B gene spans  9kbonchromosome19 across six exons. Exon 3A is an extended exon 3(retentionofpartofintron3)viaalternative splicing. Exons 1, 2, 3, 4 and 5 (soli...

Ngày tải lên: 16/03/2014, 23:20

... possessing six conserved cysteine residues at the same positions as that of other CHHs of penaeid shrimps (Fig. 1B). Tissue-specific gene expression of L. schmitti CHH gene The size and expression of the ... osmoregulation [10,11] and lipid metabolism [12] in different species. Although the X- organ–sinus gland complex is con- sidered the main source of CHH production,...

Ngày tải lên: 23/03/2014, 10:20

... (1982) Actions of insect toxins and other toxins derivated from the venom of the Scorpion Androctonus australis on isolated giant axons of the cockroach (Periplameta americana). J. Exp. Biol. 97, ... similar to that of other scorpion sodium toxins, but so far it is the longest among known scorpion toxin introns. Construction of expression vectors and expression...

Ngày tải lên: 31/03/2014, 09:20