Báo cáo khoa học: Altered expression of tumor protein D52 regulates apoptosis and migration of prostate cancer cells potx
Báo cáo khoa học: Altered expression of tumor protein D52 regulates apoptosis and migration of prostate cancer cells potx
... The Authors Journal compilation ª 2008 FEBS 5713 Altered expression of tumor protein D52 regulates apoptosis and migration of prostate cancer cells Ramesh Ummanni1, Steffen Teller1, Heike ... define the protein signature of pros-tate cancer biopsies revealed the overexpression of TPD52 in cancer patient material [22]. To date, themain physiological role of TPD52 in prostate cancer progression ... thatdysregulation of TPD52 expression slightly altered theproliferation of LNCaP cells. A common molecular strategy used by tumor cells to evade apoptosis is the upregulation of anti-apopto-tic proteins...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... Iron loading of HepG2 cells resulted in increased expression of Nor3.2-reactive CD1d molecules at the plasma membrane. Expression of classical MHC class I and II molecules, ICAM-1 and the epithelial ... loading of the liver epithelial cell line HepG2 influenced the expression of immune regulatory molecules known tofunction as ligands of selected subsets of T cells, suchas MHC class I and II, ... after 1 week of culture ( 20% of cells positive for iron) and reached a plateau after8 weeks of culture ( 60% of cells positive, Fig. 2A).In all subsequent experiments, HepG2 cells were cul-tured...
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Tài liệu Báo cáo khoa học: Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins pdf
... Group in Protein Structure and Function, Department of Biochemistry and Protein Engineering Network of Centres of Excellence, University of Alberta, Edmonton, Alberta, CanadaAntifreeze proteins ... of the coils and the helicalhandedness are d ifferent, w ith t he s pruce budworm protein consisting of 15-residue coils with a left-handed fold and theTenebrio molitor protein consisting of ... comparison of s bwAFP and TmAFPwitheachotherandwithproteinsthathaveasimilar fold.Structure of sbwAFP and TmAFPThe structure of sbwAFP has been determined by X-raycrystallography to 2.5 A˚ and by...
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Báo cáo khoa học: A role for serglycin proteoglycan in granular retention and processing of mast cell secretory granule components ppt
... species and subclass of MC [1,3]. Histamine is amajor constituent of all types of MC, and it is nowwell recognized that MC granules can contain a num-ber of different cytokines, such as tumor ... mMCP-6 and CPAtranscripts were detected in both SG+ ⁄ + and SG– ⁄ – cells, and the kinetics as regards onset of mRNA expression were similar in both genotypes, indicatingthat the knockout of ... a flow rate of 0.5 mLÆmin)1. Fractions (0.5 mL)were collected and analyzed for35S radioactivity. As aninternal standard, 200 lL of a mixture of unlabeled heparin(4 mgÆmL)1) and CS-A (5...
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Báo cáo khoa học: Light-harvesting complex II protein CP29 binds to photosystem I of Chlamydomonas reinhardtii under State 2 conditions doc
... reinhardtii cells. (A) Protein profiles of thylakoids (Thy) and LHCI–PSI (F3) complexes obtained from psbD-His State 1- (S1) and State 2 (S2)-induced cells. (B) Phosphory-lation of thylakoids and LHCI–PSI ... corresponding tothe LHCI–PSI supercomplexes (S1–F3 and S2–F3 frac-tions) [16]. The protein profiles of S1 and S2 thyla-koids (Thy) and also of the S1 ⁄ S2–F3 sucrose-gradientfactions (Fig. 2A) ... staining) and similar tothe S1 profiles reported previously [16]. Western blot-ting and spectroscopic analyses of S1–F3 and S2–F3fractions confirmed the presence of PSI core subunits and the functionally...
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Báo cáo khoa học: Human papillomavirus 16 E7 protein inhibits interferon-c-mediated enhancement of keratinocyte antigen processing and T-cell lysis docx
... levels of tran-scription of genes whose protein products participate inantigen expression. HPV 16 E7 attenuated the ability of IFN-c to induce the transcription of several genes, and also of IRF-1, ... inhibits STAT1 expression and phosphorylationinduced by IFN-b and IFN-c in human KCs, and inhibits IRF-1, TAP1 and MHC class I expression inhost cells [29]. An impaired response of E7-KCs toIFN-c ... and irf-1) in KCs and E7-KCs both before and after exposure of the cells to IFN-c, using quantitative RT-PCR (Fig. 3).Basal levels of expression of pa-28 and irf-1 weresomewhat higher in E7-KCs...
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Báo cáo khoa học: A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea docx
... promotesassembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of ureaArnab Mukherjee, Manas K. Santra, Tushar K. Beuria and Dulal PandaSchool of Biosciences ... pHdependence of the urea and guanidine hydrochloridedenaturation of ribonuclease A and ribonuclease T1.Biochemistry 29, 2564–2572.62 Pace CN (1986) Determination and analysis of urea and guanidine ... fromDepartment of Science and Technology, Government of India (to DP). MKS and TKB were supported byfellowships from the Council of Scientific and Indus-trial Research, Government of India.References1...
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Báo cáo khoa học: WD-repeat-propeller-FYVE protein, ProF, binds VAMP2 and protein kinase Cf pptx
... mutants of ProF. Weanalyzed a Myc-tagged mutant of ProF, lacking theFYVE domain (ProFDFYVE) and two mutants lack-ing the FYVE domain and containing only blades 1–3(ProF 1–3) or blades 4–7 (ProF ... coexpressedthese proteins together with Flag-tagged VAMP2 inFig. 1. VAMP2, a new interaction partner of ProF. (A) Domain structure of ProF and VAMP2. ProF consists of seven WD repeats (WD1–7),binding to proteins, ... presence of increasingamounts of Myc-ProF in COS-7 cells (Fig. 3D). Ascan be seen, in the absence of Myc-ProF, only smallamounts of HA-PKCf were coprecipitated (Fig. 3D,lane 5). Coexpression of...
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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... 3 h of incubation at 37 °C and thenplated for selection of transformants. Protein techniques Protein was determined by protein- dye binding with BSA asa standard [47]. Whole cell protein of C. ... from the nrdF sequence of C. ammoniagenes ATCC 6872 [10] and subsequent purifica-tion of the IgG fraction. In-gel digestion of the R2F band and protein identification by Q-TOF MS-MS was performedas ... Brukersoftware, as described above.Analysis of metalsManganese and iron have been determined by GF-AAS and ICP-MS. [As a result of problems with the protein matrix inthe analysis of metalloproteins,...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
... times, aliquots of the culture filtrate of His+Mut+ cells were subjected toSDS ⁄ PAGE analysis and ACMSD activity determination.The time course of ACMSD expression in His+MutS cells was monitored ... verifyincorporation of the desired modification and to ensure theabsence of random mutations. The mutagenized plasmidswere transformed into P. pastoris GS115 competent cells and expression was performed ... software swiss-pdbviewer and visual molecular dynamics [49].AcknowledgementsWe thank G. Magni and S. Ruggieri for helpful com-ments and suggestions and T. Begley (Department of Chemistry and...
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Từ khóa: chuyên đề điện xoay chiều theo dạngNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Nghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roBT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ