Báo cáo khoa học: Recombinant expression of an insulin-like peptide 3 (INSL3) precursor and its enzymatic conversion to mature human INSL3 pot
Báo cáo khoa học: Recombinant expression of an insulin-like peptide 3 (INSL3) precursor and its enzymatic conversion to mature human INSL3 pot
... Recombinant expression of an insulin-like peptide 3 (INSL3) precursor and its enzymatic conversion to mature human INSL3 Xiao Luo 1 , Ross A. D. Bathgate 2 ,3 , Ya-Li Liu 4 , ... before and after in vitro refolding. (B) Far-UV spec- tra of the mature human INSL3 and porcine insulin. Fig. 7. cAMP activity of recombinant INSL3 and its pr...
Ngày tải lên: 30/03/2014, 01:20
Báo cáo khoa học: Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster pdf
... The use of mutant (e.g. deletion and extra copy numbers) and transgenic (RNAi and overexpres- sion) lines will help us to better understand the relationship between Hsps and cold tolerance. Experimental ... shock fac- tor mutant is accompanied by increased expression of Hsp 83 and DnaJ-1. Physiol Genomics 25, 4 93 501. 23 Bettencourt BR, Hogan CC, Nimali M & Drohan BW...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: Mechanistic investigation of a highly active phosphite dehydrogenase mutant and its application for NADPH regeneration pptx
... Interact 1 43 144, 533 –542. 41 Dunning Hotopp JC, Auchtung TA, Hogan DA & Hausinger RP (20 03) Intrinsic tryptophan fluorescence as a probe of metal and alpha-ketoglutarate binding to TfdA, a ... of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave, IL 61801, USA Fax: +1 217 33 35052 Tel: +1 217 33 32 631 E-mail: zhao5@ui...
Ngày tải lên: 23/03/2014, 15:20
Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
... tissues of rabbits at different time points (n =3) Tissues 2w 4w 6w Lungs 22.67±1. 53 18.67±1. 53 11.67±1. 53 Liver 26.67±1. 53 19.67±1. 53 13. 33 1. 53 Bonemarrow 40.00±4 .36 29.00±1.00 23. 33 1. 53 Normal ... Thera- peutic angiogenesis for patients with limb ischaemia by autol- ogous transplantation of bone-marrow cells: a pilot study and a randomised controlled trial. La...
Ngày tải lên: 25/10/2012, 11:18
Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt
... b-bar- rels of domains A and B (Fig. 2B), and had six hydro- gen bonds, three to the main chain carbonyls at Asn6, Leu168, and Val170, and three to the side chains of Arg11, Arg71, and Tyr216. The ... (Ala1 93, Leu297, Leu3 63, Phe371, Trp3 73, Ile427, Val428, Leu 430 , Trp508, and His509) and one hydrophilic resi- due, Asp 236 , form the binding pocket. The most evi- d...
Ngày tải lên: 14/02/2014, 18:20
Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... protein content, resulting in atomization temperatures of 230 0 °C and 2150 °C for manganese and iron, respectively. L-tryptophan-containing standards were used to simulate the protein background. In ... manganese analogue [4] of the iron containing class I RNR of Escherichia coli. This assignment was based on an analysis of its metal compo- sition and similarity of its...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... kinetics and mechanisms of the reactions of iron(III) with b-dik- etones. The formation of monoacetylacetoatoiron(III) and the effect of copper (II) on the formation of monothenoyltrifluoroacetonatoiron ... Germany) calibrated with Fe, Zn, Cu, Ni and Mn standards in the range £ 500 lgÆL )1 . The wavelengths used were 238 .204 nm (Fe), 2 13. 856 nm (Zn), 32 4.754 nm (Cu), 231...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: Complete reconstitution of an ATP-binding cassette transporter LolCDE complex from separately isolated subunits docx
... of E. coli is com- posed of one copy each of membrane subunits LolC and LolE, and two copies of ATPase subunit LolD [7]. Both LolC and LolE are assumed to span the mem- brane four times and to ... University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 1 13- 0 032 , Japan Fax: +81 3 5841 8464 Tel: +81 3 5841 7 830 E-mail: htokuda@iam.u-tokyo.ac.jp *Present address Depart...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt
... enzyme can be chan- ged by affecting rates of transcription and ⁄ or transla- tion and ⁄ or mRNA and protein degradation. This represents a quantitative strategy to offset the lack or excess of kinetic ... very often leads to chan- ges in two main traits of some metabolic enzymes: quantitative and qualitative. The quantitative properties (concentration and, as a consequence...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... like to thank L. Mayer and K. Uchida for pro- viding antibodies, A. do Vale, F. Pisarra and M.T. Sil- va for help and comments on TEM, and M. Santos and R. Hultcrantz for critical reading of the ... Sciot R, Moirand R, Jego P, Leroyer P & Brissot P (19 93) Regulation of ferritin and transferrin receptor expression by iron in human hepa- tocyte cultures. J Hepatol 18,...
Ngày tải lên: 19/02/2014, 16:20